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Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues
Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of cold-active enzymes that enable them to perform...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740821/ https://www.ncbi.nlm.nih.gov/pubmed/36499718 http://dx.doi.org/10.3390/ijms232315394 |
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author | Matinja, Adamu Idris Kamarudin, Nor Hafizah Ahmad Leow, Adam Thean Chor Oslan, Siti Nurbaya Ali, Mohd Shukuri Mohamad |
author_facet | Matinja, Adamu Idris Kamarudin, Nor Hafizah Ahmad Leow, Adam Thean Chor Oslan, Siti Nurbaya Ali, Mohd Shukuri Mohamad |
author_sort | Matinja, Adamu Idris |
collection | PubMed |
description | Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of cold-active enzymes that enable them to perform an efficient metabolic flux at low temperatures necessary to thrive and reproduce under those constraints. Cold-active enzymes are ideal biocatalysts that can reduce the need for heating procedures and improve industrial processes’ quality, sustainability, and cost-effectiveness. Despite their wide applications, their industrial usage is still limited, and the major contributing factor is the lack of complete understanding of their structure and cold adaptation mechanisms. The current review looked at the recombinant overexpression, purification, and recent mechanism of cold adaptation, various approaches for purification, and three-dimensional (3D) crystal structure elucidation of cold-active lipases and esterase. |
format | Online Article Text |
id | pubmed-9740821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97408212022-12-11 Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues Matinja, Adamu Idris Kamarudin, Nor Hafizah Ahmad Leow, Adam Thean Chor Oslan, Siti Nurbaya Ali, Mohd Shukuri Mohamad Int J Mol Sci Review Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of cold-active enzymes that enable them to perform an efficient metabolic flux at low temperatures necessary to thrive and reproduce under those constraints. Cold-active enzymes are ideal biocatalysts that can reduce the need for heating procedures and improve industrial processes’ quality, sustainability, and cost-effectiveness. Despite their wide applications, their industrial usage is still limited, and the major contributing factor is the lack of complete understanding of their structure and cold adaptation mechanisms. The current review looked at the recombinant overexpression, purification, and recent mechanism of cold adaptation, various approaches for purification, and three-dimensional (3D) crystal structure elucidation of cold-active lipases and esterase. MDPI 2022-12-06 /pmc/articles/PMC9740821/ /pubmed/36499718 http://dx.doi.org/10.3390/ijms232315394 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Matinja, Adamu Idris Kamarudin, Nor Hafizah Ahmad Leow, Adam Thean Chor Oslan, Siti Nurbaya Ali, Mohd Shukuri Mohamad Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues |
title | Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues |
title_full | Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues |
title_fullStr | Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues |
title_full_unstemmed | Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues |
title_short | Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues |
title_sort | cold-active lipases and esterases: a review on recombinant overexpression and other essential issues |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9740821/ https://www.ncbi.nlm.nih.gov/pubmed/36499718 http://dx.doi.org/10.3390/ijms232315394 |
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