Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids

Advances are needed in the site-directed mutagenesis of large plasmids for protein structure-function studies, as current methods are often inefficient, complicated and time-consuming. Here two new methods are reported that overcome these difficulties, namely the single primer extension reaction (SS...

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Detalles Bibliográficos
Autores principales: Jia, Huiyong, Couto-Rodriguez, Ricardo, Johnson, Sharon, Medina, Stephanie, Novillo, Brianna, Huynh, Peter, Kim, Matt, Cooper, Cade, Michalik, Meagan, Siew, Benjamin, Turesson, Elise, Maupin-Furlow, Julie A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742187/
https://www.ncbi.nlm.nih.gov/pubmed/36007808
http://dx.doi.org/10.1016/j.nbt.2022.08.004

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