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MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology

Nowadays, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is routinely implemented as the reference method for the swift and straightforward identification of microorganisms. However, this method is not flawless and there is a need to upgrade the current m...

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Detalles Bibliográficos
Autores principales: Feucherolles, Maureen, Frache, Gilles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738593/
https://www.ncbi.nlm.nih.gov/pubmed/36497158
http://dx.doi.org/10.3390/cells11233900
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author Feucherolles, Maureen
Frache, Gilles
author_facet Feucherolles, Maureen
Frache, Gilles
author_sort Feucherolles, Maureen
collection PubMed
description Nowadays, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is routinely implemented as the reference method for the swift and straightforward identification of microorganisms. However, this method is not flawless and there is a need to upgrade the current methodology in order to free the routine lab from incubation time and shift from a culture-dependent to an even faster independent culture system. Over the last two decades, mass spectrometry imaging (MSI) gained tremendous popularity in life sciences, including microbiology, due to its ability to simultaneously detect biomolecules, as well as their spatial distribution, in complex samples. Through this literature review, we summarize the latest applications of MALDI-MSI in microbiology. In addition, we discuss the challenges and avenues of exploration for applying MSI to solve current MALDI-TOF MS limits in routine and research laboratories.
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spelling pubmed-97385932022-12-11 MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology Feucherolles, Maureen Frache, Gilles Cells Review Nowadays, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is routinely implemented as the reference method for the swift and straightforward identification of microorganisms. However, this method is not flawless and there is a need to upgrade the current methodology in order to free the routine lab from incubation time and shift from a culture-dependent to an even faster independent culture system. Over the last two decades, mass spectrometry imaging (MSI) gained tremendous popularity in life sciences, including microbiology, due to its ability to simultaneously detect biomolecules, as well as their spatial distribution, in complex samples. Through this literature review, we summarize the latest applications of MALDI-MSI in microbiology. In addition, we discuss the challenges and avenues of exploration for applying MSI to solve current MALDI-TOF MS limits in routine and research laboratories. MDPI 2022-12-02 /pmc/articles/PMC9738593/ /pubmed/36497158 http://dx.doi.org/10.3390/cells11233900 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Feucherolles, Maureen
Frache, Gilles
MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology
title MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology
title_full MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology
title_fullStr MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology
title_full_unstemmed MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology
title_short MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology
title_sort maldi mass spectrometry imaging: a potential game-changer in a modern microbiology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738593/
https://www.ncbi.nlm.nih.gov/pubmed/36497158
http://dx.doi.org/10.3390/cells11233900
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