Cargando…

Phenotypic and functional alterations of monocyte subsets with aging

BACKGROUND: It has been widely accepted that monocytes are one of the central mediators contributing to inflammaging. However, it remains unclear whether aged monocytes, similar to aged T cells, have characteristics of hyperactivation and increased expression of co-inhibitory molecules. METHODS: Per...

Descripción completa

Detalles Bibliográficos
Autores principales: Cao, Yu, Fan, Yang, Li, Fangyuan, Hao, Yu, Kong, Yaxian, Chen, Chen, Hao, Xing, Han, Dannuo, Li, Guoli, Wang, Zengtao, Song, Chuan, Han, Junyan, Zeng, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745938/
https://www.ncbi.nlm.nih.gov/pubmed/36514074
http://dx.doi.org/10.1186/s12979-022-00321-9
_version_ 1784849256359657472
author Cao, Yu
Fan, Yang
Li, Fangyuan
Hao, Yu
Kong, Yaxian
Chen, Chen
Hao, Xing
Han, Dannuo
Li, Guoli
Wang, Zengtao
Song, Chuan
Han, Junyan
Zeng, Hui
author_facet Cao, Yu
Fan, Yang
Li, Fangyuan
Hao, Yu
Kong, Yaxian
Chen, Chen
Hao, Xing
Han, Dannuo
Li, Guoli
Wang, Zengtao
Song, Chuan
Han, Junyan
Zeng, Hui
author_sort Cao, Yu
collection PubMed
description BACKGROUND: It has been widely accepted that monocytes are one of the central mediators contributing to inflammaging. However, it remains unclear whether aged monocytes, similar to aged T cells, have characteristics of hyperactivation and increased expression of co-inhibitory molecules. METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from young (21–40 years old), middle-aged (41–60 years old), and older human subjects (> 60 years old). Flow cytometry was used to monitor changes in the expression of surface molecules of monocyte subsets and cytokine-producing capacity. RESULTS: We observed increased tumor necrosis factor-α: TNF-α and decreased interleukin-6 (IL-6) production in monocytes from older adults compared with young and middle-aged adults. Older adults had a greater percentage of intermediate and non-classical monocyte subsets, along with increased levels of the immune activation markers human leukocyte antigen-DR (HLA-DR), and adhesion molecules cluster of differentiation molecule 11b (CD11b) and L-selectin (CD62L). Furthermore, we observed increased C–C motif chemokine receptor 2 (CCR2) expression on classical monocytes and decreased C-X3-C motif chemokine receptor 1 (CX3CR1) expression on non-classical monocytes in older adult subjects. The expression of co-inhibitory receptors was reduced on monocyte subsets in older adults. CONCLUSIONS: Circulating monocytes in older adults exhibit increased expression of activation, adhesion, and migration markers, but decreased expression of co-inhibitory molecules. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12979-022-00321-9.
format Online
Article
Text
id pubmed-9745938
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-97459382022-12-14 Phenotypic and functional alterations of monocyte subsets with aging Cao, Yu Fan, Yang Li, Fangyuan Hao, Yu Kong, Yaxian Chen, Chen Hao, Xing Han, Dannuo Li, Guoli Wang, Zengtao Song, Chuan Han, Junyan Zeng, Hui Immun Ageing Research BACKGROUND: It has been widely accepted that monocytes are one of the central mediators contributing to inflammaging. However, it remains unclear whether aged monocytes, similar to aged T cells, have characteristics of hyperactivation and increased expression of co-inhibitory molecules. METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from young (21–40 years old), middle-aged (41–60 years old), and older human subjects (> 60 years old). Flow cytometry was used to monitor changes in the expression of surface molecules of monocyte subsets and cytokine-producing capacity. RESULTS: We observed increased tumor necrosis factor-α: TNF-α and decreased interleukin-6 (IL-6) production in monocytes from older adults compared with young and middle-aged adults. Older adults had a greater percentage of intermediate and non-classical monocyte subsets, along with increased levels of the immune activation markers human leukocyte antigen-DR (HLA-DR), and adhesion molecules cluster of differentiation molecule 11b (CD11b) and L-selectin (CD62L). Furthermore, we observed increased C–C motif chemokine receptor 2 (CCR2) expression on classical monocytes and decreased C-X3-C motif chemokine receptor 1 (CX3CR1) expression on non-classical monocytes in older adult subjects. The expression of co-inhibitory receptors was reduced on monocyte subsets in older adults. CONCLUSIONS: Circulating monocytes in older adults exhibit increased expression of activation, adhesion, and migration markers, but decreased expression of co-inhibitory molecules. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12979-022-00321-9. BioMed Central 2022-12-13 /pmc/articles/PMC9745938/ /pubmed/36514074 http://dx.doi.org/10.1186/s12979-022-00321-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Cao, Yu
Fan, Yang
Li, Fangyuan
Hao, Yu
Kong, Yaxian
Chen, Chen
Hao, Xing
Han, Dannuo
Li, Guoli
Wang, Zengtao
Song, Chuan
Han, Junyan
Zeng, Hui
Phenotypic and functional alterations of monocyte subsets with aging
title Phenotypic and functional alterations of monocyte subsets with aging
title_full Phenotypic and functional alterations of monocyte subsets with aging
title_fullStr Phenotypic and functional alterations of monocyte subsets with aging
title_full_unstemmed Phenotypic and functional alterations of monocyte subsets with aging
title_short Phenotypic and functional alterations of monocyte subsets with aging
title_sort phenotypic and functional alterations of monocyte subsets with aging
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745938/
https://www.ncbi.nlm.nih.gov/pubmed/36514074
http://dx.doi.org/10.1186/s12979-022-00321-9
work_keys_str_mv AT caoyu phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT fanyang phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT lifangyuan phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT haoyu phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT kongyaxian phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT chenchen phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT haoxing phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT handannuo phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT liguoli phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT wangzengtao phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT songchuan phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT hanjunyan phenotypicandfunctionalalterationsofmonocytesubsetswithaging
AT zenghui phenotypicandfunctionalalterationsofmonocytesubsetswithaging