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A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel

Due to its high biosafety, gellan gum (GG) hydrogel, a naturally occurring polysaccharide released by microorganisms, is frequently utilized in food and pharmaceuticals. In recent years, like GG, natural polysaccharide-based hydrogels have become increasingly popular in 3D-printed biomedical enginee...

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Autores principales: Qi, Yong, Zhang, Shuyun, He, Yanni, Ou, Shuanji, Yang, Yang, Qu, Yudun, Li, Jiaxuan, Lian, Wanmin, Li, Guitao, Tian, Junzhang, Xu, Changpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742276/
https://www.ncbi.nlm.nih.gov/pubmed/36518197
http://dx.doi.org/10.3389/fbioe.2022.1070566
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author Qi, Yong
Zhang, Shuyun
He, Yanni
Ou, Shuanji
Yang, Yang
Qu, Yudun
Li, Jiaxuan
Lian, Wanmin
Li, Guitao
Tian, Junzhang
Xu, Changpeng
author_facet Qi, Yong
Zhang, Shuyun
He, Yanni
Ou, Shuanji
Yang, Yang
Qu, Yudun
Li, Jiaxuan
Lian, Wanmin
Li, Guitao
Tian, Junzhang
Xu, Changpeng
author_sort Qi, Yong
collection PubMed
description Due to its high biosafety, gellan gum (GG) hydrogel, a naturally occurring polysaccharide released by microorganisms, is frequently utilized in food and pharmaceuticals. In recent years, like GG, natural polysaccharide-based hydrogels have become increasingly popular in 3D-printed biomedical engineering because of their simplicity of processing, considerable shear thinning characteristic, and minimal pH dependence. To mitigate the negative effects of the GG’s high biological inertia, poor cell adhesion, single cross-linked network, and high brittleness. Mesoporous silica nanospheres (MMSN) and Aldehyde-based methacrylated hyaluronic acid (AHAMA) were combined to sulfhydrated GG (TGG) to create a multi-network AHAMA/TGG/MMSN hydrogel in this study. For this composite hydrogel system, the multi-component offers several crosslinking networks: the double bond in AHAMA can be photocrosslinked by activating the photoinitiator, aldehyde groups on its side chain can create Schiff base bonds with MMSN, while TGG can self-curing at room temperature. The AHAMA/TGG/MMSN hydrogel, with a mass ratio of 2:6:1, exhibits good cell adhesion, high strength and elasticity, and great printability. We believe that this innovative multi-network hydrogel has potential uses in tissue regeneration and biomedical engineering.
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spelling pubmed-97422762022-12-13 A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel Qi, Yong Zhang, Shuyun He, Yanni Ou, Shuanji Yang, Yang Qu, Yudun Li, Jiaxuan Lian, Wanmin Li, Guitao Tian, Junzhang Xu, Changpeng Front Bioeng Biotechnol Bioengineering and Biotechnology Due to its high biosafety, gellan gum (GG) hydrogel, a naturally occurring polysaccharide released by microorganisms, is frequently utilized in food and pharmaceuticals. In recent years, like GG, natural polysaccharide-based hydrogels have become increasingly popular in 3D-printed biomedical engineering because of their simplicity of processing, considerable shear thinning characteristic, and minimal pH dependence. To mitigate the negative effects of the GG’s high biological inertia, poor cell adhesion, single cross-linked network, and high brittleness. Mesoporous silica nanospheres (MMSN) and Aldehyde-based methacrylated hyaluronic acid (AHAMA) were combined to sulfhydrated GG (TGG) to create a multi-network AHAMA/TGG/MMSN hydrogel in this study. For this composite hydrogel system, the multi-component offers several crosslinking networks: the double bond in AHAMA can be photocrosslinked by activating the photoinitiator, aldehyde groups on its side chain can create Schiff base bonds with MMSN, while TGG can self-curing at room temperature. The AHAMA/TGG/MMSN hydrogel, with a mass ratio of 2:6:1, exhibits good cell adhesion, high strength and elasticity, and great printability. We believe that this innovative multi-network hydrogel has potential uses in tissue regeneration and biomedical engineering. Frontiers Media S.A. 2022-11-28 /pmc/articles/PMC9742276/ /pubmed/36518197 http://dx.doi.org/10.3389/fbioe.2022.1070566 Text en Copyright © 2022 Qi, Zhang, He, Ou, Yang, Qu, Li, Lian, Li, Tian and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Qi, Yong
Zhang, Shuyun
He, Yanni
Ou, Shuanji
Yang, Yang
Qu, Yudun
Li, Jiaxuan
Lian, Wanmin
Li, Guitao
Tian, Junzhang
Xu, Changpeng
A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel
title A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel
title_full A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel
title_fullStr A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel
title_full_unstemmed A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel
title_short A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel
title_sort cell adhesion-promoting multi-network 3d printing bio-ink based on natural polysaccharide hydrogel
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742276/
https://www.ncbi.nlm.nih.gov/pubmed/36518197
http://dx.doi.org/10.3389/fbioe.2022.1070566
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