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Endocannabinoid System Regulation in Female Rats with Recurrent Episodes of Binge Eating

Recurrent Binge Eating (BE) episodes characterize several eating disorders. Here, we attempted to reassemble a condition closer to BE disorder, and we analyzed whether recurrent episodes might evoke molecular alterations in the hypothalamus of rats. The hypothalamus is a brain region which is sensit...

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Autores principales: Pucci, Mariangela, D’Addario, Claudio, Micioni Di Bonaventura, Emanuela, Mercante, Francesca, Annunzi, Eugenia, Fanti, Federico, Sergi, Manuel, Botticelli, Luca, Einaudi, Giacomo, Cifani, Carlo, Micioni Di Bonaventura, Maria Vittoria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738776/
https://www.ncbi.nlm.nih.gov/pubmed/36499556
http://dx.doi.org/10.3390/ijms232315228
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author Pucci, Mariangela
D’Addario, Claudio
Micioni Di Bonaventura, Emanuela
Mercante, Francesca
Annunzi, Eugenia
Fanti, Federico
Sergi, Manuel
Botticelli, Luca
Einaudi, Giacomo
Cifani, Carlo
Micioni Di Bonaventura, Maria Vittoria
author_facet Pucci, Mariangela
D’Addario, Claudio
Micioni Di Bonaventura, Emanuela
Mercante, Francesca
Annunzi, Eugenia
Fanti, Federico
Sergi, Manuel
Botticelli, Luca
Einaudi, Giacomo
Cifani, Carlo
Micioni Di Bonaventura, Maria Vittoria
author_sort Pucci, Mariangela
collection PubMed
description Recurrent Binge Eating (BE) episodes characterize several eating disorders. Here, we attempted to reassemble a condition closer to BE disorder, and we analyzed whether recurrent episodes might evoke molecular alterations in the hypothalamus of rats. The hypothalamus is a brain region which is sensitive to stress and relevant in motivated behaviors, such as food intake. A well-characterized animal model of BE, in which a history of intermittent food restriction and stress induce binge-like palatable food consumption, was used to analyze the transcriptional regulation of the endocannabinoid system (ECS). We detected, in rats showing the BE behavior, an up-regulated gene expression of cannabinoid type-1 receptor (CB1), sn-1-specific diacylglycerol lipase, as well as fatty acid amide hydrolase (Faah) and monoacylglycerol lipase. A selective reduction in DNA methylation was also observed at the promoter of Faah, which is consistent with the changes in the gene expression. Moreover, BE behavior in rats was associated with an increase in anandamide (AEA) levels. Our findings support the relevant role of the ECS in the regulation of food intake in rats subjected to repeated BE episodes, and, in particular, on AEA signaling, acting via CB1 and FAAH modulation. Notably, the epigenetic regulation of the Faah gene might suggest this enzyme as a possible target for developing new therapeutical approaches.
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spelling pubmed-97387762022-12-11 Endocannabinoid System Regulation in Female Rats with Recurrent Episodes of Binge Eating Pucci, Mariangela D’Addario, Claudio Micioni Di Bonaventura, Emanuela Mercante, Francesca Annunzi, Eugenia Fanti, Federico Sergi, Manuel Botticelli, Luca Einaudi, Giacomo Cifani, Carlo Micioni Di Bonaventura, Maria Vittoria Int J Mol Sci Article Recurrent Binge Eating (BE) episodes characterize several eating disorders. Here, we attempted to reassemble a condition closer to BE disorder, and we analyzed whether recurrent episodes might evoke molecular alterations in the hypothalamus of rats. The hypothalamus is a brain region which is sensitive to stress and relevant in motivated behaviors, such as food intake. A well-characterized animal model of BE, in which a history of intermittent food restriction and stress induce binge-like palatable food consumption, was used to analyze the transcriptional regulation of the endocannabinoid system (ECS). We detected, in rats showing the BE behavior, an up-regulated gene expression of cannabinoid type-1 receptor (CB1), sn-1-specific diacylglycerol lipase, as well as fatty acid amide hydrolase (Faah) and monoacylglycerol lipase. A selective reduction in DNA methylation was also observed at the promoter of Faah, which is consistent with the changes in the gene expression. Moreover, BE behavior in rats was associated with an increase in anandamide (AEA) levels. Our findings support the relevant role of the ECS in the regulation of food intake in rats subjected to repeated BE episodes, and, in particular, on AEA signaling, acting via CB1 and FAAH modulation. Notably, the epigenetic regulation of the Faah gene might suggest this enzyme as a possible target for developing new therapeutical approaches. MDPI 2022-12-03 /pmc/articles/PMC9738776/ /pubmed/36499556 http://dx.doi.org/10.3390/ijms232315228 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pucci, Mariangela
D’Addario, Claudio
Micioni Di Bonaventura, Emanuela
Mercante, Francesca
Annunzi, Eugenia
Fanti, Federico
Sergi, Manuel
Botticelli, Luca
Einaudi, Giacomo
Cifani, Carlo
Micioni Di Bonaventura, Maria Vittoria
Endocannabinoid System Regulation in Female Rats with Recurrent Episodes of Binge Eating
title Endocannabinoid System Regulation in Female Rats with Recurrent Episodes of Binge Eating
title_full Endocannabinoid System Regulation in Female Rats with Recurrent Episodes of Binge Eating
title_fullStr Endocannabinoid System Regulation in Female Rats with Recurrent Episodes of Binge Eating
title_full_unstemmed Endocannabinoid System Regulation in Female Rats with Recurrent Episodes of Binge Eating
title_short Endocannabinoid System Regulation in Female Rats with Recurrent Episodes of Binge Eating
title_sort endocannabinoid system regulation in female rats with recurrent episodes of binge eating
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738776/
https://www.ncbi.nlm.nih.gov/pubmed/36499556
http://dx.doi.org/10.3390/ijms232315228
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