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Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye

Russian wildrye, Psathyrostachys junceus (Fisch.) Nevski, is widely distributed in the high latitude areas of Eurasia. It plays an important role in grassland ecosystem maintenance, as well as being a valuable palatable forage species for livestock and wildlife. Russian wildrye germplasm has rich ph...

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Autores principales: Gao, Zhiqi, Yun, Lan, Li, Zhen, Liu, Qiyu, Zhang, Chen, Ma, Yingmei, Shi, Fengling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9744169/
https://www.ncbi.nlm.nih.gov/pubmed/36518293
http://dx.doi.org/10.7717/peerj.14442
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author Gao, Zhiqi
Yun, Lan
Li, Zhen
Liu, Qiyu
Zhang, Chen
Ma, Yingmei
Shi, Fengling
author_facet Gao, Zhiqi
Yun, Lan
Li, Zhen
Liu, Qiyu
Zhang, Chen
Ma, Yingmei
Shi, Fengling
author_sort Gao, Zhiqi
collection PubMed
description Russian wildrye, Psathyrostachys junceus (Fisch.) Nevski, is widely distributed in the high latitude areas of Eurasia. It plays an important role in grassland ecosystem maintenance, as well as being a valuable palatable forage species for livestock and wildlife. Russian wildrye germplasm has rich phenotypic and genetic diversity and has potential for improvement through crossbreeding. In this study, fifteen Russian wildrye hybrid combinations were produced and one F1 population with 123 putative hybrids was obtained by crossing two individual plants with significant differences in nutritional characteristics and reproductive tiller number. Twelve phenotypic traits of the F1 population were measured for three consecutive years, and ten of the twelve traits were in line with the genetic characteristics of quantitative traits. Hybrid superiority was revealed among F1 hybrids in both nutritional and reproductive traits. One non-recurrent parent plant with the highest PCA-synthesis score was selected and used to make a backcross with the ‘BOZOISKY SELECT’ male parent, and 143 putative BC1 hybrids were obtained. Sixteen pairs of EST-SSR primers were randomly selected from polymorphic primers derived from different expressed tiller trait related genes. Three primer pairs that amplified both the paternal and maternal characteristic band were used to assess the purity of the F1 population, and three primer pairs (with one shared primer pair) were used to identify the BC1 population. The hybrid purity was 96.75% for the F1 population and 95.80% for the BC1 population, and the results were confirmed by self-fertility test through bagging isolation. The genetic similarity coefficients between the F1 progeny and the male parent ranged from 0.500 to 0.895, and those between the BC1 progeny and the male parent ranged from 0.667 to 0.939. A subset of individuals in the BC1 population had closer genetic distance to the recurrent parent, and genetic variation within the BC1 population decreased compared to the F1 population.
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spelling pubmed-97441692022-12-13 Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye Gao, Zhiqi Yun, Lan Li, Zhen Liu, Qiyu Zhang, Chen Ma, Yingmei Shi, Fengling PeerJ Agricultural Science Russian wildrye, Psathyrostachys junceus (Fisch.) Nevski, is widely distributed in the high latitude areas of Eurasia. It plays an important role in grassland ecosystem maintenance, as well as being a valuable palatable forage species for livestock and wildlife. Russian wildrye germplasm has rich phenotypic and genetic diversity and has potential for improvement through crossbreeding. In this study, fifteen Russian wildrye hybrid combinations were produced and one F1 population with 123 putative hybrids was obtained by crossing two individual plants with significant differences in nutritional characteristics and reproductive tiller number. Twelve phenotypic traits of the F1 population were measured for three consecutive years, and ten of the twelve traits were in line with the genetic characteristics of quantitative traits. Hybrid superiority was revealed among F1 hybrids in both nutritional and reproductive traits. One non-recurrent parent plant with the highest PCA-synthesis score was selected and used to make a backcross with the ‘BOZOISKY SELECT’ male parent, and 143 putative BC1 hybrids were obtained. Sixteen pairs of EST-SSR primers were randomly selected from polymorphic primers derived from different expressed tiller trait related genes. Three primer pairs that amplified both the paternal and maternal characteristic band were used to assess the purity of the F1 population, and three primer pairs (with one shared primer pair) were used to identify the BC1 population. The hybrid purity was 96.75% for the F1 population and 95.80% for the BC1 population, and the results were confirmed by self-fertility test through bagging isolation. The genetic similarity coefficients between the F1 progeny and the male parent ranged from 0.500 to 0.895, and those between the BC1 progeny and the male parent ranged from 0.667 to 0.939. A subset of individuals in the BC1 population had closer genetic distance to the recurrent parent, and genetic variation within the BC1 population decreased compared to the F1 population. PeerJ Inc. 2022-11-30 /pmc/articles/PMC9744169/ /pubmed/36518293 http://dx.doi.org/10.7717/peerj.14442 Text en © 2022 Gao et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Gao, Zhiqi
Yun, Lan
Li, Zhen
Liu, Qiyu
Zhang, Chen
Ma, Yingmei
Shi, Fengling
Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye
title Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye
title_full Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye
title_fullStr Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye
title_full_unstemmed Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye
title_short Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye
title_sort hybrid purity identification using est-ssr markers and heterosis analysis of quantitative traits of russian wildrye
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9744169/
https://www.ncbi.nlm.nih.gov/pubmed/36518293
http://dx.doi.org/10.7717/peerj.14442
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