Cargando…

A simple and robust method for isolating and analyzing chromatin-bound RNAs in Arabidopsis

BACKGROUND: Chromatin-bound RNAs are the primary product of transcription that undergo on-chromatin processing such as capping, splicing, and polyadenylation. These processing steps then determine the fate of RNAs. Albeit its vital importance, a simple and robust method for isolating different fract...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhang, Qiqi, Zhao, Fengli, Wu, Zhe, Zhu, Danling
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9743689/ https://www.ncbi.nlm.nih.gov/pubmed/36510301 http://dx.doi.org/10.1186/s13007-022-00967-y

Ejemplares similares

Coupling of co-transcriptional splicing and 3’ end Pol II pausing during termination in Arabidopsis
por: Zhou, Sixian, et al.
Publicado: (2023)

A simple and efficient method for isolating small RNAs from different plant species
por: Rosas-Cárdenas, Flor de Fátima, et al.
Publicado: (2011)

The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana
por: Wagner, Lucas, et al.
Publicado: (2017)

HiCoP, a simple and robust method for detecting interactions of regulatory regions
por: Zhang, Yan, et al.
Publicado: (2020)

Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method
por: Wu, Fu-Hui, et al.
Publicado: (2009)

A fast, efficient chromatin immunoprecipitation method for studying protein-DNA binding in Arabidopsis mesophyll protoplasts
por: Lee, Jeong Hwan, et al.
Publicado: (2017)

A Simple Rank Product Approach for Analyzing Two Classes
por: Yang, Tae Young
Publicado: (2015)

A rapid and robust method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation
por: Harrison, Samuel J, et al.
Publicado: (2006)

A simple and robust HPLC method to analyze lutein in wheat
por: Wu, Bangbang, et al.
Publicado: (2022)

Protocol: A simple phenol-based method for 96-well extraction of high quality RNA from Arabidopsis
por: Box, Mathew S, et al.
Publicado: (2011)

A robust and cost-effective approach to sequence and analyze complete genomes of small RNA viruses
por: Dimitrov, Kiril M., et al.
Publicado: (2017)

JACOP: A simple and robust method for the automated classification of protein sequences with modular architecture
por: Sperisen, Peter, et al.
Publicado: (2005)

Poinsettia protoplasts - a simple, robust and efficient system for transient gene expression studies
por: Pitzschke, Andrea, et al.
Publicado: (2012)

A simple and robust method for connecting small-molecule drugs using gene-expression signatures
por: Zhang, Shu-Dong, et al.
Publicado: (2008)

Methods for grafting Arabidopsis thaliana and Eutrema salsugineum
por: Li, Yan, et al.
Publicado: (2019)

A modified method for enzymatic isolation of and subsequent wax extraction from Arabidopsis thaliana leaf cuticle
por: Vráblová, Martina, et al.
Publicado: (2020)

A simple and efficient method for isolating polymorphic microsatellites from cDNA
por: Yue, Gen Hua, et al.
Publicado: (2009)

A rapid, simple method for the genetic discrimination of intact Arabidopsis thaliana mutant seeds using metabolic profiling by direct analysis in real-time mass spectrometry
por: Kim, Suk Weon, et al.
Publicado: (2011)

A simple, rapid method to isolate salt glands for three-dimensional visualization, fluorescence imaging and cytological studies
por: Tan, Wee-Kee, et al.
Publicado: (2010)

Simple extraction methods that prevent the artifactual conversion of chlorophyll to chlorophyllide during pigment isolation from leaf samples
por: Hu, Xueyun, et al.
Publicado: (2013)

Statistical methods for analyzing immunosignatures
por: Brown, Justin R, et al.
Publicado: (2011)

A simplified but robust method for the isolation of avian and mammalian muscle satellite cells
por: Baquero-Perez, Belinda, et al.
Publicado: (2012)

Methods for analyzing cost effectiveness data from cluster randomized trials
por: Bachmann, Max O, et al.
Publicado: (2007)

A DNA-based real-time PCR assay for robust growth quantification of the bacterial pathogen Pseudomonas syringae on Arabidopsis thaliana
por: Ross, Annegret, et al.
Publicado: (2016)

Atraumatic Flexor tendon retrieval- a simple method
por: Ozturk, Muhammed Besir, et al.
Publicado: (2013)

Isolation of neuronal chromatin from brain tissue
por: Jiang, Yan, et al.
Publicado: (2008)

Telomere-specific chromatin capture using a pyrrole–imidazole polyamide probe for the identification of proteins and non-coding RNAs
por: Ide, Satoru, et al.
Publicado: (2021)

A rapid method for quantifying free and bound acetate based on alkylation and GC-MS analysis
por: Tumanov, Sergey, et al.
Publicado: (2016)

A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings
por: Liang, Ying, et al.
Publicado: (2017)

A simple in vitro method to measure autophosphorylation of protein kinases
por: Taylor, Isaiah, et al.
Publicado: (2013)

An improved method for preparing Agrobacterium cells that simplifies the Arabidopsis transformation protocol
por: Logemann, Elke, et al.
Publicado: (2006)

A rapid chemical method for lysing Arabidopsis cells for protein analysis
por: Tsugama, Daisuke, et al.
Publicado: (2011)

A method for detecting single mRNA molecules in Arabidopsis thaliana
por: Duncan, Susan, et al.
Publicado: (2016)

3D fluorescent in situ hybridization using Arabidopsis leaf cryosections and isolated nuclei
por: Tirichine, Leïla, et al.
Publicado: (2009)

Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis
por: Li, Shengben, et al.
Publicado: (2016)

Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two
por: Ordon, Jana, et al.
Publicado: (2023)

A rapid and cost-effective fluorescence detection in tube (FDIT) method to analyze protein phosphorylation
por: Jin, Xiao, et al.
Publicado: (2016)

A simple in vitro method for evaluating dendritic cell-based vaccinations
por: Pham, Phuc Van, et al.
Publicado: (2014)

Simple propagation method for resident macrophages by co-culture and subculture, and their isolation from various organs
por: Ogawa, Kazushige, et al.
Publicado: (2019)

Nonparametric intensity bounds for the delineation of spatial clusters
por: Oliveira, Fernando LP, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_2c631vov0vjc0kpm8k7e6s41lc