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Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls
Melatonin, a multi-regulatory molecule, stimulates root generation and regulates many aspects of plant growth and developmental processes. To gain insight into the effects of melatonin on adventitious root (AR) formation, we use cucumber seedings subjected to one of three treatments: EW (hypocotyl e...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742233/ https://www.ncbi.nlm.nih.gov/pubmed/36518515 http://dx.doi.org/10.3389/fpls.2022.1059482 |
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author | Wang, Yuping Zhao, Hailiang Hu, Xiaohui Zhang, Yi Zhang, Zicun Zhang, Lu Li, Lixia Hou, Leiping Li, Meilan |
author_facet | Wang, Yuping Zhao, Hailiang Hu, Xiaohui Zhang, Yi Zhang, Zicun Zhang, Lu Li, Lixia Hou, Leiping Li, Meilan |
author_sort | Wang, Yuping |
collection | PubMed |
description | Melatonin, a multi-regulatory molecule, stimulates root generation and regulates many aspects of plant growth and developmental processes. To gain insight into the effects of melatonin on adventitious root (AR) formation, we use cucumber seedings subjected to one of three treatments: EW (hypocotyl exposed and irrigated with water), SW (hypocotyl shaded and irrigated with water) and SM (hypocotyl shaded and irrigated with 100 µM melatonin). Under shaded conditions, melatonin induced significant AR formation in the hypocotyl. To explore the mechanism of this melatonin-induced AR formation, we used transcriptome analysis to identify 1296 significant differentially expressed genes (DEGs). Comparing SM with SW, a total of 774 genes were upregulated and 522 genes were downregulated. The DEGs were classified among different metabolic pathways, especially those connected with the synthesis of secondary metabolites, with hormone signal transduction and with plant-pathogen interactions. Analyses indicate exogenous melatonin increased contents of endogenous auxin, jasmonic acid, salicylic acid, cytokinin and abscisic acid levels during AR formation. This study indicates melatonin promotes AR formation in cucumber seedings by regulating the expressions of genes related to hormone synthesis, signaling and cell wall formation, as well as by increasing the contents of auxin, cytokinin, jasmonic acid, salicylic acid and abscisic acid. This research elucidates the molecular mechanisms of melatonin’s role in promoting AR formation in the hypocotyl of cucumber seedings under shaded conditions. |
format | Online Article Text |
id | pubmed-9742233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97422332022-12-13 Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls Wang, Yuping Zhao, Hailiang Hu, Xiaohui Zhang, Yi Zhang, Zicun Zhang, Lu Li, Lixia Hou, Leiping Li, Meilan Front Plant Sci Plant Science Melatonin, a multi-regulatory molecule, stimulates root generation and regulates many aspects of plant growth and developmental processes. To gain insight into the effects of melatonin on adventitious root (AR) formation, we use cucumber seedings subjected to one of three treatments: EW (hypocotyl exposed and irrigated with water), SW (hypocotyl shaded and irrigated with water) and SM (hypocotyl shaded and irrigated with 100 µM melatonin). Under shaded conditions, melatonin induced significant AR formation in the hypocotyl. To explore the mechanism of this melatonin-induced AR formation, we used transcriptome analysis to identify 1296 significant differentially expressed genes (DEGs). Comparing SM with SW, a total of 774 genes were upregulated and 522 genes were downregulated. The DEGs were classified among different metabolic pathways, especially those connected with the synthesis of secondary metabolites, with hormone signal transduction and with plant-pathogen interactions. Analyses indicate exogenous melatonin increased contents of endogenous auxin, jasmonic acid, salicylic acid, cytokinin and abscisic acid levels during AR formation. This study indicates melatonin promotes AR formation in cucumber seedings by regulating the expressions of genes related to hormone synthesis, signaling and cell wall formation, as well as by increasing the contents of auxin, cytokinin, jasmonic acid, salicylic acid and abscisic acid. This research elucidates the molecular mechanisms of melatonin’s role in promoting AR formation in the hypocotyl of cucumber seedings under shaded conditions. Frontiers Media S.A. 2022-11-28 /pmc/articles/PMC9742233/ /pubmed/36518515 http://dx.doi.org/10.3389/fpls.2022.1059482 Text en Copyright © 2022 Wang, Zhao, Hu, Zhang, Zhang, Zhang, Li, Hou and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Wang, Yuping Zhao, Hailiang Hu, Xiaohui Zhang, Yi Zhang, Zicun Zhang, Lu Li, Lixia Hou, Leiping Li, Meilan Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls |
title | Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls |
title_full | Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls |
title_fullStr | Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls |
title_full_unstemmed | Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls |
title_short | Transcriptome and hormone Analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls |
title_sort | transcriptome and hormone analyses reveal that melatonin promotes adventitious rooting in shaded cucumber hypocotyls |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742233/ https://www.ncbi.nlm.nih.gov/pubmed/36518515 http://dx.doi.org/10.3389/fpls.2022.1059482 |
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