Cargando…

Functional crosstalk between the cohesin loader and chromatin remodelers

The cohesin complex participates in many structural and functional aspects of genome organization. Cohesin recruitment onto chromosomes requires nucleosome-free DNA and the Scc2-Scc4 cohesin loader complex that catalyzes topological cohesin loading. Additionally, the cohesin loader facilitates promo...

Descripción completa

Detalles Bibliográficos
Autores principales: Muñoz, Sofía, Jones, Andrew, Bouchoux, Céline, Gilmore, Tegan, Patel, Harshil, Uhlmann, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9744909/
https://www.ncbi.nlm.nih.gov/pubmed/36509793
http://dx.doi.org/10.1038/s41467-022-35444-6
_version_ 1784849031774601216
author Muñoz, Sofía
Jones, Andrew
Bouchoux, Céline
Gilmore, Tegan
Patel, Harshil
Uhlmann, Frank
author_facet Muñoz, Sofía
Jones, Andrew
Bouchoux, Céline
Gilmore, Tegan
Patel, Harshil
Uhlmann, Frank
author_sort Muñoz, Sofía
collection PubMed
description The cohesin complex participates in many structural and functional aspects of genome organization. Cohesin recruitment onto chromosomes requires nucleosome-free DNA and the Scc2-Scc4 cohesin loader complex that catalyzes topological cohesin loading. Additionally, the cohesin loader facilitates promoter nucleosome clearance in a yet unknown way, and it recognizes chromatin receptors such as the RSC chromatin remodeler. Here, we explore the cohesin loader-RSC interaction. Amongst multi-pronged contacts by Scc2 and Scc4, we find that Scc4 contacts a conserved patch on the RSC ATPase motor module. The cohesin loader directly stimulates in vitro nucleosome sliding by RSC, providing an explanation how it facilitates promoter nucleosome clearance. Furthermore, we observe cohesin loader interactions with a wide range of chromatin remodelers. Our results provide mechanistic insight into how the cohesin loader recognizes, as well as influences, the chromatin landscape, with implications for our understanding of human developmental disorders including Cornelia de Lange and Coffin-Siris syndromes.
format Online
Article
Text
id pubmed-9744909
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-97449092022-12-14 Functional crosstalk between the cohesin loader and chromatin remodelers Muñoz, Sofía Jones, Andrew Bouchoux, Céline Gilmore, Tegan Patel, Harshil Uhlmann, Frank Nat Commun Article The cohesin complex participates in many structural and functional aspects of genome organization. Cohesin recruitment onto chromosomes requires nucleosome-free DNA and the Scc2-Scc4 cohesin loader complex that catalyzes topological cohesin loading. Additionally, the cohesin loader facilitates promoter nucleosome clearance in a yet unknown way, and it recognizes chromatin receptors such as the RSC chromatin remodeler. Here, we explore the cohesin loader-RSC interaction. Amongst multi-pronged contacts by Scc2 and Scc4, we find that Scc4 contacts a conserved patch on the RSC ATPase motor module. The cohesin loader directly stimulates in vitro nucleosome sliding by RSC, providing an explanation how it facilitates promoter nucleosome clearance. Furthermore, we observe cohesin loader interactions with a wide range of chromatin remodelers. Our results provide mechanistic insight into how the cohesin loader recognizes, as well as influences, the chromatin landscape, with implications for our understanding of human developmental disorders including Cornelia de Lange and Coffin-Siris syndromes. Nature Publishing Group UK 2022-12-13 /pmc/articles/PMC9744909/ /pubmed/36509793 http://dx.doi.org/10.1038/s41467-022-35444-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Muñoz, Sofía
Jones, Andrew
Bouchoux, Céline
Gilmore, Tegan
Patel, Harshil
Uhlmann, Frank
Functional crosstalk between the cohesin loader and chromatin remodelers
title Functional crosstalk between the cohesin loader and chromatin remodelers
title_full Functional crosstalk between the cohesin loader and chromatin remodelers
title_fullStr Functional crosstalk between the cohesin loader and chromatin remodelers
title_full_unstemmed Functional crosstalk between the cohesin loader and chromatin remodelers
title_short Functional crosstalk between the cohesin loader and chromatin remodelers
title_sort functional crosstalk between the cohesin loader and chromatin remodelers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9744909/
https://www.ncbi.nlm.nih.gov/pubmed/36509793
http://dx.doi.org/10.1038/s41467-022-35444-6
work_keys_str_mv AT munozsofia functionalcrosstalkbetweenthecohesinloaderandchromatinremodelers
AT jonesandrew functionalcrosstalkbetweenthecohesinloaderandchromatinremodelers
AT bouchouxceline functionalcrosstalkbetweenthecohesinloaderandchromatinremodelers
AT gilmoretegan functionalcrosstalkbetweenthecohesinloaderandchromatinremodelers
AT patelharshil functionalcrosstalkbetweenthecohesinloaderandchromatinremodelers
AT uhlmannfrank functionalcrosstalkbetweenthecohesinloaderandchromatinremodelers