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Tear and Plasma Levels of Cytokines in Patients with Uveitis: Search for Active Disease Biomarkers

Uveitis accounts for up to 20% of blindness in Europe, making the development of new non-invasive biomarkers which could help in its management a field of interest. It has been hypothesised that tear levels of cytokines and chemokines could be used as a potential biomarker in patients with anterior...

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Autores principales: García-Onrubia, Luis, Mateos Olivares, Milagros, García-Vázquez, Carmen, Enríquez-de-Salamanca, Amalia, Cocho, Lidia, Herreras Cantalapiedra, José María
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739309/
https://www.ncbi.nlm.nih.gov/pubmed/36498608
http://dx.doi.org/10.3390/jcm11237034
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author García-Onrubia, Luis
Mateos Olivares, Milagros
García-Vázquez, Carmen
Enríquez-de-Salamanca, Amalia
Cocho, Lidia
Herreras Cantalapiedra, José María
author_facet García-Onrubia, Luis
Mateos Olivares, Milagros
García-Vázquez, Carmen
Enríquez-de-Salamanca, Amalia
Cocho, Lidia
Herreras Cantalapiedra, José María
author_sort García-Onrubia, Luis
collection PubMed
description Uveitis accounts for up to 20% of blindness in Europe, making the development of new non-invasive biomarkers which could help in its management a field of interest. It has been hypothesised that tear levels of cytokines and chemokines could be used as a potential biomarker in patients with anterior uveitis, and this could be correlated with their concentration in plasma. Therefore, we measured twelve cytokines/chemokines in tear and plasma samples of 22 patients diagnosed with active anterior uveitis. Levels of these molecules in tears and plasma were compared and associated with the degree of activity of the uveitis. It is notable that the percentage of tear interleukin (IL)-6 detection was significantly reduced in the inactive phase (p < 0.05). However, the tear concentration in epidermal growth factor (EGF), fractalkine, IL-8, IL-1RA, interferon-inducible protein (IP)-10/CXCL10, vascular endothelial growth factor (VEGF) and IL-6, comparing the active and inactive period, was not statistically different. Apart from the tear VEGF levels, the cytokine/chemokine concentration in tears in the active/inactive phase was statistically different (p < 0.05) from the counterpart levels in plasma. In conclusion, no isolated cytokine/chemokine in the tears has been found in a concentration which could be used as a potential biomarker of disease activity and treatment response.
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spelling pubmed-97393092022-12-11 Tear and Plasma Levels of Cytokines in Patients with Uveitis: Search for Active Disease Biomarkers García-Onrubia, Luis Mateos Olivares, Milagros García-Vázquez, Carmen Enríquez-de-Salamanca, Amalia Cocho, Lidia Herreras Cantalapiedra, José María J Clin Med Article Uveitis accounts for up to 20% of blindness in Europe, making the development of new non-invasive biomarkers which could help in its management a field of interest. It has been hypothesised that tear levels of cytokines and chemokines could be used as a potential biomarker in patients with anterior uveitis, and this could be correlated with their concentration in plasma. Therefore, we measured twelve cytokines/chemokines in tear and plasma samples of 22 patients diagnosed with active anterior uveitis. Levels of these molecules in tears and plasma were compared and associated with the degree of activity of the uveitis. It is notable that the percentage of tear interleukin (IL)-6 detection was significantly reduced in the inactive phase (p < 0.05). However, the tear concentration in epidermal growth factor (EGF), fractalkine, IL-8, IL-1RA, interferon-inducible protein (IP)-10/CXCL10, vascular endothelial growth factor (VEGF) and IL-6, comparing the active and inactive period, was not statistically different. Apart from the tear VEGF levels, the cytokine/chemokine concentration in tears in the active/inactive phase was statistically different (p < 0.05) from the counterpart levels in plasma. In conclusion, no isolated cytokine/chemokine in the tears has been found in a concentration which could be used as a potential biomarker of disease activity and treatment response. MDPI 2022-11-28 /pmc/articles/PMC9739309/ /pubmed/36498608 http://dx.doi.org/10.3390/jcm11237034 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
García-Onrubia, Luis
Mateos Olivares, Milagros
García-Vázquez, Carmen
Enríquez-de-Salamanca, Amalia
Cocho, Lidia
Herreras Cantalapiedra, José María
Tear and Plasma Levels of Cytokines in Patients with Uveitis: Search for Active Disease Biomarkers
title Tear and Plasma Levels of Cytokines in Patients with Uveitis: Search for Active Disease Biomarkers
title_full Tear and Plasma Levels of Cytokines in Patients with Uveitis: Search for Active Disease Biomarkers
title_fullStr Tear and Plasma Levels of Cytokines in Patients with Uveitis: Search for Active Disease Biomarkers
title_full_unstemmed Tear and Plasma Levels of Cytokines in Patients with Uveitis: Search for Active Disease Biomarkers
title_short Tear and Plasma Levels of Cytokines in Patients with Uveitis: Search for Active Disease Biomarkers
title_sort tear and plasma levels of cytokines in patients with uveitis: search for active disease biomarkers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739309/
https://www.ncbi.nlm.nih.gov/pubmed/36498608
http://dx.doi.org/10.3390/jcm11237034
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