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Changes in dendritic complexity and spine morphology following BCG immunization in APP/PS1 mice

Bacillus Calmette – Guerin (BCG) is an immune regulator that can enhance hippocampal synaptic plasticity in rats; however, it is unclear whether it can improve synaptic function in a mouse model with Alzheimer’s disease (AD). We hypothesized that BCG plays a protective role in AD mice and investigat...

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Autores principales: Li, Qingqing, Wang, Xiao, Wang, Zhao Hua, Lin, Zhenzong, Yang, Jieyi, Chen, Jichun, Wang, Rui, Ye, Wenfeng, Li, Ya, Wu, Yingying, Xuan, Aiguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746542/
https://www.ncbi.nlm.nih.gov/pubmed/36113067
http://dx.doi.org/10.1080/21645515.2022.2121568
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author Li, Qingqing
Wang, Xiao
Wang, Zhao Hua
Lin, Zhenzong
Yang, Jieyi
Chen, Jichun
Wang, Rui
Ye, Wenfeng
Li, Ya
Wu, Yingying
Xuan, Aiguo
author_facet Li, Qingqing
Wang, Xiao
Wang, Zhao Hua
Lin, Zhenzong
Yang, Jieyi
Chen, Jichun
Wang, Rui
Ye, Wenfeng
Li, Ya
Wu, Yingying
Xuan, Aiguo
author_sort Li, Qingqing
collection PubMed
description Bacillus Calmette – Guerin (BCG) is an immune regulator that can enhance hippocampal synaptic plasticity in rats; however, it is unclear whether it can improve synaptic function in a mouse model with Alzheimer’s disease (AD). We hypothesized that BCG plays a protective role in AD mice and investigated its effect on dendritic morphology. The results obtained show that BCG immunization significantly increases dendritic complexity, as indicated by the increased number of dendritic intersections and branch points, as well as the increase in the fractal dimension. Furthermore, the number of primary neurites and dendritic length also increased following BCG immunization, which increased the number of spines and promoted maturation. IFN-γ and IL-4 levels increased, while TNF-α levels decreased following BCG immunization; expression levels of p-JAK2, P-STAT3, SYN, and PSD-95 also increased. Therefore, this study demonstrates that BCG immunization in APP/PS1 mice mitigated hippocampal dendritic spine pathology, especially after the third round of immunization. This effect could possibly be attributed to; changes in dendritic arborization and spine morphology or increases in SYN and PSD-95 expression levels. It could also be related to mechanisms of BCG-induced increases in IFN-γ or IL-4/JAK2/STAT3 levels.
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spelling pubmed-97465422022-12-14 Changes in dendritic complexity and spine morphology following BCG immunization in APP/PS1 mice Li, Qingqing Wang, Xiao Wang, Zhao Hua Lin, Zhenzong Yang, Jieyi Chen, Jichun Wang, Rui Ye, Wenfeng Li, Ya Wu, Yingying Xuan, Aiguo Hum Vaccin Immunother Licensed Vaccines – Research Article Bacillus Calmette – Guerin (BCG) is an immune regulator that can enhance hippocampal synaptic plasticity in rats; however, it is unclear whether it can improve synaptic function in a mouse model with Alzheimer’s disease (AD). We hypothesized that BCG plays a protective role in AD mice and investigated its effect on dendritic morphology. The results obtained show that BCG immunization significantly increases dendritic complexity, as indicated by the increased number of dendritic intersections and branch points, as well as the increase in the fractal dimension. Furthermore, the number of primary neurites and dendritic length also increased following BCG immunization, which increased the number of spines and promoted maturation. IFN-γ and IL-4 levels increased, while TNF-α levels decreased following BCG immunization; expression levels of p-JAK2, P-STAT3, SYN, and PSD-95 also increased. Therefore, this study demonstrates that BCG immunization in APP/PS1 mice mitigated hippocampal dendritic spine pathology, especially after the third round of immunization. This effect could possibly be attributed to; changes in dendritic arborization and spine morphology or increases in SYN and PSD-95 expression levels. It could also be related to mechanisms of BCG-induced increases in IFN-γ or IL-4/JAK2/STAT3 levels. Taylor & Francis 2022-09-16 /pmc/articles/PMC9746542/ /pubmed/36113067 http://dx.doi.org/10.1080/21645515.2022.2121568 Text en © 2022 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Licensed Vaccines – Research Article
Li, Qingqing
Wang, Xiao
Wang, Zhao Hua
Lin, Zhenzong
Yang, Jieyi
Chen, Jichun
Wang, Rui
Ye, Wenfeng
Li, Ya
Wu, Yingying
Xuan, Aiguo
Changes in dendritic complexity and spine morphology following BCG immunization in APP/PS1 mice
title Changes in dendritic complexity and spine morphology following BCG immunization in APP/PS1 mice
title_full Changes in dendritic complexity and spine morphology following BCG immunization in APP/PS1 mice
title_fullStr Changes in dendritic complexity and spine morphology following BCG immunization in APP/PS1 mice
title_full_unstemmed Changes in dendritic complexity and spine morphology following BCG immunization in APP/PS1 mice
title_short Changes in dendritic complexity and spine morphology following BCG immunization in APP/PS1 mice
title_sort changes in dendritic complexity and spine morphology following bcg immunization in app/ps1 mice
topic Licensed Vaccines – Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746542/
https://www.ncbi.nlm.nih.gov/pubmed/36113067
http://dx.doi.org/10.1080/21645515.2022.2121568
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