Cargando…

Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues

Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of cold-active enzymes that enable them to perform...

Descripción completa

Detalles Bibliográficos
Autores principales: Matinja, Adamu Idris, Kamarudin, Nor Hafizah Ahmad, Leow, Adam Thean Chor, Oslan, Siti Nurbaya, Ali, Mohd Shukuri Mohamad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740821/
https://www.ncbi.nlm.nih.gov/pubmed/36499718
http://dx.doi.org/10.3390/ijms232315394
_version_ 1784848161454424064
author Matinja, Adamu Idris
Kamarudin, Nor Hafizah Ahmad
Leow, Adam Thean Chor
Oslan, Siti Nurbaya
Ali, Mohd Shukuri Mohamad
author_facet Matinja, Adamu Idris
Kamarudin, Nor Hafizah Ahmad
Leow, Adam Thean Chor
Oslan, Siti Nurbaya
Ali, Mohd Shukuri Mohamad
author_sort Matinja, Adamu Idris
collection PubMed
description Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of cold-active enzymes that enable them to perform an efficient metabolic flux at low temperatures necessary to thrive and reproduce under those constraints. Cold-active enzymes are ideal biocatalysts that can reduce the need for heating procedures and improve industrial processes’ quality, sustainability, and cost-effectiveness. Despite their wide applications, their industrial usage is still limited, and the major contributing factor is the lack of complete understanding of their structure and cold adaptation mechanisms. The current review looked at the recombinant overexpression, purification, and recent mechanism of cold adaptation, various approaches for purification, and three-dimensional (3D) crystal structure elucidation of cold-active lipases and esterase.
format Online
Article
Text
id pubmed-9740821
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97408212022-12-11 Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues Matinja, Adamu Idris Kamarudin, Nor Hafizah Ahmad Leow, Adam Thean Chor Oslan, Siti Nurbaya Ali, Mohd Shukuri Mohamad Int J Mol Sci Review Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of cold-active enzymes that enable them to perform an efficient metabolic flux at low temperatures necessary to thrive and reproduce under those constraints. Cold-active enzymes are ideal biocatalysts that can reduce the need for heating procedures and improve industrial processes’ quality, sustainability, and cost-effectiveness. Despite their wide applications, their industrial usage is still limited, and the major contributing factor is the lack of complete understanding of their structure and cold adaptation mechanisms. The current review looked at the recombinant overexpression, purification, and recent mechanism of cold adaptation, various approaches for purification, and three-dimensional (3D) crystal structure elucidation of cold-active lipases and esterase. MDPI 2022-12-06 /pmc/articles/PMC9740821/ /pubmed/36499718 http://dx.doi.org/10.3390/ijms232315394 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Matinja, Adamu Idris
Kamarudin, Nor Hafizah Ahmad
Leow, Adam Thean Chor
Oslan, Siti Nurbaya
Ali, Mohd Shukuri Mohamad
Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues
title Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues
title_full Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues
title_fullStr Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues
title_full_unstemmed Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues
title_short Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues
title_sort cold-active lipases and esterases: a review on recombinant overexpression and other essential issues
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740821/
https://www.ncbi.nlm.nih.gov/pubmed/36499718
http://dx.doi.org/10.3390/ijms232315394
work_keys_str_mv AT matinjaadamuidris coldactivelipasesandesterasesareviewonrecombinantoverexpressionandotheressentialissues
AT kamarudinnorhafizahahmad coldactivelipasesandesterasesareviewonrecombinantoverexpressionandotheressentialissues
AT leowadamtheanchor coldactivelipasesandesterasesareviewonrecombinantoverexpressionandotheressentialissues
AT oslansitinurbaya coldactivelipasesandesterasesareviewonrecombinantoverexpressionandotheressentialissues
AT alimohdshukurimohamad coldactivelipasesandesterasesareviewonrecombinantoverexpressionandotheressentialissues