Cargando…
Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response
Determination of metal ions such as zinc in solution remains an important task in analytical and biological chemistry. We describe a novel zinc ion biosensing approach using a carbonic anhydrase–Oplophorus luciferase fusion protein that employs bioluminescence resonance energy transfer (BRET) to tra...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738544/ https://www.ncbi.nlm.nih.gov/pubmed/36499262 http://dx.doi.org/10.3390/ijms232314936 |
_version_ | 1784847571356745728 |
---|---|
author | Matveeva, Evgenia G. Stoddard, Andrea K. Zeng, Hui-Hui Franke, Graham Bourne, Leslie Fierke, Carol A. Thompson, Richard B. |
author_facet | Matveeva, Evgenia G. Stoddard, Andrea K. Zeng, Hui-Hui Franke, Graham Bourne, Leslie Fierke, Carol A. Thompson, Richard B. |
author_sort | Matveeva, Evgenia G. |
collection | PubMed |
description | Determination of metal ions such as zinc in solution remains an important task in analytical and biological chemistry. We describe a novel zinc ion biosensing approach using a carbonic anhydrase–Oplophorus luciferase fusion protein that employs bioluminescence resonance energy transfer (BRET) to transduce the level of free zinc as a ratio of emission intensities in the blue and orange portions of the spectrum. In addition to high sensitivity (below nanomolar levels) and selectivity, this approach allows both quantitative determination of “free” zinc ion (also termed “mobile” or “labile”) using bioluminescence ratios and determination of the presence of the ion above a threshold simply by the change in color of bioluminescence, without an instrument. The carbonic anhydrase metal ion sensing platform offers well-established flexibility in sensitivity, selectivity, and response kinetics. Finally, bioluminescence labeling has proven an effective approach for molecular imaging in vivo since no exciting light is required; the expressible nature of this sensor offers the prospect of imaging zinc fluxes in vivo. |
format | Online Article Text |
id | pubmed-9738544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97385442022-12-11 Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response Matveeva, Evgenia G. Stoddard, Andrea K. Zeng, Hui-Hui Franke, Graham Bourne, Leslie Fierke, Carol A. Thompson, Richard B. Int J Mol Sci Article Determination of metal ions such as zinc in solution remains an important task in analytical and biological chemistry. We describe a novel zinc ion biosensing approach using a carbonic anhydrase–Oplophorus luciferase fusion protein that employs bioluminescence resonance energy transfer (BRET) to transduce the level of free zinc as a ratio of emission intensities in the blue and orange portions of the spectrum. In addition to high sensitivity (below nanomolar levels) and selectivity, this approach allows both quantitative determination of “free” zinc ion (also termed “mobile” or “labile”) using bioluminescence ratios and determination of the presence of the ion above a threshold simply by the change in color of bioluminescence, without an instrument. The carbonic anhydrase metal ion sensing platform offers well-established flexibility in sensitivity, selectivity, and response kinetics. Finally, bioluminescence labeling has proven an effective approach for molecular imaging in vivo since no exciting light is required; the expressible nature of this sensor offers the prospect of imaging zinc fluxes in vivo. MDPI 2022-11-29 /pmc/articles/PMC9738544/ /pubmed/36499262 http://dx.doi.org/10.3390/ijms232314936 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Matveeva, Evgenia G. Stoddard, Andrea K. Zeng, Hui-Hui Franke, Graham Bourne, Leslie Fierke, Carol A. Thompson, Richard B. Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response |
title | Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response |
title_full | Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response |
title_fullStr | Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response |
title_full_unstemmed | Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response |
title_short | Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response |
title_sort | ratiometric zinc biosensor based on bioluminescence resonance energy transfer: trace metal ion determination with tunable response |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738544/ https://www.ncbi.nlm.nih.gov/pubmed/36499262 http://dx.doi.org/10.3390/ijms232314936 |
work_keys_str_mv | AT matveevaevgeniag ratiometriczincbiosensorbasedonbioluminescenceresonanceenergytransfertracemetaliondeterminationwithtunableresponse AT stoddardandreak ratiometriczincbiosensorbasedonbioluminescenceresonanceenergytransfertracemetaliondeterminationwithtunableresponse AT zenghuihui ratiometriczincbiosensorbasedonbioluminescenceresonanceenergytransfertracemetaliondeterminationwithtunableresponse AT frankegraham ratiometriczincbiosensorbasedonbioluminescenceresonanceenergytransfertracemetaliondeterminationwithtunableresponse AT bourneleslie ratiometriczincbiosensorbasedonbioluminescenceresonanceenergytransfertracemetaliondeterminationwithtunableresponse AT fierkecarola ratiometriczincbiosensorbasedonbioluminescenceresonanceenergytransfertracemetaliondeterminationwithtunableresponse AT thompsonrichardb ratiometriczincbiosensorbasedonbioluminescenceresonanceenergytransfertracemetaliondeterminationwithtunableresponse |