Cargando…

Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow

Pulsatile arterial blood flow plays an important role in vascular system mechanobiology, especially in the study of mechanisms of pathology. Limitations in cost, time, sample size, and control across current in-vitro and in-vivo methods limit future exploration of novel treatments. Presented is the...

Descripción completa

Detalles Bibliográficos
Autores principales: Menkara, Adam, Faryami, Ahmad, Viar, Daniel, Harris, Carolyn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746965/
https://www.ncbi.nlm.nih.gov/pubmed/36512622
http://dx.doi.org/10.1371/journal.pone.0270780
_version_ 1784849482729390080
author Menkara, Adam
Faryami, Ahmad
Viar, Daniel
Harris, Carolyn
author_facet Menkara, Adam
Faryami, Ahmad
Viar, Daniel
Harris, Carolyn
author_sort Menkara, Adam
collection PubMed
description Pulsatile arterial blood flow plays an important role in vascular system mechanobiology, especially in the study of mechanisms of pathology. Limitations in cost, time, sample size, and control across current in-vitro and in-vivo methods limit future exploration of novel treatments. Presented is the verification of a novel reciprocating positive displacement pump aimed at resolving these issues through the simulation of human ocular, human fingertip and skin surface, human cerebral, and rodent spleen organ systems. A range of pulsatile amplitudes, frequencies, and flow rates were simulated using pumps made of 3D printed parts incorporating a tubing system, check valve and proprietary software. Volumetric analysis of 430 total readings across a flow range of 0.025ml/min to 16ml/min determined that the pump had a mean absolute error and mean relative error of 0.041 ml/min and 1.385%, respectively. Linear regression analysis compared to expected flow rate across the full flow range yielded an R(2) of 0.9996. Waveform analysis indicated that the pump could recreate accurate beat frequency for flow ranges above 0.06ml/min at 70BPM. The verification of accurate pump output opens avenues for the development of novel long-term in-vitro benchtop models capable of looking at fluid flow scenarios previously unfeasible, including low volume-high shear rate pulsatile flow.
format Online
Article
Text
id pubmed-9746965
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-97469652022-12-14 Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow Menkara, Adam Faryami, Ahmad Viar, Daniel Harris, Carolyn PLoS One Research Article Pulsatile arterial blood flow plays an important role in vascular system mechanobiology, especially in the study of mechanisms of pathology. Limitations in cost, time, sample size, and control across current in-vitro and in-vivo methods limit future exploration of novel treatments. Presented is the verification of a novel reciprocating positive displacement pump aimed at resolving these issues through the simulation of human ocular, human fingertip and skin surface, human cerebral, and rodent spleen organ systems. A range of pulsatile amplitudes, frequencies, and flow rates were simulated using pumps made of 3D printed parts incorporating a tubing system, check valve and proprietary software. Volumetric analysis of 430 total readings across a flow range of 0.025ml/min to 16ml/min determined that the pump had a mean absolute error and mean relative error of 0.041 ml/min and 1.385%, respectively. Linear regression analysis compared to expected flow rate across the full flow range yielded an R(2) of 0.9996. Waveform analysis indicated that the pump could recreate accurate beat frequency for flow ranges above 0.06ml/min at 70BPM. The verification of accurate pump output opens avenues for the development of novel long-term in-vitro benchtop models capable of looking at fluid flow scenarios previously unfeasible, including low volume-high shear rate pulsatile flow. Public Library of Science 2022-12-13 /pmc/articles/PMC9746965/ /pubmed/36512622 http://dx.doi.org/10.1371/journal.pone.0270780 Text en © 2022 Menkara et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Menkara, Adam
Faryami, Ahmad
Viar, Daniel
Harris, Carolyn
Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow
title Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow
title_full Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow
title_fullStr Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow
title_full_unstemmed Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow
title_short Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow
title_sort applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746965/
https://www.ncbi.nlm.nih.gov/pubmed/36512622
http://dx.doi.org/10.1371/journal.pone.0270780
work_keys_str_mv AT menkaraadam applicationsofanovelreciprocatingpositivedisplacementpumpinthesimulationofpulsatilearterialbloodflow
AT faryamiahmad applicationsofanovelreciprocatingpositivedisplacementpumpinthesimulationofpulsatilearterialbloodflow
AT viardaniel applicationsofanovelreciprocatingpositivedisplacementpumpinthesimulationofpulsatilearterialbloodflow
AT harriscarolyn applicationsofanovelreciprocatingpositivedisplacementpumpinthesimulationofpulsatilearterialbloodflow