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Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures
Advanced Therapy Medicinal Products are promising drugs for patients in therapeutic impasses. Their complex fabrication process implies regular quality controls to monitor cell concentration. Among the different methods available, optical techniques offer several advantages. Our study aims to measur...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738982/ https://www.ncbi.nlm.nih.gov/pubmed/36501924 http://dx.doi.org/10.3390/s22239223 |
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author | Wacogne, Bruno Vaccari, Naïs Koubevi, Claudia Belinger-Podevin, Marine Robert-Nicoud, Marjorie Rouleau, Alain Frelet-Barrand, Annie |
author_facet | Wacogne, Bruno Vaccari, Naïs Koubevi, Claudia Belinger-Podevin, Marine Robert-Nicoud, Marjorie Rouleau, Alain Frelet-Barrand, Annie |
author_sort | Wacogne, Bruno |
collection | PubMed |
description | Advanced Therapy Medicinal Products are promising drugs for patients in therapeutic impasses. Their complex fabrication process implies regular quality controls to monitor cell concentration. Among the different methods available, optical techniques offer several advantages. Our study aims to measure cell concentration in real time in a potential closed-loop environment using white light spectroscopy and to test the possibility of simultaneously measuring concentrations of several species. By analyzing the shapes of the absorption spectra, this system allowed the quantification of T-cells with an accuracy of about 3% during 30 h of cultivation monitoring and 26 h of doubling time, coherent with what is expected for normal cell culture. Moreover, our system permitted concentration measurements for two species in reconstructed co-cultures of T-cells and Candida albicans yeasts. This method can now be applied to any single or co-culture, it allows real-time monitoring, and can be easily integrated into a closed system. |
format | Online Article Text |
id | pubmed-9738982 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97389822022-12-11 Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures Wacogne, Bruno Vaccari, Naïs Koubevi, Claudia Belinger-Podevin, Marine Robert-Nicoud, Marjorie Rouleau, Alain Frelet-Barrand, Annie Sensors (Basel) Article Advanced Therapy Medicinal Products are promising drugs for patients in therapeutic impasses. Their complex fabrication process implies regular quality controls to monitor cell concentration. Among the different methods available, optical techniques offer several advantages. Our study aims to measure cell concentration in real time in a potential closed-loop environment using white light spectroscopy and to test the possibility of simultaneously measuring concentrations of several species. By analyzing the shapes of the absorption spectra, this system allowed the quantification of T-cells with an accuracy of about 3% during 30 h of cultivation monitoring and 26 h of doubling time, coherent with what is expected for normal cell culture. Moreover, our system permitted concentration measurements for two species in reconstructed co-cultures of T-cells and Candida albicans yeasts. This method can now be applied to any single or co-culture, it allows real-time monitoring, and can be easily integrated into a closed system. MDPI 2022-11-27 /pmc/articles/PMC9738982/ /pubmed/36501924 http://dx.doi.org/10.3390/s22239223 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wacogne, Bruno Vaccari, Naïs Koubevi, Claudia Belinger-Podevin, Marine Robert-Nicoud, Marjorie Rouleau, Alain Frelet-Barrand, Annie Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures |
title | Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures |
title_full | Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures |
title_fullStr | Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures |
title_full_unstemmed | Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures |
title_short | Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures |
title_sort | absorption spectra description for t-cell concentrations determination and simultaneous measurements of species during co-cultures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738982/ https://www.ncbi.nlm.nih.gov/pubmed/36501924 http://dx.doi.org/10.3390/s22239223 |
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