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Dynamic Light Scattering Plus Scanning Electron Microscopy: Usefulness and Limitations of a Simplified Estimation of Nanocellulose Dimensions

Measurements of nanocellulose size usually demand very high-resolution techniques and tedious image processing, mainly in what pertains to the length of nanofibers. Aiming to ease the process, this work assesses a relatively simple method to estimate the dimensions of nanocellulose particles with an...

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Autores principales: Tarrés, Quim, Aguado, Roberto, Zoppe, Justin O., Mutjé, Pere, Fiol, Núria, Delgado-Aguilar, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739265/
https://www.ncbi.nlm.nih.gov/pubmed/36500912
http://dx.doi.org/10.3390/nano12234288
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author Tarrés, Quim
Aguado, Roberto
Zoppe, Justin O.
Mutjé, Pere
Fiol, Núria
Delgado-Aguilar, Marc
author_facet Tarrés, Quim
Aguado, Roberto
Zoppe, Justin O.
Mutjé, Pere
Fiol, Núria
Delgado-Aguilar, Marc
author_sort Tarrés, Quim
collection PubMed
description Measurements of nanocellulose size usually demand very high-resolution techniques and tedious image processing, mainly in what pertains to the length of nanofibers. Aiming to ease the process, this work assesses a relatively simple method to estimate the dimensions of nanocellulose particles with an aspect ratio greater than 1. Nanocellulose suspensions, both as nanofibers and as nanocrystals, are subjected to dynamic light scattering (DLS) and to field-emission scanning electron microscopy (FE-SEM). The former provides the hydrodynamic diameter, as long as the scatter angle and the consistency are adequate. Assays with different angles and concentrations compel us to recommend forward scattering (12.8°) and concentrations around 0.05–0.10 wt %. Then, FE-SEM with magnifications of ×5000–×20,000 generally suffices to obtain an acceptable approximation for the actual diameter, at least for bundles. Finally, length can be estimated by a simple geometric relationship. Regardless of whether they are collected from FE-SEM or DLS, size distributions are generally skewed to lower diameters. Width distributions from FE-SEM, in particular, are well fitted to log-normal functions. Overall, while this method is not valid for the thinnest fibrils or for single, small nanocrystals, it can be useful in lieu of very high-resolution techniques.
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spelling pubmed-97392652022-12-11 Dynamic Light Scattering Plus Scanning Electron Microscopy: Usefulness and Limitations of a Simplified Estimation of Nanocellulose Dimensions Tarrés, Quim Aguado, Roberto Zoppe, Justin O. Mutjé, Pere Fiol, Núria Delgado-Aguilar, Marc Nanomaterials (Basel) Article Measurements of nanocellulose size usually demand very high-resolution techniques and tedious image processing, mainly in what pertains to the length of nanofibers. Aiming to ease the process, this work assesses a relatively simple method to estimate the dimensions of nanocellulose particles with an aspect ratio greater than 1. Nanocellulose suspensions, both as nanofibers and as nanocrystals, are subjected to dynamic light scattering (DLS) and to field-emission scanning electron microscopy (FE-SEM). The former provides the hydrodynamic diameter, as long as the scatter angle and the consistency are adequate. Assays with different angles and concentrations compel us to recommend forward scattering (12.8°) and concentrations around 0.05–0.10 wt %. Then, FE-SEM with magnifications of ×5000–×20,000 generally suffices to obtain an acceptable approximation for the actual diameter, at least for bundles. Finally, length can be estimated by a simple geometric relationship. Regardless of whether they are collected from FE-SEM or DLS, size distributions are generally skewed to lower diameters. Width distributions from FE-SEM, in particular, are well fitted to log-normal functions. Overall, while this method is not valid for the thinnest fibrils or for single, small nanocrystals, it can be useful in lieu of very high-resolution techniques. MDPI 2022-12-02 /pmc/articles/PMC9739265/ /pubmed/36500912 http://dx.doi.org/10.3390/nano12234288 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tarrés, Quim
Aguado, Roberto
Zoppe, Justin O.
Mutjé, Pere
Fiol, Núria
Delgado-Aguilar, Marc
Dynamic Light Scattering Plus Scanning Electron Microscopy: Usefulness and Limitations of a Simplified Estimation of Nanocellulose Dimensions
title Dynamic Light Scattering Plus Scanning Electron Microscopy: Usefulness and Limitations of a Simplified Estimation of Nanocellulose Dimensions
title_full Dynamic Light Scattering Plus Scanning Electron Microscopy: Usefulness and Limitations of a Simplified Estimation of Nanocellulose Dimensions
title_fullStr Dynamic Light Scattering Plus Scanning Electron Microscopy: Usefulness and Limitations of a Simplified Estimation of Nanocellulose Dimensions
title_full_unstemmed Dynamic Light Scattering Plus Scanning Electron Microscopy: Usefulness and Limitations of a Simplified Estimation of Nanocellulose Dimensions
title_short Dynamic Light Scattering Plus Scanning Electron Microscopy: Usefulness and Limitations of a Simplified Estimation of Nanocellulose Dimensions
title_sort dynamic light scattering plus scanning electron microscopy: usefulness and limitations of a simplified estimation of nanocellulose dimensions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739265/
https://www.ncbi.nlm.nih.gov/pubmed/36500912
http://dx.doi.org/10.3390/nano12234288
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