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Changes in soil properties and the phoD-harboring bacteria of the alfalfa field in response to phosphite treatment

Phosphite, a reduced form of orthophosphate, is characterized by high solubility, and transportation efficiency and can be used as potential phosphorus fertilizer, plant biostimulant and supplemental fertilizer in agriculture. However, the effects of phosphite fertilizer on soil properties and micro...

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Autores principales: Li, Zhenyi, Wang, Jixiang, Wu, Yao, Hu, Jingyun, Cong, Lili, Yang, Chao, Fu, Jinmin, Sun, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746240/
https://www.ncbi.nlm.nih.gov/pubmed/36523837
http://dx.doi.org/10.3389/fmicb.2022.1013896
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author Li, Zhenyi
Wang, Jixiang
Wu, Yao
Hu, Jingyun
Cong, Lili
Yang, Chao
Fu, Jinmin
Sun, Juan
author_facet Li, Zhenyi
Wang, Jixiang
Wu, Yao
Hu, Jingyun
Cong, Lili
Yang, Chao
Fu, Jinmin
Sun, Juan
author_sort Li, Zhenyi
collection PubMed
description Phosphite, a reduced form of orthophosphate, is characterized by high solubility, and transportation efficiency and can be used as potential phosphorus fertilizer, plant biostimulant and supplemental fertilizer in agriculture. However, the effects of phosphite fertilizer on soil properties and microorganisms are poorly understood. This study evaluated the effects of phosphate and phosphite fertilizers on the different forms of phosphorus, alkaline phosphatase (ALP) activity, and phoD-harboring bacterial community in the alfalfa (Medicago sativa) field. The study used four concentrations (30, 60, 90, and 120 mg P(2)O(5) kg(−1) soil) of phosphate (KH(2)PO(4)) and phosphite (KH(2)PO(3)) fertilizers for the alfalfa field treatment. The results showed that both phosphite and phosphate fertilizers increased the total phosphorus (TP) and available phosphorus (AP) contents in the soil. The phosphorus content of the phosphite-treated soil was lower than that of the phosphate-treated one. TP, inorganic phosphate (Pi), and AP negatively regulated ALP activity, which decreased with increasing phosphate and phosphite fertilizers concentrations. Furthermore, high-throughput sequencing analysis identified 6 phyla and 29 families, which were classified from the altered operational taxonomic units (OTUs) of the soil samples. The redundancy analysis (RDA) revealed that pH, TP, AP and Pi were significantly related to the phoD-harboring bacterial community constructure. The different fertilizer treatments altered the key families, contributing to soil ALP activities. Frankiaceae, Sphingomonadaceae, and Rhizobiaceae positively correlated with ALP activity in phosphite-treated soil. Moreover, the structural equation model (SEM) revealed that ALP activity was affected by the phoD-harboring bacterial community through altered organic phosphorus (Po), AP, total nitrogen (TN), soil organic carbon (SOC), and pH levels under phosphate fertilizer treatment. However, the effect was achieved through positive regulation of pH and AP under phosphite fertilizer. Thus, the changes in soil properties and phoD-harboring bacteria in response to phosphate and phosphite treatments differed in the alfalfa field. This study is the first to report the effects of phosphite on the soil properties of an alfalfa field and provides a strong basis for phosphite utilization in the future. HIGHLIGHTS: – Phosphite and phosphate increase the total phosphorus and available phosphorus. – The pH was the dominant factor influencing the phoD-harboring bacterial community under phosphite fertilizer. – The response of soil properties and phoD-harboring bacterial community to phosphate and phosphite fertilizers differed in the alfalfa field.
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spelling pubmed-97462402022-12-14 Changes in soil properties and the phoD-harboring bacteria of the alfalfa field in response to phosphite treatment Li, Zhenyi Wang, Jixiang Wu, Yao Hu, Jingyun Cong, Lili Yang, Chao Fu, Jinmin Sun, Juan Front Microbiol Microbiology Phosphite, a reduced form of orthophosphate, is characterized by high solubility, and transportation efficiency and can be used as potential phosphorus fertilizer, plant biostimulant and supplemental fertilizer in agriculture. However, the effects of phosphite fertilizer on soil properties and microorganisms are poorly understood. This study evaluated the effects of phosphate and phosphite fertilizers on the different forms of phosphorus, alkaline phosphatase (ALP) activity, and phoD-harboring bacterial community in the alfalfa (Medicago sativa) field. The study used four concentrations (30, 60, 90, and 120 mg P(2)O(5) kg(−1) soil) of phosphate (KH(2)PO(4)) and phosphite (KH(2)PO(3)) fertilizers for the alfalfa field treatment. The results showed that both phosphite and phosphate fertilizers increased the total phosphorus (TP) and available phosphorus (AP) contents in the soil. The phosphorus content of the phosphite-treated soil was lower than that of the phosphate-treated one. TP, inorganic phosphate (Pi), and AP negatively regulated ALP activity, which decreased with increasing phosphate and phosphite fertilizers concentrations. Furthermore, high-throughput sequencing analysis identified 6 phyla and 29 families, which were classified from the altered operational taxonomic units (OTUs) of the soil samples. The redundancy analysis (RDA) revealed that pH, TP, AP and Pi were significantly related to the phoD-harboring bacterial community constructure. The different fertilizer treatments altered the key families, contributing to soil ALP activities. Frankiaceae, Sphingomonadaceae, and Rhizobiaceae positively correlated with ALP activity in phosphite-treated soil. Moreover, the structural equation model (SEM) revealed that ALP activity was affected by the phoD-harboring bacterial community through altered organic phosphorus (Po), AP, total nitrogen (TN), soil organic carbon (SOC), and pH levels under phosphate fertilizer treatment. However, the effect was achieved through positive regulation of pH and AP under phosphite fertilizer. Thus, the changes in soil properties and phoD-harboring bacteria in response to phosphate and phosphite treatments differed in the alfalfa field. This study is the first to report the effects of phosphite on the soil properties of an alfalfa field and provides a strong basis for phosphite utilization in the future. HIGHLIGHTS: – Phosphite and phosphate increase the total phosphorus and available phosphorus. – The pH was the dominant factor influencing the phoD-harboring bacterial community under phosphite fertilizer. – The response of soil properties and phoD-harboring bacterial community to phosphate and phosphite fertilizers differed in the alfalfa field. Frontiers Media S.A. 2022-11-29 /pmc/articles/PMC9746240/ /pubmed/36523837 http://dx.doi.org/10.3389/fmicb.2022.1013896 Text en Copyright © 2022 Li, Wang, Wu, Hu, Cong, Yang, Fu and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Zhenyi
Wang, Jixiang
Wu, Yao
Hu, Jingyun
Cong, Lili
Yang, Chao
Fu, Jinmin
Sun, Juan
Changes in soil properties and the phoD-harboring bacteria of the alfalfa field in response to phosphite treatment
title Changes in soil properties and the phoD-harboring bacteria of the alfalfa field in response to phosphite treatment
title_full Changes in soil properties and the phoD-harboring bacteria of the alfalfa field in response to phosphite treatment
title_fullStr Changes in soil properties and the phoD-harboring bacteria of the alfalfa field in response to phosphite treatment
title_full_unstemmed Changes in soil properties and the phoD-harboring bacteria of the alfalfa field in response to phosphite treatment
title_short Changes in soil properties and the phoD-harboring bacteria of the alfalfa field in response to phosphite treatment
title_sort changes in soil properties and the phod-harboring bacteria of the alfalfa field in response to phosphite treatment
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746240/
https://www.ncbi.nlm.nih.gov/pubmed/36523837
http://dx.doi.org/10.3389/fmicb.2022.1013896
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