Cargando…
Isolation, structural determination, and antiviral activities of metabolites from vanitaracin A-producing Talaromyces sp.
Vanitaracin A, an anti-hepatitis B virus polyketide, has been previously isolated from Talaromyces sp. In the present study, we searched for novel compounds in the culture broth obtained from a vanitaracin A-producing fungus under various conditions. Three novel compounds (vanitaracin C, vanitaraphi...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745706/ https://www.ncbi.nlm.nih.gov/pubmed/36513753 http://dx.doi.org/10.1038/s41429-022-00585-9 |
_version_ | 1784849206925590528 |
---|---|
author | Kamisuki, Shinji Shibasaki, Hisanobu Murakami, Hironobu Fujino, Kan Tsukuda, Senko Kojima, Ikumi Ashikawa, Koudai Kanno, Kazuki Ishikawa, Tomohiro Saito, Tatsuo Sugawara, Fumio Watashi, Koichi Kuramochi, Kouji |
author_facet | Kamisuki, Shinji Shibasaki, Hisanobu Murakami, Hironobu Fujino, Kan Tsukuda, Senko Kojima, Ikumi Ashikawa, Koudai Kanno, Kazuki Ishikawa, Tomohiro Saito, Tatsuo Sugawara, Fumio Watashi, Koichi Kuramochi, Kouji |
author_sort | Kamisuki, Shinji |
collection | PubMed |
description | Vanitaracin A, an anti-hepatitis B virus polyketide, has been previously isolated from Talaromyces sp. In the present study, we searched for novel compounds in the culture broth obtained from a vanitaracin A-producing fungus under various conditions. Three novel compounds (vanitaracin C, vanitaraphilone A, and 2-hydroxy-4-(hydroxymethyl)-6-methylbenzaldehyde) were isolated, and their structures were determined using spectroscopic methods (1D/2D NMR and MS). In addition, the antiviral spectrum of vanitaracin A was examined by measuring its antiviral activities against rabies virus, Borna disease virus 1, and bovine leukemia virus. This compound exhibited antiviral activity against bovine leukemia virus, which is the causative agent of enzootic bovine leukosis. The anti-bovine leukemia virus effects of other compounds isolated from the vanitaracin A-producing fungus, namely, vanitaracins B and C, vanitaraphilone A, and 2-hydroxy-4-(hydroxymethyl)-6-methylbenzaldehyde, were also evaluated. Vanitaracin B, vanitaraphilone A and 2-hydroxy-4-(hydroxymethyl)-6-methylbenzaldehyde were also found to exhibit activity against bovine leukemia virus. These findings reveal the broad-spectrum antiviral activity of the vanitaracin scaffold and suggest several candidates for the development of anti-bovine leukemia virus drugs. |
format | Online Article Text |
id | pubmed-9745706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-97457062022-12-13 Isolation, structural determination, and antiviral activities of metabolites from vanitaracin A-producing Talaromyces sp. Kamisuki, Shinji Shibasaki, Hisanobu Murakami, Hironobu Fujino, Kan Tsukuda, Senko Kojima, Ikumi Ashikawa, Koudai Kanno, Kazuki Ishikawa, Tomohiro Saito, Tatsuo Sugawara, Fumio Watashi, Koichi Kuramochi, Kouji J Antibiot (Tokyo) Article Vanitaracin A, an anti-hepatitis B virus polyketide, has been previously isolated from Talaromyces sp. In the present study, we searched for novel compounds in the culture broth obtained from a vanitaracin A-producing fungus under various conditions. Three novel compounds (vanitaracin C, vanitaraphilone A, and 2-hydroxy-4-(hydroxymethyl)-6-methylbenzaldehyde) were isolated, and their structures were determined using spectroscopic methods (1D/2D NMR and MS). In addition, the antiviral spectrum of vanitaracin A was examined by measuring its antiviral activities against rabies virus, Borna disease virus 1, and bovine leukemia virus. This compound exhibited antiviral activity against bovine leukemia virus, which is the causative agent of enzootic bovine leukosis. The anti-bovine leukemia virus effects of other compounds isolated from the vanitaracin A-producing fungus, namely, vanitaracins B and C, vanitaraphilone A, and 2-hydroxy-4-(hydroxymethyl)-6-methylbenzaldehyde, were also evaluated. Vanitaracin B, vanitaraphilone A and 2-hydroxy-4-(hydroxymethyl)-6-methylbenzaldehyde were also found to exhibit activity against bovine leukemia virus. These findings reveal the broad-spectrum antiviral activity of the vanitaracin scaffold and suggest several candidates for the development of anti-bovine leukemia virus drugs. Nature Publishing Group UK 2022-12-13 2023 /pmc/articles/PMC9745706/ /pubmed/36513753 http://dx.doi.org/10.1038/s41429-022-00585-9 Text en © The Author(s), under exclusive licence to the Japan Antibiotics Research Association 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Kamisuki, Shinji Shibasaki, Hisanobu Murakami, Hironobu Fujino, Kan Tsukuda, Senko Kojima, Ikumi Ashikawa, Koudai Kanno, Kazuki Ishikawa, Tomohiro Saito, Tatsuo Sugawara, Fumio Watashi, Koichi Kuramochi, Kouji Isolation, structural determination, and antiviral activities of metabolites from vanitaracin A-producing Talaromyces sp. |
title | Isolation, structural determination, and antiviral activities of metabolites from vanitaracin A-producing Talaromyces sp. |
title_full | Isolation, structural determination, and antiviral activities of metabolites from vanitaracin A-producing Talaromyces sp. |
title_fullStr | Isolation, structural determination, and antiviral activities of metabolites from vanitaracin A-producing Talaromyces sp. |
title_full_unstemmed | Isolation, structural determination, and antiviral activities of metabolites from vanitaracin A-producing Talaromyces sp. |
title_short | Isolation, structural determination, and antiviral activities of metabolites from vanitaracin A-producing Talaromyces sp. |
title_sort | isolation, structural determination, and antiviral activities of metabolites from vanitaracin a-producing talaromyces sp. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745706/ https://www.ncbi.nlm.nih.gov/pubmed/36513753 http://dx.doi.org/10.1038/s41429-022-00585-9 |
work_keys_str_mv | AT kamisukishinji isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT shibasakihisanobu isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT murakamihironobu isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT fujinokan isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT tsukudasenko isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT kojimaikumi isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT ashikawakoudai isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT kannokazuki isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT ishikawatomohiro isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT saitotatsuo isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT sugawarafumio isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT watashikoichi isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp AT kuramochikouji isolationstructuraldeterminationandantiviralactivitiesofmetabolitesfromvanitaracinaproducingtalaromycessp |