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Exploring the epitranscriptome by native RNA sequencing

Chemical RNA modifications, collectively referred to as the “epitranscriptome,” are essential players in fine-tuning gene expression. Our ability to analyze RNA modifications has improved rapidly in recent years, largely due to the advent of high-throughput sequencing methodologies, which typically...

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Detalles Bibliográficos
Autores principales: Begik, Oguzhan, Mattick, John S., Novoa, Eva Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745831/
https://www.ncbi.nlm.nih.gov/pubmed/36104106
http://dx.doi.org/10.1261/rna.079404.122
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author Begik, Oguzhan
Mattick, John S.
Novoa, Eva Maria
author_facet Begik, Oguzhan
Mattick, John S.
Novoa, Eva Maria
author_sort Begik, Oguzhan
collection PubMed
description Chemical RNA modifications, collectively referred to as the “epitranscriptome,” are essential players in fine-tuning gene expression. Our ability to analyze RNA modifications has improved rapidly in recent years, largely due to the advent of high-throughput sequencing methodologies, which typically consist of coupling modification-specific reagents, such as antibodies or enzymes, to next-generation sequencing. Recently, it also became possible to map RNA modifications directly by sequencing native RNAs using nanopore technologies, which has been applied for the detection of a number of RNA modifications, such as N6-methyladenosine (m(6)A), pseudouridine (Ψ), and inosine (I). However, the signal modulations caused by most RNA modifications are yet to be determined. A global effort is needed to determine the signatures of the full range of RNA modifications to avoid the technical biases that have so far limited our understanding of the epitranscriptome.
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spelling pubmed-97458312023-01-04 Exploring the epitranscriptome by native RNA sequencing Begik, Oguzhan Mattick, John S. Novoa, Eva Maria RNA Mini-Review Chemical RNA modifications, collectively referred to as the “epitranscriptome,” are essential players in fine-tuning gene expression. Our ability to analyze RNA modifications has improved rapidly in recent years, largely due to the advent of high-throughput sequencing methodologies, which typically consist of coupling modification-specific reagents, such as antibodies or enzymes, to next-generation sequencing. Recently, it also became possible to map RNA modifications directly by sequencing native RNAs using nanopore technologies, which has been applied for the detection of a number of RNA modifications, such as N6-methyladenosine (m(6)A), pseudouridine (Ψ), and inosine (I). However, the signal modulations caused by most RNA modifications are yet to be determined. A global effort is needed to determine the signatures of the full range of RNA modifications to avoid the technical biases that have so far limited our understanding of the epitranscriptome. Cold Spring Harbor Laboratory Press 2022-11 /pmc/articles/PMC9745831/ /pubmed/36104106 http://dx.doi.org/10.1261/rna.079404.122 Text en © 2022 Begik et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society https://creativecommons.org/licenses/by-nc/4.0/This article, published in RNA, is available undera Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Mini-Review
Begik, Oguzhan
Mattick, John S.
Novoa, Eva Maria
Exploring the epitranscriptome by native RNA sequencing
title Exploring the epitranscriptome by native RNA sequencing
title_full Exploring the epitranscriptome by native RNA sequencing
title_fullStr Exploring the epitranscriptome by native RNA sequencing
title_full_unstemmed Exploring the epitranscriptome by native RNA sequencing
title_short Exploring the epitranscriptome by native RNA sequencing
title_sort exploring the epitranscriptome by native rna sequencing
topic Mini-Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745831/
https://www.ncbi.nlm.nih.gov/pubmed/36104106
http://dx.doi.org/10.1261/rna.079404.122
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