Cargando…
A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel
Due to its high biosafety, gellan gum (GG) hydrogel, a naturally occurring polysaccharide released by microorganisms, is frequently utilized in food and pharmaceuticals. In recent years, like GG, natural polysaccharide-based hydrogels have become increasingly popular in 3D-printed biomedical enginee...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742276/ https://www.ncbi.nlm.nih.gov/pubmed/36518197 http://dx.doi.org/10.3389/fbioe.2022.1070566 |
_version_ | 1784848491096309760 |
---|---|
author | Qi, Yong Zhang, Shuyun He, Yanni Ou, Shuanji Yang, Yang Qu, Yudun Li, Jiaxuan Lian, Wanmin Li, Guitao Tian, Junzhang Xu, Changpeng |
author_facet | Qi, Yong Zhang, Shuyun He, Yanni Ou, Shuanji Yang, Yang Qu, Yudun Li, Jiaxuan Lian, Wanmin Li, Guitao Tian, Junzhang Xu, Changpeng |
author_sort | Qi, Yong |
collection | PubMed |
description | Due to its high biosafety, gellan gum (GG) hydrogel, a naturally occurring polysaccharide released by microorganisms, is frequently utilized in food and pharmaceuticals. In recent years, like GG, natural polysaccharide-based hydrogels have become increasingly popular in 3D-printed biomedical engineering because of their simplicity of processing, considerable shear thinning characteristic, and minimal pH dependence. To mitigate the negative effects of the GG’s high biological inertia, poor cell adhesion, single cross-linked network, and high brittleness. Mesoporous silica nanospheres (MMSN) and Aldehyde-based methacrylated hyaluronic acid (AHAMA) were combined to sulfhydrated GG (TGG) to create a multi-network AHAMA/TGG/MMSN hydrogel in this study. For this composite hydrogel system, the multi-component offers several crosslinking networks: the double bond in AHAMA can be photocrosslinked by activating the photoinitiator, aldehyde groups on its side chain can create Schiff base bonds with MMSN, while TGG can self-curing at room temperature. The AHAMA/TGG/MMSN hydrogel, with a mass ratio of 2:6:1, exhibits good cell adhesion, high strength and elasticity, and great printability. We believe that this innovative multi-network hydrogel has potential uses in tissue regeneration and biomedical engineering. |
format | Online Article Text |
id | pubmed-9742276 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97422762022-12-13 A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel Qi, Yong Zhang, Shuyun He, Yanni Ou, Shuanji Yang, Yang Qu, Yudun Li, Jiaxuan Lian, Wanmin Li, Guitao Tian, Junzhang Xu, Changpeng Front Bioeng Biotechnol Bioengineering and Biotechnology Due to its high biosafety, gellan gum (GG) hydrogel, a naturally occurring polysaccharide released by microorganisms, is frequently utilized in food and pharmaceuticals. In recent years, like GG, natural polysaccharide-based hydrogels have become increasingly popular in 3D-printed biomedical engineering because of their simplicity of processing, considerable shear thinning characteristic, and minimal pH dependence. To mitigate the negative effects of the GG’s high biological inertia, poor cell adhesion, single cross-linked network, and high brittleness. Mesoporous silica nanospheres (MMSN) and Aldehyde-based methacrylated hyaluronic acid (AHAMA) were combined to sulfhydrated GG (TGG) to create a multi-network AHAMA/TGG/MMSN hydrogel in this study. For this composite hydrogel system, the multi-component offers several crosslinking networks: the double bond in AHAMA can be photocrosslinked by activating the photoinitiator, aldehyde groups on its side chain can create Schiff base bonds with MMSN, while TGG can self-curing at room temperature. The AHAMA/TGG/MMSN hydrogel, with a mass ratio of 2:6:1, exhibits good cell adhesion, high strength and elasticity, and great printability. We believe that this innovative multi-network hydrogel has potential uses in tissue regeneration and biomedical engineering. Frontiers Media S.A. 2022-11-28 /pmc/articles/PMC9742276/ /pubmed/36518197 http://dx.doi.org/10.3389/fbioe.2022.1070566 Text en Copyright © 2022 Qi, Zhang, He, Ou, Yang, Qu, Li, Lian, Li, Tian and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Qi, Yong Zhang, Shuyun He, Yanni Ou, Shuanji Yang, Yang Qu, Yudun Li, Jiaxuan Lian, Wanmin Li, Guitao Tian, Junzhang Xu, Changpeng A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel |
title | A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel |
title_full | A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel |
title_fullStr | A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel |
title_full_unstemmed | A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel |
title_short | A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel |
title_sort | cell adhesion-promoting multi-network 3d printing bio-ink based on natural polysaccharide hydrogel |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742276/ https://www.ncbi.nlm.nih.gov/pubmed/36518197 http://dx.doi.org/10.3389/fbioe.2022.1070566 |
work_keys_str_mv | AT qiyong acelladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT zhangshuyun acelladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT heyanni acelladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT oushuanji acelladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT yangyang acelladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT quyudun acelladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT lijiaxuan acelladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT lianwanmin acelladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT liguitao acelladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT tianjunzhang acelladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT xuchangpeng acelladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT qiyong celladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT zhangshuyun celladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT heyanni celladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT oushuanji celladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT yangyang celladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT quyudun celladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT lijiaxuan celladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT lianwanmin celladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT liguitao celladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT tianjunzhang celladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel AT xuchangpeng celladhesionpromotingmultinetwork3dprintingbioinkbasedonnaturalpolysaccharidehydrogel |