Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker

Widely used glyphosate-based herbicides can remain in agricultural fields or be spread into the surrounding environment. This study aimed to develop an antibody technique for assessing acetylcholinesterase (AChE) expression in Nile tilapia (Oreochromis niloticus) after glyphosate exposure in the ass...

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Autores principales: Nanthanawat, Phochit, Kiatprasert, Pongpat, Wattanakornsiri, Amnuay, Nanuam, Jakkaphun, Prasatkaew, Witchuda, Ruttanakorn, Sawipa, Thanomsit, Chutima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742946/
https://www.ncbi.nlm.nih.gov/pubmed/36518377
http://dx.doi.org/10.1016/j.toxrep.2022.07.009
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author Nanthanawat, Phochit
Kiatprasert, Pongpat
Wattanakornsiri, Amnuay
Nanuam, Jakkaphun
Prasatkaew, Witchuda
Ruttanakorn, Sawipa
Thanomsit, Chutima
author_facet Nanthanawat, Phochit
Kiatprasert, Pongpat
Wattanakornsiri, Amnuay
Nanuam, Jakkaphun
Prasatkaew, Witchuda
Ruttanakorn, Sawipa
Thanomsit, Chutima
author_sort Nanthanawat, Phochit
collection PubMed
description Widely used glyphosate-based herbicides can remain in agricultural fields or be spread into the surrounding environment. This study aimed to develop an antibody technique for assessing acetylcholinesterase (AChE) expression in Nile tilapia (Oreochromis niloticus) after glyphosate exposure in the assessed tissues consisting of plasma, muscle, gills, and liver. Results showed that the cumulative mortality of fish exposed to glyphosate increased with exposure time and glyphosate concentration. The LC(50) was evaluated using probit analysis. A sub-lethal concentration of 2 µL L(−1) glyphosate-based herbicide altered behavioural and physiological appearances. AChE expression decreased compared to that in the control group with increasing glyphosate exposure time. The 71 kDa AChE was consecutively expressed in plasma, muscle, gill, and liver under laboratory and field conditions, as detected by dot blot and Western blot analysis. Furthermore, laboratory and field studies of the gills showed positive immunohistochemical results. Although this study could detect AChE expression in many tissues, using gills to assess AChE exposure allowed the fish not to be sacrificed compared with other organ studies, and this technique can be used in both laboratory and field conditions. In conclusion, the antibody technique can be applied to measure AChE expression in the gill tissue to assess glyphosate-based herbicide exposure.
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spelling pubmed-97429462022-12-13 Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker Nanthanawat, Phochit Kiatprasert, Pongpat Wattanakornsiri, Amnuay Nanuam, Jakkaphun Prasatkaew, Witchuda Ruttanakorn, Sawipa Thanomsit, Chutima Toxicol Rep Regular Article Widely used glyphosate-based herbicides can remain in agricultural fields or be spread into the surrounding environment. This study aimed to develop an antibody technique for assessing acetylcholinesterase (AChE) expression in Nile tilapia (Oreochromis niloticus) after glyphosate exposure in the assessed tissues consisting of plasma, muscle, gills, and liver. Results showed that the cumulative mortality of fish exposed to glyphosate increased with exposure time and glyphosate concentration. The LC(50) was evaluated using probit analysis. A sub-lethal concentration of 2 µL L(−1) glyphosate-based herbicide altered behavioural and physiological appearances. AChE expression decreased compared to that in the control group with increasing glyphosate exposure time. The 71 kDa AChE was consecutively expressed in plasma, muscle, gill, and liver under laboratory and field conditions, as detected by dot blot and Western blot analysis. Furthermore, laboratory and field studies of the gills showed positive immunohistochemical results. Although this study could detect AChE expression in many tissues, using gills to assess AChE exposure allowed the fish not to be sacrificed compared with other organ studies, and this technique can be used in both laboratory and field conditions. In conclusion, the antibody technique can be applied to measure AChE expression in the gill tissue to assess glyphosate-based herbicide exposure. Elsevier 2022-07-13 /pmc/articles/PMC9742946/ /pubmed/36518377 http://dx.doi.org/10.1016/j.toxrep.2022.07.009 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Regular Article
Nanthanawat, Phochit
Kiatprasert, Pongpat
Wattanakornsiri, Amnuay
Nanuam, Jakkaphun
Prasatkaew, Witchuda
Ruttanakorn, Sawipa
Thanomsit, Chutima
Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker
title Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker
title_full Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker
title_fullStr Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker
title_full_unstemmed Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker
title_short Development of an antibody technique for acetylcholinesterase expression detection in the gill of Nile tilapia (Oreochromis niloticus) as a glyphosate-based herbicide biomarker
title_sort development of an antibody technique for acetylcholinesterase expression detection in the gill of nile tilapia (oreochromis niloticus) as a glyphosate-based herbicide biomarker
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742946/
https://www.ncbi.nlm.nih.gov/pubmed/36518377
http://dx.doi.org/10.1016/j.toxrep.2022.07.009
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