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Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation
Root-associated bacteria strongly affect plant growth and development by synthesizing growth regulators and stress-relieving metabolites. The present study is mainly focused on assessing aerial root-associated bacteria of Rhynchostylis retusa (L.) Blume is an endemic epiphytic orchid responsible for...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742431/ https://www.ncbi.nlm.nih.gov/pubmed/36518501 http://dx.doi.org/10.3389/fpls.2022.1037109 |
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author | Yadav, Archana Mudoi, Kalpataru Dutta Kumar, Niraj Geed, Sachin Rameshrao Gogoi, Parishmita Sharma, Rabin K. Saikia, Ratul |
author_facet | Yadav, Archana Mudoi, Kalpataru Dutta Kumar, Niraj Geed, Sachin Rameshrao Gogoi, Parishmita Sharma, Rabin K. Saikia, Ratul |
author_sort | Yadav, Archana |
collection | PubMed |
description | Root-associated bacteria strongly affect plant growth and development by synthesizing growth regulators and stress-relieving metabolites. The present study is mainly focused on assessing aerial root-associated bacteria of Rhynchostylis retusa (L.) Blume is an endemic epiphytic orchid responsible for auxin production and influencing plant growth. A bacterial isolate, Microbacterium testaceum Y411, was found to be the most active producer of indole-3-acetic acid (IAA). The maximum IAA production (170µg/mL) was recorded with the bacterium at optimum process parameters such as pH 7, temperature 30°C, and tryptophan 1000 µg/mL in a culture medium for 48 h. The extracted auxin was purified and analyzed by FT-IR, HPLC, and HR-MS, indicating bacterial auxin has a similar mass value to 4-chloroindole-3-acetic acid auxin. Furthermore, the bacterial auxin was tested on in vitro propagation of orchid, Cymbidium aloifolium, and 90% seed germination was recorded in Murashige and Skoog’s medium supplemented with bacterial auxin. The novel results obtained in this study are used for agricultural applications and the Microbacterium testaceum Y411 is a valuable biotechnological resource for a natural auxin. |
format | Online Article Text |
id | pubmed-9742431 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97424312022-12-13 Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation Yadav, Archana Mudoi, Kalpataru Dutta Kumar, Niraj Geed, Sachin Rameshrao Gogoi, Parishmita Sharma, Rabin K. Saikia, Ratul Front Plant Sci Plant Science Root-associated bacteria strongly affect plant growth and development by synthesizing growth regulators and stress-relieving metabolites. The present study is mainly focused on assessing aerial root-associated bacteria of Rhynchostylis retusa (L.) Blume is an endemic epiphytic orchid responsible for auxin production and influencing plant growth. A bacterial isolate, Microbacterium testaceum Y411, was found to be the most active producer of indole-3-acetic acid (IAA). The maximum IAA production (170µg/mL) was recorded with the bacterium at optimum process parameters such as pH 7, temperature 30°C, and tryptophan 1000 µg/mL in a culture medium for 48 h. The extracted auxin was purified and analyzed by FT-IR, HPLC, and HR-MS, indicating bacterial auxin has a similar mass value to 4-chloroindole-3-acetic acid auxin. Furthermore, the bacterial auxin was tested on in vitro propagation of orchid, Cymbidium aloifolium, and 90% seed germination was recorded in Murashige and Skoog’s medium supplemented with bacterial auxin. The novel results obtained in this study are used for agricultural applications and the Microbacterium testaceum Y411 is a valuable biotechnological resource for a natural auxin. Frontiers Media S.A. 2022-11-28 /pmc/articles/PMC9742431/ /pubmed/36518501 http://dx.doi.org/10.3389/fpls.2022.1037109 Text en Copyright © 2022 Yadav, Mudoi, Kumar, Geed, Gogoi, Sharma and Saikia https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Yadav, Archana Mudoi, Kalpataru Dutta Kumar, Niraj Geed, Sachin Rameshrao Gogoi, Parishmita Sharma, Rabin K. Saikia, Ratul Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation |
title | Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation |
title_full | Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation |
title_fullStr | Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation |
title_full_unstemmed | Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation |
title_short | Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation |
title_sort | auxin biosynthesis by microbacterium testaceum y411 associated with orchid aerial roots and their efficacy in micropropagation |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742431/ https://www.ncbi.nlm.nih.gov/pubmed/36518501 http://dx.doi.org/10.3389/fpls.2022.1037109 |
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