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Fabrication of Chitosan-Based Network Polysaccharide Nanogels

In this study, we developed a method to fabricate chitosan-based network polysaccharides via the condensation between amino groups in water-soluble chitosan (WSCS) and a carboxylate-terminated maltooligosaccharide crosslinker. We previously reported on the fabrication of network-polysaccharide-based...

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Autores principales: Nakamichi, Aina, Kadokawa, Jun-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740819/
https://www.ncbi.nlm.nih.gov/pubmed/36500476
http://dx.doi.org/10.3390/molecules27238384
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author Nakamichi, Aina
Kadokawa, Jun-ichi
author_facet Nakamichi, Aina
Kadokawa, Jun-ichi
author_sort Nakamichi, Aina
collection PubMed
description In this study, we developed a method to fabricate chitosan-based network polysaccharides via the condensation between amino groups in water-soluble chitosan (WSCS) and a carboxylate-terminated maltooligosaccharide crosslinker. We previously reported on the fabrication of network-polysaccharide-based macroscopic hydrogels via the chemical crosslinking of water-soluble chitin (WSCh) with the crosslinker. Because the molecular weight of the WSCS was much smaller than that of the WSCh, in the present investigation, the chemical crosslinking of the WSCS with the crosslinker was observed at the nanoscale upon the condensation between amino and carboxylate groups in the presence of a condensing agent, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N-hydroxysuccinimide, affording nano-sized chitosan-based network polysaccharides. The occurrence of the crosslinking via the formation of amido linkages was supported by the IR analysis and (1)H NMR measurements after the dissolution via acid hydrolysis in DCl/D(2)O. The products formed nanogels, whose sizes depended on the amino/carboxylate feed ratio. The nanoscale morphology and size of the products were evaluated via scanning electron microscopy, dynamic light scattering analyses, and transition electron microscopy. In the present study, we successfully developed the method to fabricate nanogel materials based on network polysaccharide structures, which can practically be applied as new polysaccharide-based 3D bionanomaterials.
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spelling pubmed-97408192022-12-11 Fabrication of Chitosan-Based Network Polysaccharide Nanogels Nakamichi, Aina Kadokawa, Jun-ichi Molecules Communication In this study, we developed a method to fabricate chitosan-based network polysaccharides via the condensation between amino groups in water-soluble chitosan (WSCS) and a carboxylate-terminated maltooligosaccharide crosslinker. We previously reported on the fabrication of network-polysaccharide-based macroscopic hydrogels via the chemical crosslinking of water-soluble chitin (WSCh) with the crosslinker. Because the molecular weight of the WSCS was much smaller than that of the WSCh, in the present investigation, the chemical crosslinking of the WSCS with the crosslinker was observed at the nanoscale upon the condensation between amino and carboxylate groups in the presence of a condensing agent, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N-hydroxysuccinimide, affording nano-sized chitosan-based network polysaccharides. The occurrence of the crosslinking via the formation of amido linkages was supported by the IR analysis and (1)H NMR measurements after the dissolution via acid hydrolysis in DCl/D(2)O. The products formed nanogels, whose sizes depended on the amino/carboxylate feed ratio. The nanoscale morphology and size of the products were evaluated via scanning electron microscopy, dynamic light scattering analyses, and transition electron microscopy. In the present study, we successfully developed the method to fabricate nanogel materials based on network polysaccharide structures, which can practically be applied as new polysaccharide-based 3D bionanomaterials. MDPI 2022-12-01 /pmc/articles/PMC9740819/ /pubmed/36500476 http://dx.doi.org/10.3390/molecules27238384 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Nakamichi, Aina
Kadokawa, Jun-ichi
Fabrication of Chitosan-Based Network Polysaccharide Nanogels
title Fabrication of Chitosan-Based Network Polysaccharide Nanogels
title_full Fabrication of Chitosan-Based Network Polysaccharide Nanogels
title_fullStr Fabrication of Chitosan-Based Network Polysaccharide Nanogels
title_full_unstemmed Fabrication of Chitosan-Based Network Polysaccharide Nanogels
title_short Fabrication of Chitosan-Based Network Polysaccharide Nanogels
title_sort fabrication of chitosan-based network polysaccharide nanogels
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740819/
https://www.ncbi.nlm.nih.gov/pubmed/36500476
http://dx.doi.org/10.3390/molecules27238384
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