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Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis

Damnacanthal is an anthraquinone, extracted, and purified from the root of Morinda citrifolia in Thailand. This study aimed to measure acute oral toxicity and to investigate the anticancer activity of damnacanthal in colorectal tumorigenesis. We found that the growth of human colorectal cancer cells...

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Autores principales: Woradulayapinij, Warunya, Pothiluk, Apipu, Nualsanit, Thararat, Yimsoo, Thunyatorn, Yingmema, Werayut, Rojanapanthu, Pleumchitt, Hong, Yukyung, Baek, Seung Joon, Treesuppharat, Worapapar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742955/
https://www.ncbi.nlm.nih.gov/pubmed/36518435
http://dx.doi.org/10.1016/j.toxrep.2022.10.015
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author Woradulayapinij, Warunya
Pothiluk, Apipu
Nualsanit, Thararat
Yimsoo, Thunyatorn
Yingmema, Werayut
Rojanapanthu, Pleumchitt
Hong, Yukyung
Baek, Seung Joon
Treesuppharat, Worapapar
author_facet Woradulayapinij, Warunya
Pothiluk, Apipu
Nualsanit, Thararat
Yimsoo, Thunyatorn
Yingmema, Werayut
Rojanapanthu, Pleumchitt
Hong, Yukyung
Baek, Seung Joon
Treesuppharat, Worapapar
author_sort Woradulayapinij, Warunya
collection PubMed
description Damnacanthal is an anthraquinone, extracted, and purified from the root of Morinda citrifolia in Thailand. This study aimed to measure acute oral toxicity and to investigate the anticancer activity of damnacanthal in colorectal tumorigenesis. We found that the growth of human colorectal cancer cells was inhibited by damnacanthal in a dose- and a time-dependent manner. The growth inhibitory effect of damnacanthal was better than that of 5-FU used as a positive control in colorectal cancer cells, along with the downregulation of cell cycle protein cyclin D1. Similarly, an oral treatment of damnacanthal effectively inhibited the growth of colorectal tumor xenografts in nude mice, which was approximately 2–3-fold higher as compared to 5-FU by tumor size as well as expression of bioluminescence. Furthermore, the study of acute oral toxicity in mice exhibited a relatively low toxicity of damnacanthal with a LD(50) cut-off value of 2500 mg/kg according to OECD Guideline 423. These results reveal the potential therapeutic activity of a natural damnacanthal compound as an anti-colorectal cancer drug.
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spelling pubmed-97429552022-12-13 Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis Woradulayapinij, Warunya Pothiluk, Apipu Nualsanit, Thararat Yimsoo, Thunyatorn Yingmema, Werayut Rojanapanthu, Pleumchitt Hong, Yukyung Baek, Seung Joon Treesuppharat, Worapapar Toxicol Rep Regular Article Damnacanthal is an anthraquinone, extracted, and purified from the root of Morinda citrifolia in Thailand. This study aimed to measure acute oral toxicity and to investigate the anticancer activity of damnacanthal in colorectal tumorigenesis. We found that the growth of human colorectal cancer cells was inhibited by damnacanthal in a dose- and a time-dependent manner. The growth inhibitory effect of damnacanthal was better than that of 5-FU used as a positive control in colorectal cancer cells, along with the downregulation of cell cycle protein cyclin D1. Similarly, an oral treatment of damnacanthal effectively inhibited the growth of colorectal tumor xenografts in nude mice, which was approximately 2–3-fold higher as compared to 5-FU by tumor size as well as expression of bioluminescence. Furthermore, the study of acute oral toxicity in mice exhibited a relatively low toxicity of damnacanthal with a LD(50) cut-off value of 2500 mg/kg according to OECD Guideline 423. These results reveal the potential therapeutic activity of a natural damnacanthal compound as an anti-colorectal cancer drug. Elsevier 2022-10-29 /pmc/articles/PMC9742955/ /pubmed/36518435 http://dx.doi.org/10.1016/j.toxrep.2022.10.015 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Regular Article
Woradulayapinij, Warunya
Pothiluk, Apipu
Nualsanit, Thararat
Yimsoo, Thunyatorn
Yingmema, Werayut
Rojanapanthu, Pleumchitt
Hong, Yukyung
Baek, Seung Joon
Treesuppharat, Worapapar
Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis
title Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis
title_full Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis
title_fullStr Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis
title_full_unstemmed Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis
title_short Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis
title_sort acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742955/
https://www.ncbi.nlm.nih.gov/pubmed/36518435
http://dx.doi.org/10.1016/j.toxrep.2022.10.015
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