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Transient Retention of Photoreceptor Outer Segments in Matrigel-Embedded Retinal Organoids
Retinal organoids (ROs) are three-dimensional retinal tissues, which are differentiated in vitro from induced pluripotent stem cells (iPSC), ultimately forming all main retinal cell types under defined culture conditions. ROs show several highly specialized retinal features, including the outgrowth...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739155/ https://www.ncbi.nlm.nih.gov/pubmed/36499228 http://dx.doi.org/10.3390/ijms232314893 |
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author | Berber, Patricia Bondarenko, Sofiia Michaelis, Lisa Weber, Bernhard Heinrich Friedrich |
author_facet | Berber, Patricia Bondarenko, Sofiia Michaelis, Lisa Weber, Bernhard Heinrich Friedrich |
author_sort | Berber, Patricia |
collection | PubMed |
description | Retinal organoids (ROs) are three-dimensional retinal tissues, which are differentiated in vitro from induced pluripotent stem cells (iPSC), ultimately forming all main retinal cell types under defined culture conditions. ROs show several highly specialized retinal features, including the outgrowth of photoreceptor outer segments (OSs). In vivo, the photoreceptor OSs are enveloped and maintained by protrusions of retinal pigment epithelium (RPE) cells, the so-called apical microvilli, while ROs fail to recapitulate this critical interaction in culture development. Here, we define specific co-culture conditions aiming to compensate for the missing physical proximity of RPE and OSs in RO development. Accordingly, functional RPE cells and ROs were differentiated simultaneously from the same iPSC clone, the former resulting in byproduct RPE or bRPE cells. While some co-culture approaches indicated a temporary functional interaction between bRPE and RO photoreceptors, they did not improve the photoreceptor histoarchitecture. In contrast, embedding ROs in a basement membrane extract without bRPE cells showed a robust improvement in the rate of photoreceptor OS retention. RO embedding is a quick and easy method that greatly enhances the preservation of photoreceptor OSs, an important structure for modelling retinal diseases with the involvement of photoreceptors. |
format | Online Article Text |
id | pubmed-9739155 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97391552022-12-11 Transient Retention of Photoreceptor Outer Segments in Matrigel-Embedded Retinal Organoids Berber, Patricia Bondarenko, Sofiia Michaelis, Lisa Weber, Bernhard Heinrich Friedrich Int J Mol Sci Article Retinal organoids (ROs) are three-dimensional retinal tissues, which are differentiated in vitro from induced pluripotent stem cells (iPSC), ultimately forming all main retinal cell types under defined culture conditions. ROs show several highly specialized retinal features, including the outgrowth of photoreceptor outer segments (OSs). In vivo, the photoreceptor OSs are enveloped and maintained by protrusions of retinal pigment epithelium (RPE) cells, the so-called apical microvilli, while ROs fail to recapitulate this critical interaction in culture development. Here, we define specific co-culture conditions aiming to compensate for the missing physical proximity of RPE and OSs in RO development. Accordingly, functional RPE cells and ROs were differentiated simultaneously from the same iPSC clone, the former resulting in byproduct RPE or bRPE cells. While some co-culture approaches indicated a temporary functional interaction between bRPE and RO photoreceptors, they did not improve the photoreceptor histoarchitecture. In contrast, embedding ROs in a basement membrane extract without bRPE cells showed a robust improvement in the rate of photoreceptor OS retention. RO embedding is a quick and easy method that greatly enhances the preservation of photoreceptor OSs, an important structure for modelling retinal diseases with the involvement of photoreceptors. MDPI 2022-11-28 /pmc/articles/PMC9739155/ /pubmed/36499228 http://dx.doi.org/10.3390/ijms232314893 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Berber, Patricia Bondarenko, Sofiia Michaelis, Lisa Weber, Bernhard Heinrich Friedrich Transient Retention of Photoreceptor Outer Segments in Matrigel-Embedded Retinal Organoids |
title | Transient Retention of Photoreceptor Outer Segments in Matrigel-Embedded Retinal Organoids |
title_full | Transient Retention of Photoreceptor Outer Segments in Matrigel-Embedded Retinal Organoids |
title_fullStr | Transient Retention of Photoreceptor Outer Segments in Matrigel-Embedded Retinal Organoids |
title_full_unstemmed | Transient Retention of Photoreceptor Outer Segments in Matrigel-Embedded Retinal Organoids |
title_short | Transient Retention of Photoreceptor Outer Segments in Matrigel-Embedded Retinal Organoids |
title_sort | transient retention of photoreceptor outer segments in matrigel-embedded retinal organoids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739155/ https://www.ncbi.nlm.nih.gov/pubmed/36499228 http://dx.doi.org/10.3390/ijms232314893 |
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