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Evaluation of the transverse aortic constriction model in ICR and C57BL/6J mice
Transverse aortic constriction (TAC) is a frequently used model to investigate pressure overload-induced progressive heart failure (HF); however, there is considerable phenotypic variation among different mouse strains and even sub-strains. Moreover, less is known about the TAC model in ICR mice. Th...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745147/ https://www.ncbi.nlm.nih.gov/pubmed/36523549 http://dx.doi.org/10.3389/fphys.2022.1026884 |
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author | Huang, Mengying Yu, Lishuang Wang, Xiaoping Wang, Mingmin Li, Weili Tang, Jiayang Ling, Guanjing Wei, Xiaoqi Wang, Yong Wang, Wei Wu, Yan Lu, Linghui |
author_facet | Huang, Mengying Yu, Lishuang Wang, Xiaoping Wang, Mingmin Li, Weili Tang, Jiayang Ling, Guanjing Wei, Xiaoqi Wang, Yong Wang, Wei Wu, Yan Lu, Linghui |
author_sort | Huang, Mengying |
collection | PubMed |
description | Transverse aortic constriction (TAC) is a frequently used model to investigate pressure overload-induced progressive heart failure (HF); however, there is considerable phenotypic variation among different mouse strains and even sub-strains. Moreover, less is known about the TAC model in ICR mice. Therefore, to determine the suitability of the ICR strain for TAC-induced HF research, we compared the effects of TAC on ICR and C57BL/6J mice at one, two and four weeks post-TAC via echocardiography, organ index, morphology, and histology. At the end of the study, behavior and gene expression patterns were assessed, and overall survival was monitored. Compared to the sham-operated mice, ICR and C57BL/6J mice displayed hypertrophic phenotypes with a significant increase in ventricle wall thickness, heart weight and ratio, and cross-sectional area of cardiomyocytes after a 2-week TAC exposure. In addition, ICR mice developed reduced systolic function and severe lung congestion 4 weeks post-TAC, whereas C57BL/6J did not. Besides, ICR mice demonstrated comparable survival, similar gene expression alteration but severer fibrotic remodeling and poor behavioral performance compared to the C57BL/6J mice. Our data demonstrated that ICR was quite sensitive to TAC-induced heart failure and can be an ideal research tool to investigate mechanisms and drug intervention for pressure overload-induced HF. |
format | Online Article Text |
id | pubmed-9745147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97451472022-12-14 Evaluation of the transverse aortic constriction model in ICR and C57BL/6J mice Huang, Mengying Yu, Lishuang Wang, Xiaoping Wang, Mingmin Li, Weili Tang, Jiayang Ling, Guanjing Wei, Xiaoqi Wang, Yong Wang, Wei Wu, Yan Lu, Linghui Front Physiol Physiology Transverse aortic constriction (TAC) is a frequently used model to investigate pressure overload-induced progressive heart failure (HF); however, there is considerable phenotypic variation among different mouse strains and even sub-strains. Moreover, less is known about the TAC model in ICR mice. Therefore, to determine the suitability of the ICR strain for TAC-induced HF research, we compared the effects of TAC on ICR and C57BL/6J mice at one, two and four weeks post-TAC via echocardiography, organ index, morphology, and histology. At the end of the study, behavior and gene expression patterns were assessed, and overall survival was monitored. Compared to the sham-operated mice, ICR and C57BL/6J mice displayed hypertrophic phenotypes with a significant increase in ventricle wall thickness, heart weight and ratio, and cross-sectional area of cardiomyocytes after a 2-week TAC exposure. In addition, ICR mice developed reduced systolic function and severe lung congestion 4 weeks post-TAC, whereas C57BL/6J did not. Besides, ICR mice demonstrated comparable survival, similar gene expression alteration but severer fibrotic remodeling and poor behavioral performance compared to the C57BL/6J mice. Our data demonstrated that ICR was quite sensitive to TAC-induced heart failure and can be an ideal research tool to investigate mechanisms and drug intervention for pressure overload-induced HF. Frontiers Media S.A. 2022-11-29 /pmc/articles/PMC9745147/ /pubmed/36523549 http://dx.doi.org/10.3389/fphys.2022.1026884 Text en Copyright © 2022 Huang, Yu, Wang, Wang, Li, Tang, Ling, Wei, Wang, Wang, Wu and Lu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Huang, Mengying Yu, Lishuang Wang, Xiaoping Wang, Mingmin Li, Weili Tang, Jiayang Ling, Guanjing Wei, Xiaoqi Wang, Yong Wang, Wei Wu, Yan Lu, Linghui Evaluation of the transverse aortic constriction model in ICR and C57BL/6J mice |
title | Evaluation of the transverse aortic constriction model in ICR and C57BL/6J mice |
title_full | Evaluation of the transverse aortic constriction model in ICR and C57BL/6J mice |
title_fullStr | Evaluation of the transverse aortic constriction model in ICR and C57BL/6J mice |
title_full_unstemmed | Evaluation of the transverse aortic constriction model in ICR and C57BL/6J mice |
title_short | Evaluation of the transverse aortic constriction model in ICR and C57BL/6J mice |
title_sort | evaluation of the transverse aortic constriction model in icr and c57bl/6j mice |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745147/ https://www.ncbi.nlm.nih.gov/pubmed/36523549 http://dx.doi.org/10.3389/fphys.2022.1026884 |
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