Heterologous Expression of Extracellular Proteinase pAsPs of Aspergillus pseudotamarii in Komagataella phaffii

Neutral protease pAsPs gene was obtained by sequence optimization of NpI protease from Aspergillus pseudotamarii. pAsPs was for the first time integrated in the genome of yeast strain Komagataella phaffii T07, and then produced in a 5 L bioreactor with an enzyme yield of 150,800 U/mL of culture liqu...

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Detalles Bibliográficos
Autores principales: Zadorozhny, Andrey Valentinovich, Voskoboev, Mikhail Evgenyevich, Bochkov, Denis Vladimirovich, Rozanov, Alexei Sergeyevich, Shedko, Elizaveta Dmitrievna, Mescheryakova, Irina Anatolyevna, Blinov, Alexander Gennadyevich, Korzhuk, Anton Vladimirovich, Shlyakhtun, Valeria Nikolayevna, Bogacheva, Natalia Vladimirovna, Antonov, Egor Vladimirovich, Bannikova, Svetlana Valerevna, Goryachkovskaya, Tatiana Nikolayevna, Peltek, Sergey Evgenyevich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739362/
https://www.ncbi.nlm.nih.gov/pubmed/36499360
http://dx.doi.org/10.3390/ijms232315035

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