Cargando…

Efficient biosynthesis of pinosylvin from lignin-derived cinnamic acid by metabolic engineering of Escherichia coli

BACKGROUND: The conversion of lignin-derived aromatic monomers into valuable chemicals has promising potential to improve the economic competitiveness of biomass biorefineries. Pinosylvin is an attractive pharmaceutical with multiple promising biological activities. RESULTS: Herein, Escherichia coli...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Yueli, Zhang, Chen, Zou, Lihua, Zheng, Zhaojuan, Ouyang, Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9743564/
https://www.ncbi.nlm.nih.gov/pubmed/36503554
http://dx.doi.org/10.1186/s13068-022-02236-5
_version_ 1784848750340997120
author Hu, Yueli
Zhang, Chen
Zou, Lihua
Zheng, Zhaojuan
Ouyang, Jia
author_facet Hu, Yueli
Zhang, Chen
Zou, Lihua
Zheng, Zhaojuan
Ouyang, Jia
author_sort Hu, Yueli
collection PubMed
description BACKGROUND: The conversion of lignin-derived aromatic monomers into valuable chemicals has promising potential to improve the economic competitiveness of biomass biorefineries. Pinosylvin is an attractive pharmaceutical with multiple promising biological activities. RESULTS: Herein, Escherichia coli was engineered to convert the lignin-derived standard model monomer cinnamic acid into pinosylvin by introducing two novel enzymes from the wood plant: stilbene synthase from Pinus pinea (PpSTS) and 4-Coumarate-CoA ligase from Populus trichocarpa (Ptr4CL4). The expression of Ptr4CL4 drastically improved the production of pinosylvin (42.5 ± 1.1 mg/L), achieving values 15.7-fold higher than that of Ptr4CL5 (another 4-Coumarate-CoA ligase from Populus trichocarpa) in the absence of cerulenin. By adjusting the expression strategy, the optimized engineered strain produced pinosylvin at 153.7 ± 2.2 mg/L with an extremely high yield of 1.20 ± 0.02 mg/mg cinnamic acid in the presence of cerulenin, which is 83.9% ± 1.17 of the theoretical yield. This is the highest reported pinosylvin yield directly from cinnamic acid to date. CONCLUSION: Our work highlights the feasibility of microbial production of pinosylvin from cinnamic acid and paves the way for converting lignin-related aromatics to valuable chemicals. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-022-02236-5.
format Online
Article
Text
id pubmed-9743564
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-97435642022-12-13 Efficient biosynthesis of pinosylvin from lignin-derived cinnamic acid by metabolic engineering of Escherichia coli Hu, Yueli Zhang, Chen Zou, Lihua Zheng, Zhaojuan Ouyang, Jia Biotechnol Biofuels Bioprod Research BACKGROUND: The conversion of lignin-derived aromatic monomers into valuable chemicals has promising potential to improve the economic competitiveness of biomass biorefineries. Pinosylvin is an attractive pharmaceutical with multiple promising biological activities. RESULTS: Herein, Escherichia coli was engineered to convert the lignin-derived standard model monomer cinnamic acid into pinosylvin by introducing two novel enzymes from the wood plant: stilbene synthase from Pinus pinea (PpSTS) and 4-Coumarate-CoA ligase from Populus trichocarpa (Ptr4CL4). The expression of Ptr4CL4 drastically improved the production of pinosylvin (42.5 ± 1.1 mg/L), achieving values 15.7-fold higher than that of Ptr4CL5 (another 4-Coumarate-CoA ligase from Populus trichocarpa) in the absence of cerulenin. By adjusting the expression strategy, the optimized engineered strain produced pinosylvin at 153.7 ± 2.2 mg/L with an extremely high yield of 1.20 ± 0.02 mg/mg cinnamic acid in the presence of cerulenin, which is 83.9% ± 1.17 of the theoretical yield. This is the highest reported pinosylvin yield directly from cinnamic acid to date. CONCLUSION: Our work highlights the feasibility of microbial production of pinosylvin from cinnamic acid and paves the way for converting lignin-related aromatics to valuable chemicals. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-022-02236-5. BioMed Central 2022-12-12 /pmc/articles/PMC9743564/ /pubmed/36503554 http://dx.doi.org/10.1186/s13068-022-02236-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Hu, Yueli
Zhang, Chen
Zou, Lihua
Zheng, Zhaojuan
Ouyang, Jia
Efficient biosynthesis of pinosylvin from lignin-derived cinnamic acid by metabolic engineering of Escherichia coli
title Efficient biosynthesis of pinosylvin from lignin-derived cinnamic acid by metabolic engineering of Escherichia coli
title_full Efficient biosynthesis of pinosylvin from lignin-derived cinnamic acid by metabolic engineering of Escherichia coli
title_fullStr Efficient biosynthesis of pinosylvin from lignin-derived cinnamic acid by metabolic engineering of Escherichia coli
title_full_unstemmed Efficient biosynthesis of pinosylvin from lignin-derived cinnamic acid by metabolic engineering of Escherichia coli
title_short Efficient biosynthesis of pinosylvin from lignin-derived cinnamic acid by metabolic engineering of Escherichia coli
title_sort efficient biosynthesis of pinosylvin from lignin-derived cinnamic acid by metabolic engineering of escherichia coli
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9743564/
https://www.ncbi.nlm.nih.gov/pubmed/36503554
http://dx.doi.org/10.1186/s13068-022-02236-5
work_keys_str_mv AT huyueli efficientbiosynthesisofpinosylvinfromligninderivedcinnamicacidbymetabolicengineeringofescherichiacoli
AT zhangchen efficientbiosynthesisofpinosylvinfromligninderivedcinnamicacidbymetabolicengineeringofescherichiacoli
AT zoulihua efficientbiosynthesisofpinosylvinfromligninderivedcinnamicacidbymetabolicengineeringofescherichiacoli
AT zhengzhaojuan efficientbiosynthesisofpinosylvinfromligninderivedcinnamicacidbymetabolicengineeringofescherichiacoli
AT ouyangjia efficientbiosynthesisofpinosylvinfromligninderivedcinnamicacidbymetabolicengineeringofescherichiacoli