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Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response

Determination of metal ions such as zinc in solution remains an important task in analytical and biological chemistry. We describe a novel zinc ion biosensing approach using a carbonic anhydrase–Oplophorus luciferase fusion protein that employs bioluminescence resonance energy transfer (BRET) to tra...

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Autores principales: Matveeva, Evgenia G., Stoddard, Andrea K., Zeng, Hui-Hui, Franke, Graham, Bourne, Leslie, Fierke, Carol A., Thompson, Richard B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738544/
https://www.ncbi.nlm.nih.gov/pubmed/36499262
http://dx.doi.org/10.3390/ijms232314936
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author Matveeva, Evgenia G.
Stoddard, Andrea K.
Zeng, Hui-Hui
Franke, Graham
Bourne, Leslie
Fierke, Carol A.
Thompson, Richard B.
author_facet Matveeva, Evgenia G.
Stoddard, Andrea K.
Zeng, Hui-Hui
Franke, Graham
Bourne, Leslie
Fierke, Carol A.
Thompson, Richard B.
author_sort Matveeva, Evgenia G.
collection PubMed
description Determination of metal ions such as zinc in solution remains an important task in analytical and biological chemistry. We describe a novel zinc ion biosensing approach using a carbonic anhydrase–Oplophorus luciferase fusion protein that employs bioluminescence resonance energy transfer (BRET) to transduce the level of free zinc as a ratio of emission intensities in the blue and orange portions of the spectrum. In addition to high sensitivity (below nanomolar levels) and selectivity, this approach allows both quantitative determination of “free” zinc ion (also termed “mobile” or “labile”) using bioluminescence ratios and determination of the presence of the ion above a threshold simply by the change in color of bioluminescence, without an instrument. The carbonic anhydrase metal ion sensing platform offers well-established flexibility in sensitivity, selectivity, and response kinetics. Finally, bioluminescence labeling has proven an effective approach for molecular imaging in vivo since no exciting light is required; the expressible nature of this sensor offers the prospect of imaging zinc fluxes in vivo.
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spelling pubmed-97385442022-12-11 Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response Matveeva, Evgenia G. Stoddard, Andrea K. Zeng, Hui-Hui Franke, Graham Bourne, Leslie Fierke, Carol A. Thompson, Richard B. Int J Mol Sci Article Determination of metal ions such as zinc in solution remains an important task in analytical and biological chemistry. We describe a novel zinc ion biosensing approach using a carbonic anhydrase–Oplophorus luciferase fusion protein that employs bioluminescence resonance energy transfer (BRET) to transduce the level of free zinc as a ratio of emission intensities in the blue and orange portions of the spectrum. In addition to high sensitivity (below nanomolar levels) and selectivity, this approach allows both quantitative determination of “free” zinc ion (also termed “mobile” or “labile”) using bioluminescence ratios and determination of the presence of the ion above a threshold simply by the change in color of bioluminescence, without an instrument. The carbonic anhydrase metal ion sensing platform offers well-established flexibility in sensitivity, selectivity, and response kinetics. Finally, bioluminescence labeling has proven an effective approach for molecular imaging in vivo since no exciting light is required; the expressible nature of this sensor offers the prospect of imaging zinc fluxes in vivo. MDPI 2022-11-29 /pmc/articles/PMC9738544/ /pubmed/36499262 http://dx.doi.org/10.3390/ijms232314936 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matveeva, Evgenia G.
Stoddard, Andrea K.
Zeng, Hui-Hui
Franke, Graham
Bourne, Leslie
Fierke, Carol A.
Thompson, Richard B.
Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response
title Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response
title_full Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response
title_fullStr Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response
title_full_unstemmed Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response
title_short Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response
title_sort ratiometric zinc biosensor based on bioluminescence resonance energy transfer: trace metal ion determination with tunable response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738544/
https://www.ncbi.nlm.nih.gov/pubmed/36499262
http://dx.doi.org/10.3390/ijms232314936
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