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Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow
Pulsatile arterial blood flow plays an important role in vascular system mechanobiology, especially in the study of mechanisms of pathology. Limitations in cost, time, sample size, and control across current in-vitro and in-vivo methods limit future exploration of novel treatments. Presented is the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746965/ https://www.ncbi.nlm.nih.gov/pubmed/36512622 http://dx.doi.org/10.1371/journal.pone.0270780 |
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author | Menkara, Adam Faryami, Ahmad Viar, Daniel Harris, Carolyn |
author_facet | Menkara, Adam Faryami, Ahmad Viar, Daniel Harris, Carolyn |
author_sort | Menkara, Adam |
collection | PubMed |
description | Pulsatile arterial blood flow plays an important role in vascular system mechanobiology, especially in the study of mechanisms of pathology. Limitations in cost, time, sample size, and control across current in-vitro and in-vivo methods limit future exploration of novel treatments. Presented is the verification of a novel reciprocating positive displacement pump aimed at resolving these issues through the simulation of human ocular, human fingertip and skin surface, human cerebral, and rodent spleen organ systems. A range of pulsatile amplitudes, frequencies, and flow rates were simulated using pumps made of 3D printed parts incorporating a tubing system, check valve and proprietary software. Volumetric analysis of 430 total readings across a flow range of 0.025ml/min to 16ml/min determined that the pump had a mean absolute error and mean relative error of 0.041 ml/min and 1.385%, respectively. Linear regression analysis compared to expected flow rate across the full flow range yielded an R(2) of 0.9996. Waveform analysis indicated that the pump could recreate accurate beat frequency for flow ranges above 0.06ml/min at 70BPM. The verification of accurate pump output opens avenues for the development of novel long-term in-vitro benchtop models capable of looking at fluid flow scenarios previously unfeasible, including low volume-high shear rate pulsatile flow. |
format | Online Article Text |
id | pubmed-9746965 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-97469652022-12-14 Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow Menkara, Adam Faryami, Ahmad Viar, Daniel Harris, Carolyn PLoS One Research Article Pulsatile arterial blood flow plays an important role in vascular system mechanobiology, especially in the study of mechanisms of pathology. Limitations in cost, time, sample size, and control across current in-vitro and in-vivo methods limit future exploration of novel treatments. Presented is the verification of a novel reciprocating positive displacement pump aimed at resolving these issues through the simulation of human ocular, human fingertip and skin surface, human cerebral, and rodent spleen organ systems. A range of pulsatile amplitudes, frequencies, and flow rates were simulated using pumps made of 3D printed parts incorporating a tubing system, check valve and proprietary software. Volumetric analysis of 430 total readings across a flow range of 0.025ml/min to 16ml/min determined that the pump had a mean absolute error and mean relative error of 0.041 ml/min and 1.385%, respectively. Linear regression analysis compared to expected flow rate across the full flow range yielded an R(2) of 0.9996. Waveform analysis indicated that the pump could recreate accurate beat frequency for flow ranges above 0.06ml/min at 70BPM. The verification of accurate pump output opens avenues for the development of novel long-term in-vitro benchtop models capable of looking at fluid flow scenarios previously unfeasible, including low volume-high shear rate pulsatile flow. Public Library of Science 2022-12-13 /pmc/articles/PMC9746965/ /pubmed/36512622 http://dx.doi.org/10.1371/journal.pone.0270780 Text en © 2022 Menkara et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Menkara, Adam Faryami, Ahmad Viar, Daniel Harris, Carolyn Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow |
title | Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow |
title_full | Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow |
title_fullStr | Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow |
title_full_unstemmed | Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow |
title_short | Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow |
title_sort | applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746965/ https://www.ncbi.nlm.nih.gov/pubmed/36512622 http://dx.doi.org/10.1371/journal.pone.0270780 |
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