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Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of Myrtus communis L.
Natural products have offered a number of exciting approaches in cancer treatment over the years. In this study, we investigated the prophylactic and therapeutic effects of the polyphenol-enriched fraction extracted from Myrtus communis (PEMC) on acute and chronic leukemia. According to the UHPLC-MS...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740279/ https://www.ncbi.nlm.nih.gov/pubmed/36501085 http://dx.doi.org/10.3390/nu14235055 |
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author | Mechchate, Hamza de Castro Alves, Carlos Eduardo Es-safi, Imane Amaghnouje, Amal Jawhari, Fatima Zahra Costa de Oliveira, Regiane de Freitas Gomes, Alice Conte, Raffaele Soares Pontes, Gemilson Bousta, Dalila Grafov, Andriy |
author_facet | Mechchate, Hamza de Castro Alves, Carlos Eduardo Es-safi, Imane Amaghnouje, Amal Jawhari, Fatima Zahra Costa de Oliveira, Regiane de Freitas Gomes, Alice Conte, Raffaele Soares Pontes, Gemilson Bousta, Dalila Grafov, Andriy |
author_sort | Mechchate, Hamza |
collection | PubMed |
description | Natural products have offered a number of exciting approaches in cancer treatment over the years. In this study, we investigated the prophylactic and therapeutic effects of the polyphenol-enriched fraction extracted from Myrtus communis (PEMC) on acute and chronic leukemia. According to the UHPLC-MS(n), the fraction is rich in flavonoids. Protective activity of the PEMC was assessed by evaluating the antioxidant, anti-inflammatory, wound healing, and hemolysis potential in a series of in vivo and in vitro assays, while the therapeutic approach consisted of the evaluation of cytotoxic activity of the PEMC against HL60 and K562 leukemia cell lines. Safety of the fraction was also evaluated on a non-cancerous Vero cell line and by an acute toxicity test performed in mice. The PEMC demonstrated a significant anti-inflammatory and healing potential. The activities found at the dose of 100 mg/kg were better than those observed using a reference drug. The PEMC demonstrated a significant antioxidant effect and a specific cytotoxicity towards HL60 (IC(50) = 19.87 µM) and K562 (IC(50) = 29.64 µM) cell lines being non-toxic to the Vero cell line. No hemolytic activity was observed in vitro and no toxicity effect was found in mice. Thus, the PEMC has a pharmacological potential as both preventive and therapeutic agent. However, further research is necessary to propose its mechanism of action. |
format | Online Article Text |
id | pubmed-9740279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97402792022-12-11 Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of Myrtus communis L. Mechchate, Hamza de Castro Alves, Carlos Eduardo Es-safi, Imane Amaghnouje, Amal Jawhari, Fatima Zahra Costa de Oliveira, Regiane de Freitas Gomes, Alice Conte, Raffaele Soares Pontes, Gemilson Bousta, Dalila Grafov, Andriy Nutrients Article Natural products have offered a number of exciting approaches in cancer treatment over the years. In this study, we investigated the prophylactic and therapeutic effects of the polyphenol-enriched fraction extracted from Myrtus communis (PEMC) on acute and chronic leukemia. According to the UHPLC-MS(n), the fraction is rich in flavonoids. Protective activity of the PEMC was assessed by evaluating the antioxidant, anti-inflammatory, wound healing, and hemolysis potential in a series of in vivo and in vitro assays, while the therapeutic approach consisted of the evaluation of cytotoxic activity of the PEMC against HL60 and K562 leukemia cell lines. Safety of the fraction was also evaluated on a non-cancerous Vero cell line and by an acute toxicity test performed in mice. The PEMC demonstrated a significant anti-inflammatory and healing potential. The activities found at the dose of 100 mg/kg were better than those observed using a reference drug. The PEMC demonstrated a significant antioxidant effect and a specific cytotoxicity towards HL60 (IC(50) = 19.87 µM) and K562 (IC(50) = 29.64 µM) cell lines being non-toxic to the Vero cell line. No hemolytic activity was observed in vitro and no toxicity effect was found in mice. Thus, the PEMC has a pharmacological potential as both preventive and therapeutic agent. However, further research is necessary to propose its mechanism of action. MDPI 2022-11-27 /pmc/articles/PMC9740279/ /pubmed/36501085 http://dx.doi.org/10.3390/nu14235055 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mechchate, Hamza de Castro Alves, Carlos Eduardo Es-safi, Imane Amaghnouje, Amal Jawhari, Fatima Zahra Costa de Oliveira, Regiane de Freitas Gomes, Alice Conte, Raffaele Soares Pontes, Gemilson Bousta, Dalila Grafov, Andriy Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of Myrtus communis L. |
title | Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of Myrtus communis L. |
title_full | Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of Myrtus communis L. |
title_fullStr | Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of Myrtus communis L. |
title_full_unstemmed | Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of Myrtus communis L. |
title_short | Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of Myrtus communis L. |
title_sort | antileukemic, antioxidant, anti-inflammatory and healing activities induced by a polyphenol-enriched fraction extracted from leaves of myrtus communis l. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740279/ https://www.ncbi.nlm.nih.gov/pubmed/36501085 http://dx.doi.org/10.3390/nu14235055 |
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