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pH-Dependent Release of Vancomycin from Modularly Assembled Collagen Laminates

To prevent surgical site infections, antibiotics can be released from carriers made of biomaterials, such as collagen, that support the healing process and are slowly degraded in the body. In our labs we have developed collagen laminates that can be easily assembled and bonded on-site, according to...

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Autores principales: Kilb, Michelle Fiona, Ritz, Ulrike, Nickel, Daniela, Schmitz, Katja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740012/
https://www.ncbi.nlm.nih.gov/pubmed/36501621
http://dx.doi.org/10.3390/polym14235227
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author Kilb, Michelle Fiona
Ritz, Ulrike
Nickel, Daniela
Schmitz, Katja
author_facet Kilb, Michelle Fiona
Ritz, Ulrike
Nickel, Daniela
Schmitz, Katja
author_sort Kilb, Michelle Fiona
collection PubMed
description To prevent surgical site infections, antibiotics can be released from carriers made of biomaterials, such as collagen, that support the healing process and are slowly degraded in the body. In our labs we have developed collagen laminates that can be easily assembled and bonded on-site, according to medical needs. As shown previously, the asymmetric assembly leads to different release rates at the major faces of the laminate. Since the pH changes during the wound healing and infection, we further examined the effect of an acidic and alkaline pH, in comparison to pH 7.4 on the release of vancomycin from different collagen samples. For this purpose, we used an additively manufactured sample holder and quantified the release by HPLC. Our results show that the pH value does not have any influence on the total amount of released vancomycin (atelocollagen sponge pH 5.5: 71 ± 2%, pH 7.4: 68 ± 8%, pH 8.5: 74 ± 3%, bilayer laminate pH 5.5: 61 ± 6%, pH 7.4: 69 ± 4% and pH 8.5: 67 ± 3%) but on the time for half-maximal release. At an acidic pH of 5.5, the swelling of the atelocollagen sponge is largely increased, leading to a 2–3 h retarded release, compared to the physiological pH. No changes in swelling were observed at the basic pH and the compound release was 1–2 h delayed. These effects need to be considered when choosing the materials for the laminate assembly.
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spelling pubmed-97400122022-12-11 pH-Dependent Release of Vancomycin from Modularly Assembled Collagen Laminates Kilb, Michelle Fiona Ritz, Ulrike Nickel, Daniela Schmitz, Katja Polymers (Basel) Communication To prevent surgical site infections, antibiotics can be released from carriers made of biomaterials, such as collagen, that support the healing process and are slowly degraded in the body. In our labs we have developed collagen laminates that can be easily assembled and bonded on-site, according to medical needs. As shown previously, the asymmetric assembly leads to different release rates at the major faces of the laminate. Since the pH changes during the wound healing and infection, we further examined the effect of an acidic and alkaline pH, in comparison to pH 7.4 on the release of vancomycin from different collagen samples. For this purpose, we used an additively manufactured sample holder and quantified the release by HPLC. Our results show that the pH value does not have any influence on the total amount of released vancomycin (atelocollagen sponge pH 5.5: 71 ± 2%, pH 7.4: 68 ± 8%, pH 8.5: 74 ± 3%, bilayer laminate pH 5.5: 61 ± 6%, pH 7.4: 69 ± 4% and pH 8.5: 67 ± 3%) but on the time for half-maximal release. At an acidic pH of 5.5, the swelling of the atelocollagen sponge is largely increased, leading to a 2–3 h retarded release, compared to the physiological pH. No changes in swelling were observed at the basic pH and the compound release was 1–2 h delayed. These effects need to be considered when choosing the materials for the laminate assembly. MDPI 2022-12-01 /pmc/articles/PMC9740012/ /pubmed/36501621 http://dx.doi.org/10.3390/polym14235227 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kilb, Michelle Fiona
Ritz, Ulrike
Nickel, Daniela
Schmitz, Katja
pH-Dependent Release of Vancomycin from Modularly Assembled Collagen Laminates
title pH-Dependent Release of Vancomycin from Modularly Assembled Collagen Laminates
title_full pH-Dependent Release of Vancomycin from Modularly Assembled Collagen Laminates
title_fullStr pH-Dependent Release of Vancomycin from Modularly Assembled Collagen Laminates
title_full_unstemmed pH-Dependent Release of Vancomycin from Modularly Assembled Collagen Laminates
title_short pH-Dependent Release of Vancomycin from Modularly Assembled Collagen Laminates
title_sort ph-dependent release of vancomycin from modularly assembled collagen laminates
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740012/
https://www.ncbi.nlm.nih.gov/pubmed/36501621
http://dx.doi.org/10.3390/polym14235227
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