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Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis
Damnacanthal is an anthraquinone, extracted, and purified from the root of Morinda citrifolia in Thailand. This study aimed to measure acute oral toxicity and to investigate the anticancer activity of damnacanthal in colorectal tumorigenesis. We found that the growth of human colorectal cancer cells...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742955/ https://www.ncbi.nlm.nih.gov/pubmed/36518435 http://dx.doi.org/10.1016/j.toxrep.2022.10.015 |
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author | Woradulayapinij, Warunya Pothiluk, Apipu Nualsanit, Thararat Yimsoo, Thunyatorn Yingmema, Werayut Rojanapanthu, Pleumchitt Hong, Yukyung Baek, Seung Joon Treesuppharat, Worapapar |
author_facet | Woradulayapinij, Warunya Pothiluk, Apipu Nualsanit, Thararat Yimsoo, Thunyatorn Yingmema, Werayut Rojanapanthu, Pleumchitt Hong, Yukyung Baek, Seung Joon Treesuppharat, Worapapar |
author_sort | Woradulayapinij, Warunya |
collection | PubMed |
description | Damnacanthal is an anthraquinone, extracted, and purified from the root of Morinda citrifolia in Thailand. This study aimed to measure acute oral toxicity and to investigate the anticancer activity of damnacanthal in colorectal tumorigenesis. We found that the growth of human colorectal cancer cells was inhibited by damnacanthal in a dose- and a time-dependent manner. The growth inhibitory effect of damnacanthal was better than that of 5-FU used as a positive control in colorectal cancer cells, along with the downregulation of cell cycle protein cyclin D1. Similarly, an oral treatment of damnacanthal effectively inhibited the growth of colorectal tumor xenografts in nude mice, which was approximately 2–3-fold higher as compared to 5-FU by tumor size as well as expression of bioluminescence. Furthermore, the study of acute oral toxicity in mice exhibited a relatively low toxicity of damnacanthal with a LD(50) cut-off value of 2500 mg/kg according to OECD Guideline 423. These results reveal the potential therapeutic activity of a natural damnacanthal compound as an anti-colorectal cancer drug. |
format | Online Article Text |
id | pubmed-9742955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-97429552022-12-13 Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis Woradulayapinij, Warunya Pothiluk, Apipu Nualsanit, Thararat Yimsoo, Thunyatorn Yingmema, Werayut Rojanapanthu, Pleumchitt Hong, Yukyung Baek, Seung Joon Treesuppharat, Worapapar Toxicol Rep Regular Article Damnacanthal is an anthraquinone, extracted, and purified from the root of Morinda citrifolia in Thailand. This study aimed to measure acute oral toxicity and to investigate the anticancer activity of damnacanthal in colorectal tumorigenesis. We found that the growth of human colorectal cancer cells was inhibited by damnacanthal in a dose- and a time-dependent manner. The growth inhibitory effect of damnacanthal was better than that of 5-FU used as a positive control in colorectal cancer cells, along with the downregulation of cell cycle protein cyclin D1. Similarly, an oral treatment of damnacanthal effectively inhibited the growth of colorectal tumor xenografts in nude mice, which was approximately 2–3-fold higher as compared to 5-FU by tumor size as well as expression of bioluminescence. Furthermore, the study of acute oral toxicity in mice exhibited a relatively low toxicity of damnacanthal with a LD(50) cut-off value of 2500 mg/kg according to OECD Guideline 423. These results reveal the potential therapeutic activity of a natural damnacanthal compound as an anti-colorectal cancer drug. Elsevier 2022-10-29 /pmc/articles/PMC9742955/ /pubmed/36518435 http://dx.doi.org/10.1016/j.toxrep.2022.10.015 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Regular Article Woradulayapinij, Warunya Pothiluk, Apipu Nualsanit, Thararat Yimsoo, Thunyatorn Yingmema, Werayut Rojanapanthu, Pleumchitt Hong, Yukyung Baek, Seung Joon Treesuppharat, Worapapar Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis |
title | Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis |
title_full | Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis |
title_fullStr | Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis |
title_full_unstemmed | Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis |
title_short | Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis |
title_sort | acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742955/ https://www.ncbi.nlm.nih.gov/pubmed/36518435 http://dx.doi.org/10.1016/j.toxrep.2022.10.015 |
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