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Physiological basis and differentially expressed genes in the salt tolerance mechanism of Thalassia hemprichii

Seagrass plays a vital role in the stability of marine ecology. The human development of marine resources has greatly affected the survival of seagrass. Seawater salinity is one of the important factors affecting its survival. Seagrass can survive in high saline environments for a long time and has...

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Autores principales: Shen, Jie, Wu, Zhongjie, Yin, Lei, Chen, Shiquan, Cai, Zefu, Geng, Xiaoxiao, Wang, Daoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742478/
https://www.ncbi.nlm.nih.gov/pubmed/36518512
http://dx.doi.org/10.3389/fpls.2022.975251
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author Shen, Jie
Wu, Zhongjie
Yin, Lei
Chen, Shiquan
Cai, Zefu
Geng, Xiaoxiao
Wang, Daoru
author_facet Shen, Jie
Wu, Zhongjie
Yin, Lei
Chen, Shiquan
Cai, Zefu
Geng, Xiaoxiao
Wang, Daoru
author_sort Shen, Jie
collection PubMed
description Seagrass plays a vital role in the stability of marine ecology. The human development of marine resources has greatly affected the survival of seagrass. Seawater salinity is one of the important factors affecting its survival. Seagrass can survive in high saline environments for a long time and has evolved a variety of effective tolerance mechanisms. However, little is known about the molecular mechanisms underlying salinity tolerance by seagrass. Thalassia hemprichii is a seagrass species with a global distribution. It is also an ecologically important plant species in coastal waters. Nevertheless, the continuous environmental deterioration has gradually reduced the ecological niche of seagrasses. In this study, experiments were conducted to examine the effects of salinity changes on T. hemprichii. The result showed that the optimal salinity for T. hemprichii is 25 to 35 PSU. Although it can survive under high and low salinity, high mortality rates are common in such environments. Further analyses revealed that high salinity induces growth and developmental retardation in T. hemprichii and further causes yellowing. The parenchyma cells in T. hemprichii also collapse, the structure changes, soluble sugar accumulates rapidly, soluble proteins accumulate rapidly, the malondialdehyde (MDA) content reduces, and lipid peroxidation reduces in plant membranes. The molecular mechanisms of salt tolerance differ significantly between marine and terrestrial plants. We found 319 differentially expressed genes (DEGs). These genes regulate transport and metabolism, promoting environmental adaptation. The expression of these genes changed rapidly upon exposure of T. hemprichii to salinity stress for three hours. This is the first report on the physiological and biochemical changes and gene expression regulation of T. hemprichii under different salinity conditions. The findings of this study well deepen our understanding of T. hemprichii adaptations to changes in the shoal living environment.
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spelling pubmed-97424782022-12-13 Physiological basis and differentially expressed genes in the salt tolerance mechanism of Thalassia hemprichii Shen, Jie Wu, Zhongjie Yin, Lei Chen, Shiquan Cai, Zefu Geng, Xiaoxiao Wang, Daoru Front Plant Sci Plant Science Seagrass plays a vital role in the stability of marine ecology. The human development of marine resources has greatly affected the survival of seagrass. Seawater salinity is one of the important factors affecting its survival. Seagrass can survive in high saline environments for a long time and has evolved a variety of effective tolerance mechanisms. However, little is known about the molecular mechanisms underlying salinity tolerance by seagrass. Thalassia hemprichii is a seagrass species with a global distribution. It is also an ecologically important plant species in coastal waters. Nevertheless, the continuous environmental deterioration has gradually reduced the ecological niche of seagrasses. In this study, experiments were conducted to examine the effects of salinity changes on T. hemprichii. The result showed that the optimal salinity for T. hemprichii is 25 to 35 PSU. Although it can survive under high and low salinity, high mortality rates are common in such environments. Further analyses revealed that high salinity induces growth and developmental retardation in T. hemprichii and further causes yellowing. The parenchyma cells in T. hemprichii also collapse, the structure changes, soluble sugar accumulates rapidly, soluble proteins accumulate rapidly, the malondialdehyde (MDA) content reduces, and lipid peroxidation reduces in plant membranes. The molecular mechanisms of salt tolerance differ significantly between marine and terrestrial plants. We found 319 differentially expressed genes (DEGs). These genes regulate transport and metabolism, promoting environmental adaptation. The expression of these genes changed rapidly upon exposure of T. hemprichii to salinity stress for three hours. This is the first report on the physiological and biochemical changes and gene expression regulation of T. hemprichii under different salinity conditions. The findings of this study well deepen our understanding of T. hemprichii adaptations to changes in the shoal living environment. Frontiers Media S.A. 2022-11-28 /pmc/articles/PMC9742478/ /pubmed/36518512 http://dx.doi.org/10.3389/fpls.2022.975251 Text en Copyright © 2022 Shen, Wu, Yin, Chen, Cai, Geng and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Shen, Jie
Wu, Zhongjie
Yin, Lei
Chen, Shiquan
Cai, Zefu
Geng, Xiaoxiao
Wang, Daoru
Physiological basis and differentially expressed genes in the salt tolerance mechanism of Thalassia hemprichii
title Physiological basis and differentially expressed genes in the salt tolerance mechanism of Thalassia hemprichii
title_full Physiological basis and differentially expressed genes in the salt tolerance mechanism of Thalassia hemprichii
title_fullStr Physiological basis and differentially expressed genes in the salt tolerance mechanism of Thalassia hemprichii
title_full_unstemmed Physiological basis and differentially expressed genes in the salt tolerance mechanism of Thalassia hemprichii
title_short Physiological basis and differentially expressed genes in the salt tolerance mechanism of Thalassia hemprichii
title_sort physiological basis and differentially expressed genes in the salt tolerance mechanism of thalassia hemprichii
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742478/
https://www.ncbi.nlm.nih.gov/pubmed/36518512
http://dx.doi.org/10.3389/fpls.2022.975251
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