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Molecular Level Sucrose Quantification: A Critical Review

Sucrose is a primary metabolite in plants, a source of energy, a source of carbon atoms for growth and development, and a regulator of biochemical processes. Most of the traditional analytical chemistry methods for sucrose quantification in plants require sample treatment (with consequent tissue des...

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Autores principales: Lara-Cruz, Gustavo A., Jaramillo-Botero, Andres
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740140/
https://www.ncbi.nlm.nih.gov/pubmed/36502213
http://dx.doi.org/10.3390/s22239511
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author Lara-Cruz, Gustavo A.
Jaramillo-Botero, Andres
author_facet Lara-Cruz, Gustavo A.
Jaramillo-Botero, Andres
author_sort Lara-Cruz, Gustavo A.
collection PubMed
description Sucrose is a primary metabolite in plants, a source of energy, a source of carbon atoms for growth and development, and a regulator of biochemical processes. Most of the traditional analytical chemistry methods for sucrose quantification in plants require sample treatment (with consequent tissue destruction) and complex facilities, that do not allow real-time sucrose quantification at ultra-low concentrations (nM to pM range) under in vivo conditions, limiting our understanding of sucrose roles in plant physiology across different plant tissues and cellular compartments. Some of the above-mentioned problems may be circumvented with the use of bio-compatible ligands for molecular recognition of sucrose. Nevertheless, problems such as the signal-noise ratio, stability, and selectivity are some of the main challenges limiting the use of molecular recognition methods for the in vivo quantification of sucrose. In this review, we provide a critical analysis of the existing analytical chemistry tools, biosensors, and synthetic ligands, for sucrose quantification and discuss the most promising paths to improve upon its limits of detection. Our goal is to highlight the criteria design need for real-time, in vivo, highly sensitive and selective sucrose sensing capabilities to enable further our understanding of living organisms, the development of new plant breeding strategies for increased crop productivity and sustainability, and ultimately to contribute to the overarching need for food security.
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spelling pubmed-97401402022-12-11 Molecular Level Sucrose Quantification: A Critical Review Lara-Cruz, Gustavo A. Jaramillo-Botero, Andres Sensors (Basel) Review Sucrose is a primary metabolite in plants, a source of energy, a source of carbon atoms for growth and development, and a regulator of biochemical processes. Most of the traditional analytical chemistry methods for sucrose quantification in plants require sample treatment (with consequent tissue destruction) and complex facilities, that do not allow real-time sucrose quantification at ultra-low concentrations (nM to pM range) under in vivo conditions, limiting our understanding of sucrose roles in plant physiology across different plant tissues and cellular compartments. Some of the above-mentioned problems may be circumvented with the use of bio-compatible ligands for molecular recognition of sucrose. Nevertheless, problems such as the signal-noise ratio, stability, and selectivity are some of the main challenges limiting the use of molecular recognition methods for the in vivo quantification of sucrose. In this review, we provide a critical analysis of the existing analytical chemistry tools, biosensors, and synthetic ligands, for sucrose quantification and discuss the most promising paths to improve upon its limits of detection. Our goal is to highlight the criteria design need for real-time, in vivo, highly sensitive and selective sucrose sensing capabilities to enable further our understanding of living organisms, the development of new plant breeding strategies for increased crop productivity and sustainability, and ultimately to contribute to the overarching need for food security. MDPI 2022-12-05 /pmc/articles/PMC9740140/ /pubmed/36502213 http://dx.doi.org/10.3390/s22239511 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Lara-Cruz, Gustavo A.
Jaramillo-Botero, Andres
Molecular Level Sucrose Quantification: A Critical Review
title Molecular Level Sucrose Quantification: A Critical Review
title_full Molecular Level Sucrose Quantification: A Critical Review
title_fullStr Molecular Level Sucrose Quantification: A Critical Review
title_full_unstemmed Molecular Level Sucrose Quantification: A Critical Review
title_short Molecular Level Sucrose Quantification: A Critical Review
title_sort molecular level sucrose quantification: a critical review
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740140/
https://www.ncbi.nlm.nih.gov/pubmed/36502213
http://dx.doi.org/10.3390/s22239511
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