Cytoophidia Maintain the Integrity of Drosophila Follicle Epithelium

CTP synthase (CTPS) forms a filamentous structure termed the cytoophidium in all three domains of life. The female reproductive system of Drosophila is an excellent model for studying the physiological function of cytoophidia. Here, we use CTPS(H355A), a point mutation that destroys the cytoophidium-forming ability of CTPS, to explore the in vivo function of cytoophidia. In CTPS(H355A) egg chambers, we observe the ingression and increased heterogeneity of follicle cells. In addition, we find that the cytoophidium-forming ability of CTPS, rather than the protein level, is the cause of the defects observed in CTPS(H355A) mutants. To sum up, our data indicate that cytoophidia play an important role in maintaining the integrity of follicle epithelium.