Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner....

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Autores principales: Siddika, Mst. Ayesha, Yamada, Takahiro, Aoyama, Risako, Hidaka, Kumi, Sugiyama, Hiroshi, Endo, Masayuki, Matsumura, Shigeyoshi, Ikawa, Yoshiya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740620/
https://www.ncbi.nlm.nih.gov/pubmed/36500390
http://dx.doi.org/10.3390/molecules27238298
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author Siddika, Mst. Ayesha
Yamada, Takahiro
Aoyama, Risako
Hidaka, Kumi
Sugiyama, Hiroshi
Endo, Masayuki
Matsumura, Shigeyoshi
Ikawa, Yoshiya
author_facet Siddika, Mst. Ayesha
Yamada, Takahiro
Aoyama, Risako
Hidaka, Kumi
Sugiyama, Hiroshi
Endo, Masayuki
Matsumura, Shigeyoshi
Ikawa, Yoshiya
author_sort Siddika, Mst. Ayesha
collection PubMed
description Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).
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spelling pubmed-97406202022-12-11 Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes Siddika, Mst. Ayesha Yamada, Takahiro Aoyama, Risako Hidaka, Kumi Sugiyama, Hiroshi Endo, Masayuki Matsumura, Shigeyoshi Ikawa, Yoshiya Molecules Article Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM). MDPI 2022-11-28 /pmc/articles/PMC9740620/ /pubmed/36500390 http://dx.doi.org/10.3390/molecules27238298 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Siddika, Mst. Ayesha
Yamada, Takahiro
Aoyama, Risako
Hidaka, Kumi
Sugiyama, Hiroshi
Endo, Masayuki
Matsumura, Shigeyoshi
Ikawa, Yoshiya
Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes
title Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes
title_full Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes
title_fullStr Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes
title_full_unstemmed Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes
title_short Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes
title_sort catalytic rna oligomers formed by co-oligomerization of a pair of bimolecular rnase p ribozymes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740620/
https://www.ncbi.nlm.nih.gov/pubmed/36500390
http://dx.doi.org/10.3390/molecules27238298
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