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Cytoophidia Maintain the Integrity of Drosophila Follicle Epithelium

CTP synthase (CTPS) forms a filamentous structure termed the cytoophidium in all three domains of life. The female reproductive system of Drosophila is an excellent model for studying the physiological function of cytoophidia. Here, we use CTPS(H355A), a point mutation that destroys the cytoophidium...

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Detalles Bibliográficos
Autores principales: Wang, Qiao-Qi, You, Dong-Dong, Liu, Ji-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740582/
https://www.ncbi.nlm.nih.gov/pubmed/36499609
http://dx.doi.org/10.3390/ijms232315282
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author Wang, Qiao-Qi
You, Dong-Dong
Liu, Ji-Long
author_facet Wang, Qiao-Qi
You, Dong-Dong
Liu, Ji-Long
author_sort Wang, Qiao-Qi
collection PubMed
description CTP synthase (CTPS) forms a filamentous structure termed the cytoophidium in all three domains of life. The female reproductive system of Drosophila is an excellent model for studying the physiological function of cytoophidia. Here, we use CTPS(H355A), a point mutation that destroys the cytoophidium-forming ability of CTPS, to explore the in vivo function of cytoophidia. In CTPS(H355A) egg chambers, we observe the ingression and increased heterogeneity of follicle cells. In addition, we find that the cytoophidium-forming ability of CTPS, rather than the protein level, is the cause of the defects observed in CTPS(H355A) mutants. To sum up, our data indicate that cytoophidia play an important role in maintaining the integrity of follicle epithelium.
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spelling pubmed-97405822022-12-11 Cytoophidia Maintain the Integrity of Drosophila Follicle Epithelium Wang, Qiao-Qi You, Dong-Dong Liu, Ji-Long Int J Mol Sci Article CTP synthase (CTPS) forms a filamentous structure termed the cytoophidium in all three domains of life. The female reproductive system of Drosophila is an excellent model for studying the physiological function of cytoophidia. Here, we use CTPS(H355A), a point mutation that destroys the cytoophidium-forming ability of CTPS, to explore the in vivo function of cytoophidia. In CTPS(H355A) egg chambers, we observe the ingression and increased heterogeneity of follicle cells. In addition, we find that the cytoophidium-forming ability of CTPS, rather than the protein level, is the cause of the defects observed in CTPS(H355A) mutants. To sum up, our data indicate that cytoophidia play an important role in maintaining the integrity of follicle epithelium. MDPI 2022-12-04 /pmc/articles/PMC9740582/ /pubmed/36499609 http://dx.doi.org/10.3390/ijms232315282 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Qiao-Qi
You, Dong-Dong
Liu, Ji-Long
Cytoophidia Maintain the Integrity of Drosophila Follicle Epithelium
title Cytoophidia Maintain the Integrity of Drosophila Follicle Epithelium
title_full Cytoophidia Maintain the Integrity of Drosophila Follicle Epithelium
title_fullStr Cytoophidia Maintain the Integrity of Drosophila Follicle Epithelium
title_full_unstemmed Cytoophidia Maintain the Integrity of Drosophila Follicle Epithelium
title_short Cytoophidia Maintain the Integrity of Drosophila Follicle Epithelium
title_sort cytoophidia maintain the integrity of drosophila follicle epithelium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740582/
https://www.ncbi.nlm.nih.gov/pubmed/36499609
http://dx.doi.org/10.3390/ijms232315282
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