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An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing
Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monos...
| Autores principales: | , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9741617/ https://www.ncbi.nlm.nih.gov/pubmed/36496505 http://dx.doi.org/10.1038/s41467-022-35355-6 |
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| author | Xiong, Feng Yang, Hong Song, Yi-Ge Qin, Hai-Bin Zhang, Qing-Yang Huang, Xian Jing, Wei Deng, Manfei Liu, Yang Liu, Zhixiang Shen, Yin Han, Yunyun Lu, Youming Xu, Xiangmin Holmes, Todd C. Luo, Minmin Zhao, Fei Luo, Min-Hua Zeng, Wen-Bo |
| author_facet | Xiong, Feng Yang, Hong Song, Yi-Ge Qin, Hai-Bin Zhang, Qing-Yang Huang, Xian Jing, Wei Deng, Manfei Liu, Yang Liu, Zhixiang Shen, Yin Han, Yunyun Lu, Youming Xu, Xiangmin Holmes, Todd C. Luo, Minmin Zhao, Fei Luo, Min-Hua Zeng, Wen-Bo |
| author_sort | Xiong, Feng |
| collection | PubMed |
| description | Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129(Amp) tracer system based on HSV-1 strain H129. The H129(Amp) tracer system consists of two components: an H129-dTK-T2-pac(Flox) helper which assists H129(Amp) tracer’s propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129(Amp) tracer. These improvements of H129(Amp) tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129(Amp) tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129(Amp) tracer system is a powerful tracing tool for revealing neuronal connectomes. |
| format | Online Article Text |
| id | pubmed-9741617 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-97416172022-12-12 An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing Xiong, Feng Yang, Hong Song, Yi-Ge Qin, Hai-Bin Zhang, Qing-Yang Huang, Xian Jing, Wei Deng, Manfei Liu, Yang Liu, Zhixiang Shen, Yin Han, Yunyun Lu, Youming Xu, Xiangmin Holmes, Todd C. Luo, Minmin Zhao, Fei Luo, Min-Hua Zeng, Wen-Bo Nat Commun Article Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129(Amp) tracer system based on HSV-1 strain H129. The H129(Amp) tracer system consists of two components: an H129-dTK-T2-pac(Flox) helper which assists H129(Amp) tracer’s propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129(Amp) tracer. These improvements of H129(Amp) tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129(Amp) tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129(Amp) tracer system is a powerful tracing tool for revealing neuronal connectomes. Nature Publishing Group UK 2022-12-10 /pmc/articles/PMC9741617/ /pubmed/36496505 http://dx.doi.org/10.1038/s41467-022-35355-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Article Xiong, Feng Yang, Hong Song, Yi-Ge Qin, Hai-Bin Zhang, Qing-Yang Huang, Xian Jing, Wei Deng, Manfei Liu, Yang Liu, Zhixiang Shen, Yin Han, Yunyun Lu, Youming Xu, Xiangmin Holmes, Todd C. Luo, Minmin Zhao, Fei Luo, Min-Hua Zeng, Wen-Bo An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing |
| title | An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing |
| title_full | An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing |
| title_fullStr | An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing |
| title_full_unstemmed | An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing |
| title_short | An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing |
| title_sort | hsv-1-h129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9741617/ https://www.ncbi.nlm.nih.gov/pubmed/36496505 http://dx.doi.org/10.1038/s41467-022-35355-6 |
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