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Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae in a Tertiary Hospital in Northern China

BACKGROUND: In recent years, carbapenem-resistant Klebsiella pneumoniae (CRKP) has emerged rapidly in China with the abuse and overuse of antibiotics, and infections caused by CRKP pose a serious threat to global public health safety. The present study aimed to explore the epidemiological characteri...

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Autores principales: Wang, Shuqing, Dong, Huanhuan, Wang, Meiqi, Ma, Wenbo, Cheng, Yue, Zhou, Junliang, Cheng, Yongming, Xu, Hui, Yu, Xiaochen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9741528/
https://www.ncbi.nlm.nih.gov/pubmed/36510603
http://dx.doi.org/10.1155/2022/2615753
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author Wang, Shuqing
Dong, Huanhuan
Wang, Meiqi
Ma, Wenbo
Cheng, Yue
Zhou, Junliang
Cheng, Yongming
Xu, Hui
Yu, Xiaochen
author_facet Wang, Shuqing
Dong, Huanhuan
Wang, Meiqi
Ma, Wenbo
Cheng, Yue
Zhou, Junliang
Cheng, Yongming
Xu, Hui
Yu, Xiaochen
author_sort Wang, Shuqing
collection PubMed
description BACKGROUND: In recent years, carbapenem-resistant Klebsiella pneumoniae (CRKP) has emerged rapidly in China with the abuse and overuse of antibiotics, and infections caused by CRKP pose a serious threat to global public health safety. The present study aimed to explore the epidemiological characteristics of CRKP isolates in Northern China and to elucidate their drug resistance mechanisms. METHODS: 45 CRKP strains were consecutively collected at a teaching hospital from March 1st, 2018 to June 30th, 2018. Antimicrobial susceptibility was determined by the VITEK2 compact system and microbroth dilution method. Polymerase chain reaction (PCR) and sequencing were used to analyze multilocus sequence typing (MLST), drug resistance determinants, and plasmid types. The transfer of resistance genes was determined by conjugation. All statistical analysis was performed using SPSS 22.0 software. RESULTS: All 45 isolates showed multidrug resistance (MDR). MLST analysis showed ST11 (48.9%, 22/45) was the most frequent type. All of the 45 CRKP isolates contained carbapenemase genes, extended-spectrum β-lactamase (ESBL) genes, and plasmid-mediated quinolone resistance (PMQR) genes. For carbapenemase genes, KPC-2 (93.3%, 42/45) was the main genotype, and followed by GES (37.8%, 17/45) and NDM-1 (11.1%, 5/45). Plasmid typing analysis showed that IncFII and IncFIB were the most prevalent plasmids. The carbapenem resistance rate of K.pneumoniae was 11.4% and ICU was the main CRKP infection source. CONCLUSIONS: ST11 is the most frequent sequence type and KPC-2 is the predominant carbapenemase of CRKP strains in Northern China. KPC-2-ST11 are representative clonal lineages.
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spelling pubmed-97415282022-12-11 Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae in a Tertiary Hospital in Northern China Wang, Shuqing Dong, Huanhuan Wang, Meiqi Ma, Wenbo Cheng, Yue Zhou, Junliang Cheng, Yongming Xu, Hui Yu, Xiaochen Can J Infect Dis Med Microbiol Research Article BACKGROUND: In recent years, carbapenem-resistant Klebsiella pneumoniae (CRKP) has emerged rapidly in China with the abuse and overuse of antibiotics, and infections caused by CRKP pose a serious threat to global public health safety. The present study aimed to explore the epidemiological characteristics of CRKP isolates in Northern China and to elucidate their drug resistance mechanisms. METHODS: 45 CRKP strains were consecutively collected at a teaching hospital from March 1st, 2018 to June 30th, 2018. Antimicrobial susceptibility was determined by the VITEK2 compact system and microbroth dilution method. Polymerase chain reaction (PCR) and sequencing were used to analyze multilocus sequence typing (MLST), drug resistance determinants, and plasmid types. The transfer of resistance genes was determined by conjugation. All statistical analysis was performed using SPSS 22.0 software. RESULTS: All 45 isolates showed multidrug resistance (MDR). MLST analysis showed ST11 (48.9%, 22/45) was the most frequent type. All of the 45 CRKP isolates contained carbapenemase genes, extended-spectrum β-lactamase (ESBL) genes, and plasmid-mediated quinolone resistance (PMQR) genes. For carbapenemase genes, KPC-2 (93.3%, 42/45) was the main genotype, and followed by GES (37.8%, 17/45) and NDM-1 (11.1%, 5/45). Plasmid typing analysis showed that IncFII and IncFIB were the most prevalent plasmids. The carbapenem resistance rate of K.pneumoniae was 11.4% and ICU was the main CRKP infection source. CONCLUSIONS: ST11 is the most frequent sequence type and KPC-2 is the predominant carbapenemase of CRKP strains in Northern China. KPC-2-ST11 are representative clonal lineages. Hindawi 2022-12-03 /pmc/articles/PMC9741528/ /pubmed/36510603 http://dx.doi.org/10.1155/2022/2615753 Text en Copyright © 2022 Shuqing Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Shuqing
Dong, Huanhuan
Wang, Meiqi
Ma, Wenbo
Cheng, Yue
Zhou, Junliang
Cheng, Yongming
Xu, Hui
Yu, Xiaochen
Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae in a Tertiary Hospital in Northern China
title Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae in a Tertiary Hospital in Northern China
title_full Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae in a Tertiary Hospital in Northern China
title_fullStr Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae in a Tertiary Hospital in Northern China
title_full_unstemmed Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae in a Tertiary Hospital in Northern China
title_short Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae in a Tertiary Hospital in Northern China
title_sort molecular epidemiology of carbapenem-resistant klebsiella pneumoniae in a tertiary hospital in northern china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9741528/
https://www.ncbi.nlm.nih.gov/pubmed/36510603
http://dx.doi.org/10.1155/2022/2615753
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