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Spectrum and Density of Gamma and X-ray Induced Mutations in a Non-Model Rice Cultivar

Physical mutagens are a powerful tool used for genetic research and breeding for over eight decades. Yet, when compared to chemical mutagens, data sets on the effect of different mutagens and dosages on the spectrum and density of induced mutations remain lacking. To address this, we investigated th...

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Autores principales: Jankowicz-Cieslak, Joanna, Hofinger, Bernhard J., Jarc, Luka, Junttila, Sini, Galik, Bence, Gyenesei, Attila, Ingelbrecht, Ivan L., Till, Bradley J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9741009/
https://www.ncbi.nlm.nih.gov/pubmed/36501272
http://dx.doi.org/10.3390/plants11233232
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author Jankowicz-Cieslak, Joanna
Hofinger, Bernhard J.
Jarc, Luka
Junttila, Sini
Galik, Bence
Gyenesei, Attila
Ingelbrecht, Ivan L.
Till, Bradley J.
author_facet Jankowicz-Cieslak, Joanna
Hofinger, Bernhard J.
Jarc, Luka
Junttila, Sini
Galik, Bence
Gyenesei, Attila
Ingelbrecht, Ivan L.
Till, Bradley J.
author_sort Jankowicz-Cieslak, Joanna
collection PubMed
description Physical mutagens are a powerful tool used for genetic research and breeding for over eight decades. Yet, when compared to chemical mutagens, data sets on the effect of different mutagens and dosages on the spectrum and density of induced mutations remain lacking. To address this, we investigated the landscape of mutations induced by gamma and X-ray radiation in the most widely cultivated crop species: rice. A mutant population of a tropical upland rice, Oryza sativa L., was generated and propagated via self-fertilization for seven generations. Five dosages ranging from 75 Gy to 600 Gy in both X-ray and gamma-irradiated material were applied. In the process of a forward genetic screens, 11 unique rice mutant lines showing phenotypic variation were selected for mutation analysis via whole-genome sequencing. Thousands of candidate mutations were recovered in each mutant with single base substitutions being the most common, followed by small indels and structural variants. Higher dosages resulted in a higher accumulation of mutations in gamma-irradiated material, but not in X-ray-treated plants. The in vivo role of all annotated rice genes is yet to be directly investigated. The ability to induce a high density of single nucleotide and structural variants through mutagenesis will likely remain an important approach for functional genomics and breeding.
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spelling pubmed-97410092022-12-11 Spectrum and Density of Gamma and X-ray Induced Mutations in a Non-Model Rice Cultivar Jankowicz-Cieslak, Joanna Hofinger, Bernhard J. Jarc, Luka Junttila, Sini Galik, Bence Gyenesei, Attila Ingelbrecht, Ivan L. Till, Bradley J. Plants (Basel) Article Physical mutagens are a powerful tool used for genetic research and breeding for over eight decades. Yet, when compared to chemical mutagens, data sets on the effect of different mutagens and dosages on the spectrum and density of induced mutations remain lacking. To address this, we investigated the landscape of mutations induced by gamma and X-ray radiation in the most widely cultivated crop species: rice. A mutant population of a tropical upland rice, Oryza sativa L., was generated and propagated via self-fertilization for seven generations. Five dosages ranging from 75 Gy to 600 Gy in both X-ray and gamma-irradiated material were applied. In the process of a forward genetic screens, 11 unique rice mutant lines showing phenotypic variation were selected for mutation analysis via whole-genome sequencing. Thousands of candidate mutations were recovered in each mutant with single base substitutions being the most common, followed by small indels and structural variants. Higher dosages resulted in a higher accumulation of mutations in gamma-irradiated material, but not in X-ray-treated plants. The in vivo role of all annotated rice genes is yet to be directly investigated. The ability to induce a high density of single nucleotide and structural variants through mutagenesis will likely remain an important approach for functional genomics and breeding. MDPI 2022-11-25 /pmc/articles/PMC9741009/ /pubmed/36501272 http://dx.doi.org/10.3390/plants11233232 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jankowicz-Cieslak, Joanna
Hofinger, Bernhard J.
Jarc, Luka
Junttila, Sini
Galik, Bence
Gyenesei, Attila
Ingelbrecht, Ivan L.
Till, Bradley J.
Spectrum and Density of Gamma and X-ray Induced Mutations in a Non-Model Rice Cultivar
title Spectrum and Density of Gamma and X-ray Induced Mutations in a Non-Model Rice Cultivar
title_full Spectrum and Density of Gamma and X-ray Induced Mutations in a Non-Model Rice Cultivar
title_fullStr Spectrum and Density of Gamma and X-ray Induced Mutations in a Non-Model Rice Cultivar
title_full_unstemmed Spectrum and Density of Gamma and X-ray Induced Mutations in a Non-Model Rice Cultivar
title_short Spectrum and Density of Gamma and X-ray Induced Mutations in a Non-Model Rice Cultivar
title_sort spectrum and density of gamma and x-ray induced mutations in a non-model rice cultivar
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9741009/
https://www.ncbi.nlm.nih.gov/pubmed/36501272
http://dx.doi.org/10.3390/plants11233232
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