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Optimization of Culture Media for Ex vivo T-Cell Expansion for Adoptive T-Cell Therapy
BACKGROUND: Adoptive T-cell therapy is a promising treatment strategy for cancer immunotherapy. The ability of immunotherapy based on the adoptive cell transfer of genetically modified T cells to generate powerful clinical responses has been highlighted by recent clinical success. Techniques which a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9744083/ https://www.ncbi.nlm.nih.gov/pubmed/36518860 http://dx.doi.org/10.4103/abr.abr_349_21 |
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author | Rahimmanesh, Ilnaz Tavangar, Mehrsa Zahedi, Seyedeh Noushin Azizi, Yadollah Khanahmad Shahreza, Hossein |
author_facet | Rahimmanesh, Ilnaz Tavangar, Mehrsa Zahedi, Seyedeh Noushin Azizi, Yadollah Khanahmad Shahreza, Hossein |
author_sort | Rahimmanesh, Ilnaz |
collection | PubMed |
description | BACKGROUND: Adoptive T-cell therapy is a promising treatment strategy for cancer immunotherapy. The ability of immunotherapy based on the adoptive cell transfer of genetically modified T cells to generate powerful clinical responses has been highlighted by recent clinical success. Techniques which are used to expand large numbers of T cells from different sources are critical in adoptive cell therapy. In this study, we evaluated the expansion, proliferation, activation of T lymphocytes, in the presence of various concentrations of interleukin-2, phytohemagglutinin (PHA), and insulin. MATERIALS AND METHODS: The effect of different supplemented culture media on T cell expansion was evaluated using MTT assay. The expression level of the Ki-67 proliferation marker was evaluated by real-time polymerase chain reaction. In addition, flow cytometry analysis was performed to access T cell subpopulations. RESULTS: Our results showed that supplemented culture media with an optimized concentration of PHA and interleukin-2 increased total fold expansion of T cells up to 500-fold with approximately 90% cell viability over 7 days. The quantitative assessment of Ki-67 in expanded T cells showed a significant elevation of this proliferation marker. Flow cytometry was also used to assess the proportion of CD4+ and CD8+ cells, and the main expanded population was CD3+ CD8+ cells. CONCLUSIONS: Based on these findings, we introduced a low-cost and rapid method to support the efficient expansion of T cells for adoptive cell therapy and other in vivo experiments. |
format | Online Article Text |
id | pubmed-9744083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-97440832022-12-13 Optimization of Culture Media for Ex vivo T-Cell Expansion for Adoptive T-Cell Therapy Rahimmanesh, Ilnaz Tavangar, Mehrsa Zahedi, Seyedeh Noushin Azizi, Yadollah Khanahmad Shahreza, Hossein Adv Biomed Res Original Article BACKGROUND: Adoptive T-cell therapy is a promising treatment strategy for cancer immunotherapy. The ability of immunotherapy based on the adoptive cell transfer of genetically modified T cells to generate powerful clinical responses has been highlighted by recent clinical success. Techniques which are used to expand large numbers of T cells from different sources are critical in adoptive cell therapy. In this study, we evaluated the expansion, proliferation, activation of T lymphocytes, in the presence of various concentrations of interleukin-2, phytohemagglutinin (PHA), and insulin. MATERIALS AND METHODS: The effect of different supplemented culture media on T cell expansion was evaluated using MTT assay. The expression level of the Ki-67 proliferation marker was evaluated by real-time polymerase chain reaction. In addition, flow cytometry analysis was performed to access T cell subpopulations. RESULTS: Our results showed that supplemented culture media with an optimized concentration of PHA and interleukin-2 increased total fold expansion of T cells up to 500-fold with approximately 90% cell viability over 7 days. The quantitative assessment of Ki-67 in expanded T cells showed a significant elevation of this proliferation marker. Flow cytometry was also used to assess the proportion of CD4+ and CD8+ cells, and the main expanded population was CD3+ CD8+ cells. CONCLUSIONS: Based on these findings, we introduced a low-cost and rapid method to support the efficient expansion of T cells for adoptive cell therapy and other in vivo experiments. Wolters Kluwer - Medknow 2022-10-31 /pmc/articles/PMC9744083/ /pubmed/36518860 http://dx.doi.org/10.4103/abr.abr_349_21 Text en Copyright: © 2022 Advanced Biomedical Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Rahimmanesh, Ilnaz Tavangar, Mehrsa Zahedi, Seyedeh Noushin Azizi, Yadollah Khanahmad Shahreza, Hossein Optimization of Culture Media for Ex vivo T-Cell Expansion for Adoptive T-Cell Therapy |
title | Optimization of Culture Media for Ex vivo T-Cell Expansion for Adoptive T-Cell Therapy |
title_full | Optimization of Culture Media for Ex vivo T-Cell Expansion for Adoptive T-Cell Therapy |
title_fullStr | Optimization of Culture Media for Ex vivo T-Cell Expansion for Adoptive T-Cell Therapy |
title_full_unstemmed | Optimization of Culture Media for Ex vivo T-Cell Expansion for Adoptive T-Cell Therapy |
title_short | Optimization of Culture Media for Ex vivo T-Cell Expansion for Adoptive T-Cell Therapy |
title_sort | optimization of culture media for ex vivo t-cell expansion for adoptive t-cell therapy |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9744083/ https://www.ncbi.nlm.nih.gov/pubmed/36518860 http://dx.doi.org/10.4103/abr.abr_349_21 |
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