Feiyanning formula modulates the molecular mechanism of osimertinib resistance in lung cancer by regulating the Wnt/β-catenin pathway

Feiyanning Formula (FYN), a Chinese herbal formula derived from summarized clinical experience, is proven to have anti-tumor effects in lung cancer patients. Osimertinib, a third-generation epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), can improve progression-free survival a...

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Autores principales: Sang, Shuliu, Sun, Chenbing, Ding, Rongzhen, Jiang, Jingjie, Han, Yang, Gan, Shanshan, Bi, Ling, Gong, Yabin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Pharmacology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745155/
https://www.ncbi.nlm.nih.gov/pubmed/36523489
http://dx.doi.org/10.3389/fphar.2022.1019451
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author Sang, Shuliu
Sun, Chenbing
Ding, Rongzhen
Jiang, Jingjie
Han, Yang
Gan, Shanshan
Bi, Ling
Gong, Yabin
author_facet Sang, Shuliu
Sun, Chenbing
Ding, Rongzhen
Jiang, Jingjie
Han, Yang
Gan, Shanshan
Bi, Ling
Gong, Yabin
author_sort Sang, Shuliu
collection PubMed
description Feiyanning Formula (FYN), a Chinese herbal formula derived from summarized clinical experience, is proven to have anti-tumor effects in lung cancer patients. Osimertinib, a third-generation epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), can improve progression-free survival and overall survival of patients but drug resistance is inevitable. The current study evaluated the effects of FYN in osimertinib-resistant HCC827OR and PC9OR cells. FYN preferentially inhibited the proliferation and migration of HCC827OR and PC9OR cells. Moreover, FYN and osimertinib exhibited synergistic inhibitory effects on proliferation and migration. Real-time qPCR (RT-qPCR) and western blotting results indicated that FYN downregulated gene and protein levels of GSK3β and SRFS1, which are enriched in the Wnt/β-catenin pathway. Besides, FYN inhibited tumor growth and exhibited synergistic effects with osimertinib in vivo. Collectively, the results suggested that FYN exerted an anti-osimertinib resistance effect via the Wnt/β-catenin pathway.
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spelling pubmed-97451552022-12-14 Feiyanning formula modulates the molecular mechanism of osimertinib resistance in lung cancer by regulating the Wnt/β-catenin pathway Sang, Shuliu Sun, Chenbing Ding, Rongzhen Jiang, Jingjie Han, Yang Gan, Shanshan Bi, Ling Gong, Yabin Front Pharmacol Pharmacology Feiyanning Formula (FYN), a Chinese herbal formula derived from summarized clinical experience, is proven to have anti-tumor effects in lung cancer patients. Osimertinib, a third-generation epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), can improve progression-free survival and overall survival of patients but drug resistance is inevitable. The current study evaluated the effects of FYN in osimertinib-resistant HCC827OR and PC9OR cells. FYN preferentially inhibited the proliferation and migration of HCC827OR and PC9OR cells. Moreover, FYN and osimertinib exhibited synergistic inhibitory effects on proliferation and migration. Real-time qPCR (RT-qPCR) and western blotting results indicated that FYN downregulated gene and protein levels of GSK3β and SRFS1, which are enriched in the Wnt/β-catenin pathway. Besides, FYN inhibited tumor growth and exhibited synergistic effects with osimertinib in vivo. Collectively, the results suggested that FYN exerted an anti-osimertinib resistance effect via the Wnt/β-catenin pathway. Frontiers Media S.A. 2022-11-29 /pmc/articles/PMC9745155/ /pubmed/36523489 http://dx.doi.org/10.3389/fphar.2022.1019451 Text en Copyright © 2022 Sang, Sun, Ding, Jiang, Han, Gan, Bi and Gong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Sang, Shuliu
Sun, Chenbing
Ding, Rongzhen
Jiang, Jingjie
Han, Yang
Gan, Shanshan
Bi, Ling
Gong, Yabin
Feiyanning formula modulates the molecular mechanism of osimertinib resistance in lung cancer by regulating the Wnt/β-catenin pathway
title Feiyanning formula modulates the molecular mechanism of osimertinib resistance in lung cancer by regulating the Wnt/β-catenin pathway
title_full Feiyanning formula modulates the molecular mechanism of osimertinib resistance in lung cancer by regulating the Wnt/β-catenin pathway
title_fullStr Feiyanning formula modulates the molecular mechanism of osimertinib resistance in lung cancer by regulating the Wnt/β-catenin pathway
title_full_unstemmed Feiyanning formula modulates the molecular mechanism of osimertinib resistance in lung cancer by regulating the Wnt/β-catenin pathway
title_short Feiyanning formula modulates the molecular mechanism of osimertinib resistance in lung cancer by regulating the Wnt/β-catenin pathway
title_sort feiyanning formula modulates the molecular mechanism of osimertinib resistance in lung cancer by regulating the wnt/β-catenin pathway
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745155/
https://www.ncbi.nlm.nih.gov/pubmed/36523489
http://dx.doi.org/10.3389/fphar.2022.1019451
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