Cargando…

Modulation of POPDC1 Expression by Phenothiazine and Trifluoperazine Suppress Colon Cancer Growth and Migration

OBJECTIVE: The aim of this study was to investigate the effects of CaM antagonist, PTZ, and TFP on cell proliferation and migration of colon cancer cells and its impact on POPDC protein expression. METHODS: The 50% inhibitory concentration (IC(50)) of PTZ and TFP in SW1116, SW480, HCT-15, and COLO20...

Descripción completa

Detalles Bibliográficos
Autores principales: Thomas, Fiona Macniesia, Sudi, Suhaini, Muhamad Salih, Falah Abbas, Palasuberniam, Praneetha, Suali, Latifah, Mohd Sani, Mohd Hijaz, Sunggip, Caroline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9741886/
https://www.ncbi.nlm.nih.gov/pubmed/36037145
http://dx.doi.org/10.31557/APJCP.2022.23.8.2863
_version_ 1784848411180138496
author Thomas, Fiona Macniesia
Sudi, Suhaini
Muhamad Salih, Falah Abbas
Palasuberniam, Praneetha
Suali, Latifah
Mohd Sani, Mohd Hijaz
Sunggip, Caroline
author_facet Thomas, Fiona Macniesia
Sudi, Suhaini
Muhamad Salih, Falah Abbas
Palasuberniam, Praneetha
Suali, Latifah
Mohd Sani, Mohd Hijaz
Sunggip, Caroline
author_sort Thomas, Fiona Macniesia
collection PubMed
description OBJECTIVE: The aim of this study was to investigate the effects of CaM antagonist, PTZ, and TFP on cell proliferation and migration of colon cancer cells and its impact on POPDC protein expression. METHODS: The 50% inhibitory concentration (IC(50)) of PTZ and TFP in SW1116, SW480, HCT-15, and COLO205 colon cancer cell lines are measured using MTT. Western blot and immunocytochemistry were used to determine the expression of PCNA, cyclin D1 (CD1), and POPDC proteins. Cell migration was observed using a scratch wound-healing assay. RESULTS: Treatment with PTZ and TFP inhibited colon cancer cells growth in a dose-dependent manner. PTZ and TFP significantly inhibited the activation of proliferation markers, PCNA and CD1, and the migration of colon cancer cells. Furthermore, POPDC protein was significantly suppressed in all cell types of colon cancer, particularly in SW480. Finally, the CaM antagonist upregulates the POPDC1 expression in colon cancer cells. CONCLUSION: These findings suggest that CaM antagonists suppress colon cancer cells proliferation via downregulation of CD1 and PCNA. In addition, POPDC protein could be used as a biomarker in colon cancer, and CaM antagonist could be used to regulate POPDC1 expression. This study suggests that targeting POPDC1 with CaM inhibition could be a potential therapeutic strategy for colon cancer treatment.
format Online
Article
Text
id pubmed-9741886
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher West Asia Organization for Cancer Prevention
record_format MEDLINE/PubMed
spelling pubmed-97418862022-12-16 Modulation of POPDC1 Expression by Phenothiazine and Trifluoperazine Suppress Colon Cancer Growth and Migration Thomas, Fiona Macniesia Sudi, Suhaini Muhamad Salih, Falah Abbas Palasuberniam, Praneetha Suali, Latifah Mohd Sani, Mohd Hijaz Sunggip, Caroline Asian Pac J Cancer Prev Research Article OBJECTIVE: The aim of this study was to investigate the effects of CaM antagonist, PTZ, and TFP on cell proliferation and migration of colon cancer cells and its impact on POPDC protein expression. METHODS: The 50% inhibitory concentration (IC(50)) of PTZ and TFP in SW1116, SW480, HCT-15, and COLO205 colon cancer cell lines are measured using MTT. Western blot and immunocytochemistry were used to determine the expression of PCNA, cyclin D1 (CD1), and POPDC proteins. Cell migration was observed using a scratch wound-healing assay. RESULTS: Treatment with PTZ and TFP inhibited colon cancer cells growth in a dose-dependent manner. PTZ and TFP significantly inhibited the activation of proliferation markers, PCNA and CD1, and the migration of colon cancer cells. Furthermore, POPDC protein was significantly suppressed in all cell types of colon cancer, particularly in SW480. Finally, the CaM antagonist upregulates the POPDC1 expression in colon cancer cells. CONCLUSION: These findings suggest that CaM antagonists suppress colon cancer cells proliferation via downregulation of CD1 and PCNA. In addition, POPDC protein could be used as a biomarker in colon cancer, and CaM antagonist could be used to regulate POPDC1 expression. This study suggests that targeting POPDC1 with CaM inhibition could be a potential therapeutic strategy for colon cancer treatment. West Asia Organization for Cancer Prevention 2022-08 /pmc/articles/PMC9741886/ /pubmed/36037145 http://dx.doi.org/10.31557/APJCP.2022.23.8.2863 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. https://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Research Article
Thomas, Fiona Macniesia
Sudi, Suhaini
Muhamad Salih, Falah Abbas
Palasuberniam, Praneetha
Suali, Latifah
Mohd Sani, Mohd Hijaz
Sunggip, Caroline
Modulation of POPDC1 Expression by Phenothiazine and Trifluoperazine Suppress Colon Cancer Growth and Migration
title Modulation of POPDC1 Expression by Phenothiazine and Trifluoperazine Suppress Colon Cancer Growth and Migration
title_full Modulation of POPDC1 Expression by Phenothiazine and Trifluoperazine Suppress Colon Cancer Growth and Migration
title_fullStr Modulation of POPDC1 Expression by Phenothiazine and Trifluoperazine Suppress Colon Cancer Growth and Migration
title_full_unstemmed Modulation of POPDC1 Expression by Phenothiazine and Trifluoperazine Suppress Colon Cancer Growth and Migration
title_short Modulation of POPDC1 Expression by Phenothiazine and Trifluoperazine Suppress Colon Cancer Growth and Migration
title_sort modulation of popdc1 expression by phenothiazine and trifluoperazine suppress colon cancer growth and migration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9741886/
https://www.ncbi.nlm.nih.gov/pubmed/36037145
http://dx.doi.org/10.31557/APJCP.2022.23.8.2863
work_keys_str_mv AT thomasfionamacniesia modulationofpopdc1expressionbyphenothiazineandtrifluoperazinesuppresscoloncancergrowthandmigration
AT sudisuhaini modulationofpopdc1expressionbyphenothiazineandtrifluoperazinesuppresscoloncancergrowthandmigration
AT muhamadsalihfalahabbas modulationofpopdc1expressionbyphenothiazineandtrifluoperazinesuppresscoloncancergrowthandmigration
AT palasuberniampraneetha modulationofpopdc1expressionbyphenothiazineandtrifluoperazinesuppresscoloncancergrowthandmigration
AT sualilatifah modulationofpopdc1expressionbyphenothiazineandtrifluoperazinesuppresscoloncancergrowthandmigration
AT mohdsanimohdhijaz modulationofpopdc1expressionbyphenothiazineandtrifluoperazinesuppresscoloncancergrowthandmigration
AT sunggipcaroline modulationofpopdc1expressionbyphenothiazineandtrifluoperazinesuppresscoloncancergrowthandmigration