Cargando…
Cathelicidin-Related Antimicrobial Peptide Negatively Regulates Bacterial Endotoxin-Induced Glial Activation
Recent studies have suggested that mouse cathelicidin-related antimicrobial peptide (CRAMP) and its human homologue leucine leucine-37 (LL-37) play critical roles in innate immune responses. Here, we studied the role of mouse CRAMP in bacterial endotoxin lipopolysaccharide (LPS)-induced neuroinflamm...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738883/ https://www.ncbi.nlm.nih.gov/pubmed/36497142 http://dx.doi.org/10.3390/cells11233886 |
_version_ | 1784847660669206528 |
---|---|
author | Bhusal, Anup Nam, Youngpyo Seo, Donggun Lee, Won-Ha Suk, Kyoungho |
author_facet | Bhusal, Anup Nam, Youngpyo Seo, Donggun Lee, Won-Ha Suk, Kyoungho |
author_sort | Bhusal, Anup |
collection | PubMed |
description | Recent studies have suggested that mouse cathelicidin-related antimicrobial peptide (CRAMP) and its human homologue leucine leucine-37 (LL-37) play critical roles in innate immune responses. Here, we studied the role of mouse CRAMP in bacterial endotoxin lipopolysaccharide (LPS)-induced neuroinflammation. CRAMP peptide treatment significantly inhibited LPS-mediated inflammatory activation of glial cells in culture. In the animal model of LPS-induced neuroinflammation, CRAMP expression was highly induced in multiple cell types, such as astrocytes, microglia, and neurons. Injection of exogenous CRAMP peptide significantly inhibited inflammatory cytokine expression and the reactivity of glial cells in the mouse brain following intraperitoneal or intracerebroventricular LPS administration. Altogether, results of the study suggest that CRAMP plays an important part in containment of LPS-induced neuroinflammatory responses, and that CRAMP can be exploited for the development of targeted therapies for neuroinflammatory conditions associated with bacterial infection. |
format | Online Article Text |
id | pubmed-9738883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97388832022-12-11 Cathelicidin-Related Antimicrobial Peptide Negatively Regulates Bacterial Endotoxin-Induced Glial Activation Bhusal, Anup Nam, Youngpyo Seo, Donggun Lee, Won-Ha Suk, Kyoungho Cells Article Recent studies have suggested that mouse cathelicidin-related antimicrobial peptide (CRAMP) and its human homologue leucine leucine-37 (LL-37) play critical roles in innate immune responses. Here, we studied the role of mouse CRAMP in bacterial endotoxin lipopolysaccharide (LPS)-induced neuroinflammation. CRAMP peptide treatment significantly inhibited LPS-mediated inflammatory activation of glial cells in culture. In the animal model of LPS-induced neuroinflammation, CRAMP expression was highly induced in multiple cell types, such as astrocytes, microglia, and neurons. Injection of exogenous CRAMP peptide significantly inhibited inflammatory cytokine expression and the reactivity of glial cells in the mouse brain following intraperitoneal or intracerebroventricular LPS administration. Altogether, results of the study suggest that CRAMP plays an important part in containment of LPS-induced neuroinflammatory responses, and that CRAMP can be exploited for the development of targeted therapies for neuroinflammatory conditions associated with bacterial infection. MDPI 2022-12-01 /pmc/articles/PMC9738883/ /pubmed/36497142 http://dx.doi.org/10.3390/cells11233886 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bhusal, Anup Nam, Youngpyo Seo, Donggun Lee, Won-Ha Suk, Kyoungho Cathelicidin-Related Antimicrobial Peptide Negatively Regulates Bacterial Endotoxin-Induced Glial Activation |
title | Cathelicidin-Related Antimicrobial Peptide Negatively Regulates Bacterial Endotoxin-Induced Glial Activation |
title_full | Cathelicidin-Related Antimicrobial Peptide Negatively Regulates Bacterial Endotoxin-Induced Glial Activation |
title_fullStr | Cathelicidin-Related Antimicrobial Peptide Negatively Regulates Bacterial Endotoxin-Induced Glial Activation |
title_full_unstemmed | Cathelicidin-Related Antimicrobial Peptide Negatively Regulates Bacterial Endotoxin-Induced Glial Activation |
title_short | Cathelicidin-Related Antimicrobial Peptide Negatively Regulates Bacterial Endotoxin-Induced Glial Activation |
title_sort | cathelicidin-related antimicrobial peptide negatively regulates bacterial endotoxin-induced glial activation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738883/ https://www.ncbi.nlm.nih.gov/pubmed/36497142 http://dx.doi.org/10.3390/cells11233886 |
work_keys_str_mv | AT bhusalanup cathelicidinrelatedantimicrobialpeptidenegativelyregulatesbacterialendotoxininducedglialactivation AT namyoungpyo cathelicidinrelatedantimicrobialpeptidenegativelyregulatesbacterialendotoxininducedglialactivation AT seodonggun cathelicidinrelatedantimicrobialpeptidenegativelyregulatesbacterialendotoxininducedglialactivation AT leewonha cathelicidinrelatedantimicrobialpeptidenegativelyregulatesbacterialendotoxininducedglialactivation AT sukkyoungho cathelicidinrelatedantimicrobialpeptidenegativelyregulatesbacterialendotoxininducedglialactivation |