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Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model

Reproductive and metabolic anomalies in polycystic ovary syndrome (PCOS) have been associated with the dysregulation of sex steroid receptors. Kelulut honey (KH) has been shown to be beneficial in PCOS-induced rats by regulating folliculogenesis and the oestrus cycle. However, no study has been cond...

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Autores principales: Kamal, Datu Agasi Mohd, Ibrahim, Siti Fatimah, Ugusman, Azizah, Mokhtar, Mohd Helmy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738483/
https://www.ncbi.nlm.nih.gov/pubmed/36499085
http://dx.doi.org/10.3390/ijms232314757
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author Kamal, Datu Agasi Mohd
Ibrahim, Siti Fatimah
Ugusman, Azizah
Mokhtar, Mohd Helmy
author_facet Kamal, Datu Agasi Mohd
Ibrahim, Siti Fatimah
Ugusman, Azizah
Mokhtar, Mohd Helmy
author_sort Kamal, Datu Agasi Mohd
collection PubMed
description Reproductive and metabolic anomalies in polycystic ovary syndrome (PCOS) have been associated with the dysregulation of sex steroid receptors. Kelulut honey (KH) has been shown to be beneficial in PCOS-induced rats by regulating folliculogenesis and the oestrus cycle. However, no study has been conducted to evaluate KH’s effect on sex steroid receptors in PCOS. Therefore, the current study examined the effects of KH, metformin, or clomiphene alone and in combination on the mRNA expression and protein distribution of androgen receptor (AR), oestrogen receptor α (ERα), oestrogen receptor β (ERβ), and progesterone receptor (PR) in PCOS-induced rats. The study used female Sprague-Dawley rats, which were treated orally with 1 mg/kg/day of letrozole for 21 days to develop PCOS. PCOS-induced rats were then divided and treated orally for 35 days with KH, metformin, clomiphene, KH + metformin, KH+ clomiphene and distilled water. In this study, we observed aberrant AR, ERα, ERβ and PR expression in PCOS-induced rats compared with the normal control rats. The effects of KH treatment were comparable with clomiphene and metformin in normalizing the expression of AR, ERα, and ERβ mRNA. However, KH, clomiphene and metformin did not affect PR mRNA expression and protein distribution. Hence, this study confirms the aberrant expression of sex steroid receptors in PCOS and demonstrates that KH treatment could normalise the sex steroid receptors profile. The findings provide a basis for future clinical trials to utilize KH as a regulator of sex steroid receptors in patients with PCOS.
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spelling pubmed-97384832022-12-11 Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model Kamal, Datu Agasi Mohd Ibrahim, Siti Fatimah Ugusman, Azizah Mokhtar, Mohd Helmy Int J Mol Sci Article Reproductive and metabolic anomalies in polycystic ovary syndrome (PCOS) have been associated with the dysregulation of sex steroid receptors. Kelulut honey (KH) has been shown to be beneficial in PCOS-induced rats by regulating folliculogenesis and the oestrus cycle. However, no study has been conducted to evaluate KH’s effect on sex steroid receptors in PCOS. Therefore, the current study examined the effects of KH, metformin, or clomiphene alone and in combination on the mRNA expression and protein distribution of androgen receptor (AR), oestrogen receptor α (ERα), oestrogen receptor β (ERβ), and progesterone receptor (PR) in PCOS-induced rats. The study used female Sprague-Dawley rats, which were treated orally with 1 mg/kg/day of letrozole for 21 days to develop PCOS. PCOS-induced rats were then divided and treated orally for 35 days with KH, metformin, clomiphene, KH + metformin, KH+ clomiphene and distilled water. In this study, we observed aberrant AR, ERα, ERβ and PR expression in PCOS-induced rats compared with the normal control rats. The effects of KH treatment were comparable with clomiphene and metformin in normalizing the expression of AR, ERα, and ERβ mRNA. However, KH, clomiphene and metformin did not affect PR mRNA expression and protein distribution. Hence, this study confirms the aberrant expression of sex steroid receptors in PCOS and demonstrates that KH treatment could normalise the sex steroid receptors profile. The findings provide a basis for future clinical trials to utilize KH as a regulator of sex steroid receptors in patients with PCOS. MDPI 2022-11-25 /pmc/articles/PMC9738483/ /pubmed/36499085 http://dx.doi.org/10.3390/ijms232314757 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kamal, Datu Agasi Mohd
Ibrahim, Siti Fatimah
Ugusman, Azizah
Mokhtar, Mohd Helmy
Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model
title Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model
title_full Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model
title_fullStr Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model
title_full_unstemmed Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model
title_short Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model
title_sort kelulut honey regulates sex steroid receptors in a polycystic ovary syndrome rat model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738483/
https://www.ncbi.nlm.nih.gov/pubmed/36499085
http://dx.doi.org/10.3390/ijms232314757
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