Cargando…

Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures

Advanced Therapy Medicinal Products are promising drugs for patients in therapeutic impasses. Their complex fabrication process implies regular quality controls to monitor cell concentration. Among the different methods available, optical techniques offer several advantages. Our study aims to measur...

Descripción completa

Detalles Bibliográficos
Autores principales: Wacogne, Bruno, Vaccari, Naïs, Koubevi, Claudia, Belinger-Podevin, Marine, Robert-Nicoud, Marjorie, Rouleau, Alain, Frelet-Barrand, Annie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738982/
https://www.ncbi.nlm.nih.gov/pubmed/36501924
http://dx.doi.org/10.3390/s22239223
_version_ 1784847686569033728
author Wacogne, Bruno
Vaccari, Naïs
Koubevi, Claudia
Belinger-Podevin, Marine
Robert-Nicoud, Marjorie
Rouleau, Alain
Frelet-Barrand, Annie
author_facet Wacogne, Bruno
Vaccari, Naïs
Koubevi, Claudia
Belinger-Podevin, Marine
Robert-Nicoud, Marjorie
Rouleau, Alain
Frelet-Barrand, Annie
author_sort Wacogne, Bruno
collection PubMed
description Advanced Therapy Medicinal Products are promising drugs for patients in therapeutic impasses. Their complex fabrication process implies regular quality controls to monitor cell concentration. Among the different methods available, optical techniques offer several advantages. Our study aims to measure cell concentration in real time in a potential closed-loop environment using white light spectroscopy and to test the possibility of simultaneously measuring concentrations of several species. By analyzing the shapes of the absorption spectra, this system allowed the quantification of T-cells with an accuracy of about 3% during 30 h of cultivation monitoring and 26 h of doubling time, coherent with what is expected for normal cell culture. Moreover, our system permitted concentration measurements for two species in reconstructed co-cultures of T-cells and Candida albicans yeasts. This method can now be applied to any single or co-culture, it allows real-time monitoring, and can be easily integrated into a closed system.
format Online
Article
Text
id pubmed-9738982
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97389822022-12-11 Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures Wacogne, Bruno Vaccari, Naïs Koubevi, Claudia Belinger-Podevin, Marine Robert-Nicoud, Marjorie Rouleau, Alain Frelet-Barrand, Annie Sensors (Basel) Article Advanced Therapy Medicinal Products are promising drugs for patients in therapeutic impasses. Their complex fabrication process implies regular quality controls to monitor cell concentration. Among the different methods available, optical techniques offer several advantages. Our study aims to measure cell concentration in real time in a potential closed-loop environment using white light spectroscopy and to test the possibility of simultaneously measuring concentrations of several species. By analyzing the shapes of the absorption spectra, this system allowed the quantification of T-cells with an accuracy of about 3% during 30 h of cultivation monitoring and 26 h of doubling time, coherent with what is expected for normal cell culture. Moreover, our system permitted concentration measurements for two species in reconstructed co-cultures of T-cells and Candida albicans yeasts. This method can now be applied to any single or co-culture, it allows real-time monitoring, and can be easily integrated into a closed system. MDPI 2022-11-27 /pmc/articles/PMC9738982/ /pubmed/36501924 http://dx.doi.org/10.3390/s22239223 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wacogne, Bruno
Vaccari, Naïs
Koubevi, Claudia
Belinger-Podevin, Marine
Robert-Nicoud, Marjorie
Rouleau, Alain
Frelet-Barrand, Annie
Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures
title Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures
title_full Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures
title_fullStr Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures
title_full_unstemmed Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures
title_short Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures
title_sort absorption spectra description for t-cell concentrations determination and simultaneous measurements of species during co-cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738982/
https://www.ncbi.nlm.nih.gov/pubmed/36501924
http://dx.doi.org/10.3390/s22239223
work_keys_str_mv AT wacognebruno absorptionspectradescriptionfortcellconcentrationsdeterminationandsimultaneousmeasurementsofspeciesduringcocultures
AT vaccarinais absorptionspectradescriptionfortcellconcentrationsdeterminationandsimultaneousmeasurementsofspeciesduringcocultures
AT koubeviclaudia absorptionspectradescriptionfortcellconcentrationsdeterminationandsimultaneousmeasurementsofspeciesduringcocultures
AT belingerpodevinmarine absorptionspectradescriptionfortcellconcentrationsdeterminationandsimultaneousmeasurementsofspeciesduringcocultures
AT robertnicoudmarjorie absorptionspectradescriptionfortcellconcentrationsdeterminationandsimultaneousmeasurementsofspeciesduringcocultures
AT rouleaualain absorptionspectradescriptionfortcellconcentrationsdeterminationandsimultaneousmeasurementsofspeciesduringcocultures
AT freletbarrandannie absorptionspectradescriptionfortcellconcentrationsdeterminationandsimultaneousmeasurementsofspeciesduringcocultures