Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner....

Descripción completa

Detalles Bibliográficos
Autores principales: Siddika, Mst. Ayesha, Yamada, Takahiro, Aoyama, Risako, Hidaka, Kumi, Sugiyama, Hiroshi, Endo, Masayuki, Matsumura, Shigeyoshi, Ikawa, Yoshiya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740620/
https://www.ncbi.nlm.nih.gov/pubmed/36500390
http://dx.doi.org/10.3390/molecules27238298
Descripción
Sumario:Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).

Ejemplares similares