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Exploring the epitranscriptome by native RNA sequencing
Chemical RNA modifications, collectively referred to as the “epitranscriptome,” are essential players in fine-tuning gene expression. Our ability to analyze RNA modifications has improved rapidly in recent years, largely due to the advent of high-throughput sequencing methodologies, which typically...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745831/ https://www.ncbi.nlm.nih.gov/pubmed/36104106 http://dx.doi.org/10.1261/rna.079404.122 |
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author | Begik, Oguzhan Mattick, John S. Novoa, Eva Maria |
author_facet | Begik, Oguzhan Mattick, John S. Novoa, Eva Maria |
author_sort | Begik, Oguzhan |
collection | PubMed |
description | Chemical RNA modifications, collectively referred to as the “epitranscriptome,” are essential players in fine-tuning gene expression. Our ability to analyze RNA modifications has improved rapidly in recent years, largely due to the advent of high-throughput sequencing methodologies, which typically consist of coupling modification-specific reagents, such as antibodies or enzymes, to next-generation sequencing. Recently, it also became possible to map RNA modifications directly by sequencing native RNAs using nanopore technologies, which has been applied for the detection of a number of RNA modifications, such as N6-methyladenosine (m(6)A), pseudouridine (Ψ), and inosine (I). However, the signal modulations caused by most RNA modifications are yet to be determined. A global effort is needed to determine the signatures of the full range of RNA modifications to avoid the technical biases that have so far limited our understanding of the epitranscriptome. |
format | Online Article Text |
id | pubmed-9745831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-97458312023-01-04 Exploring the epitranscriptome by native RNA sequencing Begik, Oguzhan Mattick, John S. Novoa, Eva Maria RNA Mini-Review Chemical RNA modifications, collectively referred to as the “epitranscriptome,” are essential players in fine-tuning gene expression. Our ability to analyze RNA modifications has improved rapidly in recent years, largely due to the advent of high-throughput sequencing methodologies, which typically consist of coupling modification-specific reagents, such as antibodies or enzymes, to next-generation sequencing. Recently, it also became possible to map RNA modifications directly by sequencing native RNAs using nanopore technologies, which has been applied for the detection of a number of RNA modifications, such as N6-methyladenosine (m(6)A), pseudouridine (Ψ), and inosine (I). However, the signal modulations caused by most RNA modifications are yet to be determined. A global effort is needed to determine the signatures of the full range of RNA modifications to avoid the technical biases that have so far limited our understanding of the epitranscriptome. Cold Spring Harbor Laboratory Press 2022-11 /pmc/articles/PMC9745831/ /pubmed/36104106 http://dx.doi.org/10.1261/rna.079404.122 Text en © 2022 Begik et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society https://creativecommons.org/licenses/by-nc/4.0/This article, published in RNA, is available undera Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Mini-Review Begik, Oguzhan Mattick, John S. Novoa, Eva Maria Exploring the epitranscriptome by native RNA sequencing |
title | Exploring the epitranscriptome by native RNA sequencing |
title_full | Exploring the epitranscriptome by native RNA sequencing |
title_fullStr | Exploring the epitranscriptome by native RNA sequencing |
title_full_unstemmed | Exploring the epitranscriptome by native RNA sequencing |
title_short | Exploring the epitranscriptome by native RNA sequencing |
title_sort | exploring the epitranscriptome by native rna sequencing |
topic | Mini-Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745831/ https://www.ncbi.nlm.nih.gov/pubmed/36104106 http://dx.doi.org/10.1261/rna.079404.122 |
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