Hemiurid Trematodes (Digenea: Hemiuridae) from Marine Fishes off the Coast of Rio de Janeiro, Brazil, with Novel Molecular Data

SIMPLE SUMMARY: Brazil, with its remarkably diverse marine habitats, harbour one of the world’s richest fish diversities. Consequently, the diversity of their trematode parasites is also expected to be extremely rich. However, our current knowledge on this group of animals is incomplete and there are many unknown trematode species that await discovery and genetic characterisation. The Hemiuridae (Digenea) is the second most speciose trematode family in marine fishes from Brazil; however, to date, it remains understudied. We examined forty-three specimens of nine fish species belonging to eight families (Carangidae, Clupeidae, Haemulidae, Muraenidae, Percophidae, Pinguipedidae, Trichiuridae, and Triglidae) collected from the coastal zone off Rio de Janeiro, Brazil and found hemiurid trematodes in the stomach of 14 fishes. Using morphological and molecular analyses, we identified eight species from four genera of the family Hemiuridae. One of these species is reported in Brazil for the first time, four are reported from new fish hosts, and four were genetically characterised for the first time. Our novel data contributes to the knowledge on marine biodiversity in Brazil and will further contribute to the classification of the family Hemiuridae. ABSTRACT: Brazil is a tropical country with remarkably diverse marine habitats that harbour a rich diversity of fish. Only a small portion of this fish diversity has been investigated for parasites, and thus the diversity of their trematode parasites remains unexplored. Moreover, only 5 out of 184 known digenean trematode species of marine fish in Brazil have been genetically characterised. The Hemiuridae Looss, 1899 is the second most speciose trematode family in marine fishes from Brazil but, in many ways, it remains a neglected group. Forty-three trematode specimens from nine fish species were collected from the coastal zone off Rio de Janeiro, Brazil. Trematodes were found in the stomach of 14 specimens of 9 fish species belonging to 8 families (Carangidae, Clupeidae, Haemulidae, Muraenidae, Percophidae, Pinguipedidae, Trichiuridae, and Triglidae). Trematode specimens were studied using morphological and molecular genetic analyses. A total of eight hemiurid species from four genera, Ectenurus, Lecithochirium, Myosaccium, and Parahemiurus were identified. This paper reports on new host records for four species of hemiurids, adds a new record on the geographical distribution for one species, and provides the first DNA sequence data supplemented with the detailed description of morphology for five species. Phylogenetic analyses supported that the subfamily classifications of the Hemiuridae—based entirely on morphological characters—needs to be reconsidered, taking into account a wider range of information sources.