In vitro evaluation of mutagenic, cytotoxic, genotoxic and oral irritation potential of nicotine pouch products

Non-clinical in vitro studies were conducted to investigate the characteristics of extracts from tobacco free nicotine pouches alongside a reference snus product and/or 1R6F reference cigarette. In vitro investigations were conducted in the Neutral Red Uptake (NRU) cytotoxicity assay, Bacterial Reve...

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Autores principales: Miller-Holt, Jacqueline, Baskerville-Abraham, Irene, Sakimura, Masanori, Fukushima, Toshiro, Puglisi, Andrea, Gafner, Jeremie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742906/
https://www.ncbi.nlm.nih.gov/pubmed/36518473
http://dx.doi.org/10.1016/j.toxrep.2022.06.008
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author Miller-Holt, Jacqueline
Baskerville-Abraham, Irene
Sakimura, Masanori
Fukushima, Toshiro
Puglisi, Andrea
Gafner, Jeremie
author_facet Miller-Holt, Jacqueline
Baskerville-Abraham, Irene
Sakimura, Masanori
Fukushima, Toshiro
Puglisi, Andrea
Gafner, Jeremie
author_sort Miller-Holt, Jacqueline
collection PubMed
description Non-clinical in vitro studies were conducted to investigate the characteristics of extracts from tobacco free nicotine pouches alongside a reference snus product and/or 1R6F reference cigarette. In vitro investigations were conducted in the Neutral Red Uptake (NRU) cytotoxicity assay, Bacterial Reverse Mutation (Ames) assay, and in vitro Mammalian Cell Micronucleus (ivMN) assay. These products were also investigated for their oral irritation potential in the EpiGingival™ 3D tissue model. Results from the Ames, in vitro Micronucleus and NRU assays indicated that the tested products were non-mutagenic, non-genotoxic and non-cytotoxic in contrast to results obtained for the 1R6F reference cigarette. Results from Complete Artificial Saliva (CAS) extracts from these products also failed to be classified as irritants (as measured using the MTT assay), in the EpiGingival™ 3D tissue model
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spelling pubmed-97429062022-12-13 In vitro evaluation of mutagenic, cytotoxic, genotoxic and oral irritation potential of nicotine pouch products Miller-Holt, Jacqueline Baskerville-Abraham, Irene Sakimura, Masanori Fukushima, Toshiro Puglisi, Andrea Gafner, Jeremie Toxicol Rep Regular Article Non-clinical in vitro studies were conducted to investigate the characteristics of extracts from tobacco free nicotine pouches alongside a reference snus product and/or 1R6F reference cigarette. In vitro investigations were conducted in the Neutral Red Uptake (NRU) cytotoxicity assay, Bacterial Reverse Mutation (Ames) assay, and in vitro Mammalian Cell Micronucleus (ivMN) assay. These products were also investigated for their oral irritation potential in the EpiGingival™ 3D tissue model. Results from the Ames, in vitro Micronucleus and NRU assays indicated that the tested products were non-mutagenic, non-genotoxic and non-cytotoxic in contrast to results obtained for the 1R6F reference cigarette. Results from Complete Artificial Saliva (CAS) extracts from these products also failed to be classified as irritants (as measured using the MTT assay), in the EpiGingival™ 3D tissue model Elsevier 2022-06-15 /pmc/articles/PMC9742906/ /pubmed/36518473 http://dx.doi.org/10.1016/j.toxrep.2022.06.008 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Regular Article
Miller-Holt, Jacqueline
Baskerville-Abraham, Irene
Sakimura, Masanori
Fukushima, Toshiro
Puglisi, Andrea
Gafner, Jeremie
In vitro evaluation of mutagenic, cytotoxic, genotoxic and oral irritation potential of nicotine pouch products
title In vitro evaluation of mutagenic, cytotoxic, genotoxic and oral irritation potential of nicotine pouch products
title_full In vitro evaluation of mutagenic, cytotoxic, genotoxic and oral irritation potential of nicotine pouch products
title_fullStr In vitro evaluation of mutagenic, cytotoxic, genotoxic and oral irritation potential of nicotine pouch products
title_full_unstemmed In vitro evaluation of mutagenic, cytotoxic, genotoxic and oral irritation potential of nicotine pouch products
title_short In vitro evaluation of mutagenic, cytotoxic, genotoxic and oral irritation potential of nicotine pouch products
title_sort in vitro evaluation of mutagenic, cytotoxic, genotoxic and oral irritation potential of nicotine pouch products
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742906/
https://www.ncbi.nlm.nih.gov/pubmed/36518473
http://dx.doi.org/10.1016/j.toxrep.2022.06.008
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