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Milk-Compositional Study of Metabolites and Pathogens in the Milk of Bovine Animals Affected with Subclinical Mastitis

Bovine milk is an important food component in the human diet due to its nutrient-rich metabolites. However, bovine subclinical mastitis alters the composition and quality of milk. In present study, California mastitis testing, somatic cell count, pH, and electrical conductivity were used as confirma...

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Autores principales: Ali, Aarif, Mir, Manzoor Ur Rahman, Ganie, Showkat Ahmad, Mushtaq, Saima, Bukhari, Sarah I., Alshehri, Sultan, Rashid, Shahzada Mudasir, Mir, Tahir Maqbool, Rehman, Muneeb U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738622/
https://www.ncbi.nlm.nih.gov/pubmed/36500724
http://dx.doi.org/10.3390/molecules27238631
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author Ali, Aarif
Mir, Manzoor Ur Rahman
Ganie, Showkat Ahmad
Mushtaq, Saima
Bukhari, Sarah I.
Alshehri, Sultan
Rashid, Shahzada Mudasir
Mir, Tahir Maqbool
Rehman, Muneeb U.
author_facet Ali, Aarif
Mir, Manzoor Ur Rahman
Ganie, Showkat Ahmad
Mushtaq, Saima
Bukhari, Sarah I.
Alshehri, Sultan
Rashid, Shahzada Mudasir
Mir, Tahir Maqbool
Rehman, Muneeb U.
author_sort Ali, Aarif
collection PubMed
description Bovine milk is an important food component in the human diet due to its nutrient-rich metabolites. However, bovine subclinical mastitis alters the composition and quality of milk. In present study, California mastitis testing, somatic cell count, pH, and electrical conductivity were used as confirmatory tests to detect subclinical mastitis. The primary goal was to study metabolome and identify major pathogens in cows with subclinical mastitis. In this study, 29 metabolites were detected in milk using gas chromatography–mass spectrometry. Volatile acidic compounds, such as hexanoic acid, hexadecanoic acid, lauric acid, octanoic acid, n-decanoic acid, tricosanoic acid, tetradecanoic acid, and hypogeic acid were found in milk samples, and these impart good flavor to the milk. Metaboanalyst tool was used for metabolic pathway analysis and principal component estimation. In this study, EC and pH values in milk were significantly increased (p < 0.0001), whereas fat (p < 0.04) and protein (p < 0.0002) significantly decreased in animals with subclinical mastitis in comparison to healthy animals. Staphylococcus aureus was the predominant pathogen found (n = 54), followed by Escherichia coli (n = 30). Furthermore, antibiotic sensitivity revealed that Staphylococcus aureus was more sensitive to gentamicin (79.6%), whereas Escherichia coli showed more sensitivity to doxycycline hydrochloride (80%).
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spelling pubmed-97386222022-12-11 Milk-Compositional Study of Metabolites and Pathogens in the Milk of Bovine Animals Affected with Subclinical Mastitis Ali, Aarif Mir, Manzoor Ur Rahman Ganie, Showkat Ahmad Mushtaq, Saima Bukhari, Sarah I. Alshehri, Sultan Rashid, Shahzada Mudasir Mir, Tahir Maqbool Rehman, Muneeb U. Molecules Article Bovine milk is an important food component in the human diet due to its nutrient-rich metabolites. However, bovine subclinical mastitis alters the composition and quality of milk. In present study, California mastitis testing, somatic cell count, pH, and electrical conductivity were used as confirmatory tests to detect subclinical mastitis. The primary goal was to study metabolome and identify major pathogens in cows with subclinical mastitis. In this study, 29 metabolites were detected in milk using gas chromatography–mass spectrometry. Volatile acidic compounds, such as hexanoic acid, hexadecanoic acid, lauric acid, octanoic acid, n-decanoic acid, tricosanoic acid, tetradecanoic acid, and hypogeic acid were found in milk samples, and these impart good flavor to the milk. Metaboanalyst tool was used for metabolic pathway analysis and principal component estimation. In this study, EC and pH values in milk were significantly increased (p < 0.0001), whereas fat (p < 0.04) and protein (p < 0.0002) significantly decreased in animals with subclinical mastitis in comparison to healthy animals. Staphylococcus aureus was the predominant pathogen found (n = 54), followed by Escherichia coli (n = 30). Furthermore, antibiotic sensitivity revealed that Staphylococcus aureus was more sensitive to gentamicin (79.6%), whereas Escherichia coli showed more sensitivity to doxycycline hydrochloride (80%). MDPI 2022-12-06 /pmc/articles/PMC9738622/ /pubmed/36500724 http://dx.doi.org/10.3390/molecules27238631 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ali, Aarif
Mir, Manzoor Ur Rahman
Ganie, Showkat Ahmad
Mushtaq, Saima
Bukhari, Sarah I.
Alshehri, Sultan
Rashid, Shahzada Mudasir
Mir, Tahir Maqbool
Rehman, Muneeb U.
Milk-Compositional Study of Metabolites and Pathogens in the Milk of Bovine Animals Affected with Subclinical Mastitis
title Milk-Compositional Study of Metabolites and Pathogens in the Milk of Bovine Animals Affected with Subclinical Mastitis
title_full Milk-Compositional Study of Metabolites and Pathogens in the Milk of Bovine Animals Affected with Subclinical Mastitis
title_fullStr Milk-Compositional Study of Metabolites and Pathogens in the Milk of Bovine Animals Affected with Subclinical Mastitis
title_full_unstemmed Milk-Compositional Study of Metabolites and Pathogens in the Milk of Bovine Animals Affected with Subclinical Mastitis
title_short Milk-Compositional Study of Metabolites and Pathogens in the Milk of Bovine Animals Affected with Subclinical Mastitis
title_sort milk-compositional study of metabolites and pathogens in the milk of bovine animals affected with subclinical mastitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738622/
https://www.ncbi.nlm.nih.gov/pubmed/36500724
http://dx.doi.org/10.3390/molecules27238631
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