Cargando…

Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module()

The rapid development associated with Next Generation Tobacco Products (NGTP) has necessitated the development of high throughput methodologies to test their genotoxic potential in vitro when compared to conventional cigarette smoke (CS). An assessment of two Vitrocell® Mammalian 6/48 exposure modul...

Descripción completa

Detalles Bibliográficos
Autores principales: Keyser, Brian M., Leverette, Robert, Hollings, Michael, Seymour, Adam, Weidman, Randy A., Bequette, Carlton J., Jordan, Kristen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742965/
https://www.ncbi.nlm.nih.gov/pubmed/36518380
http://dx.doi.org/10.1016/j.toxrep.2022.11.001
_version_ 1784848633373392896
author Keyser, Brian M.
Leverette, Robert
Hollings, Michael
Seymour, Adam
Weidman, Randy A.
Bequette, Carlton J.
Jordan, Kristen
author_facet Keyser, Brian M.
Leverette, Robert
Hollings, Michael
Seymour, Adam
Weidman, Randy A.
Bequette, Carlton J.
Jordan, Kristen
author_sort Keyser, Brian M.
collection PubMed
description The rapid development associated with Next Generation Tobacco Products (NGTP) has necessitated the development of high throughput methodologies to test their genotoxic potential in vitro when compared to conventional cigarette smoke (CS). An assessment of two Vitrocell® Mammalian 6/48 exposure modules in three independent experiments was made by comparing results from multiple dosimetric techniques applied to aerosol generated from 3R4F Kentucky Reference cigarettes, commercially available electronically heated tobacco product (eHTP) and Electronic Nicotine Delivery System (ENDS) using the Vitrocell® VC10®. Real-time aerosol particle concentration was assessed by means of light scattering photometers and expressed as area under the curve (∑AUC). Nicotine concentrations were determined analytically by LC/MS. Humectant amount and distribution was assessed for eHTP and ENDS by the quantification of free glycerol in a phosphate buffered saline (PBS) trap, whereas total particulate matter (TPM) was assessed in the 3R4F cigarettes by the fluorescence of the particulate at 485 nm in anhydrous dimethyl sulfoxide (DMSO) trap within the exposure. Dose was adjusted by means of the addition of ambient air to dilute the whole smoke/aerosol in L/min and sampled into the system at a rate of 5 mL/min. Dilution of CS ranged from 8.0 to 0.5 L/min and for the eHTP and ENDS ranged from 4 to 0 L/min (undiluted). Dosimetric analysis of the system showed good concordance within replicates (p-values ranged from p = 0.3762 to p = 0.8926) and showed that the Vitrocell® Mammalian 6/48 is a viable means for genotoxic assessment of aerosol generated from both conventional cigarettes and NGTP. Results demonstrate the need to tailor dosimetry approaches to different aerosols due to variations in the physio-chemical composition, with a multi-dosimetry approach recommended.
format Online
Article
Text
id pubmed-9742965
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-97429652022-12-13 Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module() Keyser, Brian M. Leverette, Robert Hollings, Michael Seymour, Adam Weidman, Randy A. Bequette, Carlton J. Jordan, Kristen Toxicol Rep Regular Article The rapid development associated with Next Generation Tobacco Products (NGTP) has necessitated the development of high throughput methodologies to test their genotoxic potential in vitro when compared to conventional cigarette smoke (CS). An assessment of two Vitrocell® Mammalian 6/48 exposure modules in three independent experiments was made by comparing results from multiple dosimetric techniques applied to aerosol generated from 3R4F Kentucky Reference cigarettes, commercially available electronically heated tobacco product (eHTP) and Electronic Nicotine Delivery System (ENDS) using the Vitrocell® VC10®. Real-time aerosol particle concentration was assessed by means of light scattering photometers and expressed as area under the curve (∑AUC). Nicotine concentrations were determined analytically by LC/MS. Humectant amount and distribution was assessed for eHTP and ENDS by the quantification of free glycerol in a phosphate buffered saline (PBS) trap, whereas total particulate matter (TPM) was assessed in the 3R4F cigarettes by the fluorescence of the particulate at 485 nm in anhydrous dimethyl sulfoxide (DMSO) trap within the exposure. Dose was adjusted by means of the addition of ambient air to dilute the whole smoke/aerosol in L/min and sampled into the system at a rate of 5 mL/min. Dilution of CS ranged from 8.0 to 0.5 L/min and for the eHTP and ENDS ranged from 4 to 0 L/min (undiluted). Dosimetric analysis of the system showed good concordance within replicates (p-values ranged from p = 0.3762 to p = 0.8926) and showed that the Vitrocell® Mammalian 6/48 is a viable means for genotoxic assessment of aerosol generated from both conventional cigarettes and NGTP. Results demonstrate the need to tailor dosimetry approaches to different aerosols due to variations in the physio-chemical composition, with a multi-dosimetry approach recommended. Elsevier 2022-11-04 /pmc/articles/PMC9742965/ /pubmed/36518380 http://dx.doi.org/10.1016/j.toxrep.2022.11.001 Text en © 2022 Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Regular Article
Keyser, Brian M.
Leverette, Robert
Hollings, Michael
Seymour, Adam
Weidman, Randy A.
Bequette, Carlton J.
Jordan, Kristen
Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module()
title Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module()
title_full Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module()
title_fullStr Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module()
title_full_unstemmed Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module()
title_short Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module()
title_sort characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the vitrocell® mammalian 6/48 exposure module()
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9742965/
https://www.ncbi.nlm.nih.gov/pubmed/36518380
http://dx.doi.org/10.1016/j.toxrep.2022.11.001
work_keys_str_mv AT keyserbrianm characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule
AT leveretterobert characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule
AT hollingsmichael characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule
AT seymouradam characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule
AT weidmanrandya characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule
AT bequettecarltonj characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule
AT jordankristen characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule