Relationship between Reticulorumen Parameters Measured in Real Time and Methane Emission and Heat Stress Risk in Dairy Cows

SIMPLE SUMMARY: We have found only a few publications in the literature on the link between rumen pH and CH(4). Based on this, we hypothesized that reticulorumen pH and temperature, where the latter is registered on an online system, affect greenhouse gas CH(4) emissions. As a result, to the best of our knowledge, this is the first study that has assessed the relationship of CH(4) emissions with reticulorumen pH and temperature. According to the aim of this study, we found that cows with a higher pH (6.22–6.42) produce 46.18% more methane emissions than cows with a lower pH. Moreover, cows with a higher risk of heat stress had a higher risk of subclinical acidosis. The novel aspect of the study is that, by using real-time reticulorumen pH, a temperature-registration system, and a laser methane detector, we could establish a relationship between reticulorumen parameters measured in real time and methane emissions and heat-stress risk in dairy cows. For this reason, more studies should be conducted to evaluate this process. ABSTRACT: The objective of this study was to investigate a connection between CH(4) emissions and reticulorumen pH and temperature. During the experiment, we registered the following parameters: reticulorumen pH (pH), reticulorumen temperature (RR temp.), reticulorumen temperature without drinking cycles, ambient temperature, ambient relative humidity, cow activity, heat index, temperature–humidity index (THI), and methane emissions (CH(4)). The experimental animals were divided into two groups based on the reticulorumen pH: 1. pH < 6.22 and 2. pH 6.22–6.42. We found that cows assigned to the second pH class had higher (46.18%) average values for methane emissions (p < 0.01). For the other indicators, higher average values were detected in cows of the first pH class, RR temperature (2.80%), relative humidity (20.96%), temperature–humidity index (2.47%) (p < 0.01), and temperature (3.93%) (p < 0.05), which were higher compared to cows of the second pH class. Reticulorumen pH was highly negatively correlated with THI and temperature (r = −0.667 to 0.717, p < 0.001) and somewhat negatively with heat index, relative humidity, and RR temperature (r = −0.536, p < 0.001; r = −0.471 to 0.456, p < 0.01). Cows with a higher risk of heat stress had a higher risk of lower reticulorumen pH.