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MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology
Nowadays, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is routinely implemented as the reference method for the swift and straightforward identification of microorganisms. However, this method is not flawless and there is a need to upgrade the current m...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738593/ https://www.ncbi.nlm.nih.gov/pubmed/36497158 http://dx.doi.org/10.3390/cells11233900 |
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author | Feucherolles, Maureen Frache, Gilles |
author_facet | Feucherolles, Maureen Frache, Gilles |
author_sort | Feucherolles, Maureen |
collection | PubMed |
description | Nowadays, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is routinely implemented as the reference method for the swift and straightforward identification of microorganisms. However, this method is not flawless and there is a need to upgrade the current methodology in order to free the routine lab from incubation time and shift from a culture-dependent to an even faster independent culture system. Over the last two decades, mass spectrometry imaging (MSI) gained tremendous popularity in life sciences, including microbiology, due to its ability to simultaneously detect biomolecules, as well as their spatial distribution, in complex samples. Through this literature review, we summarize the latest applications of MALDI-MSI in microbiology. In addition, we discuss the challenges and avenues of exploration for applying MSI to solve current MALDI-TOF MS limits in routine and research laboratories. |
format | Online Article Text |
id | pubmed-9738593 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97385932022-12-11 MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology Feucherolles, Maureen Frache, Gilles Cells Review Nowadays, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is routinely implemented as the reference method for the swift and straightforward identification of microorganisms. However, this method is not flawless and there is a need to upgrade the current methodology in order to free the routine lab from incubation time and shift from a culture-dependent to an even faster independent culture system. Over the last two decades, mass spectrometry imaging (MSI) gained tremendous popularity in life sciences, including microbiology, due to its ability to simultaneously detect biomolecules, as well as their spatial distribution, in complex samples. Through this literature review, we summarize the latest applications of MALDI-MSI in microbiology. In addition, we discuss the challenges and avenues of exploration for applying MSI to solve current MALDI-TOF MS limits in routine and research laboratories. MDPI 2022-12-02 /pmc/articles/PMC9738593/ /pubmed/36497158 http://dx.doi.org/10.3390/cells11233900 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Feucherolles, Maureen Frache, Gilles MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology |
title | MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology |
title_full | MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology |
title_fullStr | MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology |
title_full_unstemmed | MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology |
title_short | MALDI Mass Spectrometry Imaging: A Potential Game-Changer in a Modern Microbiology |
title_sort | maldi mass spectrometry imaging: a potential game-changer in a modern microbiology |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738593/ https://www.ncbi.nlm.nih.gov/pubmed/36497158 http://dx.doi.org/10.3390/cells11233900 |
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