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The world’s largest potato cryobank at the International Potato Center (CIP) – Status quo, protocol improvement through large-scale experiments and long-term viability monitoring

Long-term conservation of Plant Genetic Resources (PGR) is a key priority for guaranteeing food security and sustainability of agricultural systems for current and future generations. The need for the secure conservation of genetic resources collections ex situ is critical, due to rapid and extreme...

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Autores principales: Vollmer, Rainer, Villagaray, Rosalva, Castro, Mario, Cárdenas, José, Pineda, Sandra, Espirilla, Janeth, Anglin, Noelle, Ellis, Dave, Rennó Azevedo, Vânia Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746984/
https://www.ncbi.nlm.nih.gov/pubmed/36523628
http://dx.doi.org/10.3389/fpls.2022.1059817
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author Vollmer, Rainer
Villagaray, Rosalva
Castro, Mario
Cárdenas, José
Pineda, Sandra
Espirilla, Janeth
Anglin, Noelle
Ellis, Dave
Rennó Azevedo, Vânia Cristina
author_facet Vollmer, Rainer
Villagaray, Rosalva
Castro, Mario
Cárdenas, José
Pineda, Sandra
Espirilla, Janeth
Anglin, Noelle
Ellis, Dave
Rennó Azevedo, Vânia Cristina
author_sort Vollmer, Rainer
collection PubMed
description Long-term conservation of Plant Genetic Resources (PGR) is a key priority for guaranteeing food security and sustainability of agricultural systems for current and future generations. The need for the secure conservation of genetic resources collections ex situ is critical, due to rapid and extreme climatic changes which are threatening and reducing biodiversity in their natural environments. The International Potato Center (CIP) conserves one of the most complete and diverse genetic resources collections of potato, with more than 7500 accessions composed of 4900 cultivated potato and 2600 potato wild relative accessions. The clonal conservation of cultivated potato, principally landraces, through in vitro or field collections is indispensable to maintain fixed allelic states, yet it is costly and labor-intensive. Cryopreservation, the conservation of biological samples in liquid nitrogen (-196°C), is considered the most reliable and cost-efficient long-term ex-situ conservation method for clonal crops. Over the last decade, CIP has built one of the largest potato cryobanks worldwide, cyopreserving more than 4000 cultivated potato accessions which represents 84% of the total cultivated potato collection currently conserved at CIP. In approximately, four years the entire potato collection will be cryopreserved. The development of an applied, robust cryopreservation protocol for potato, serves as a model for other clonally maintained crop collections. The CIP cryobank designs experiments with a high number of genetically diverse genotypes (70-100 accessions, seven cultivated species), to obtain reliable results that can be extrapolated over the collection as genotypes can often respond variably to the same applied conditions. Unlike most published reports on cryopreservation of plants, these large-scale experiments on potato are unique as they examine the acclimatization process of in vitro plants prior to, as well as during cryopreservation on up to ten times the number of genotypes conventionally reported in the published literature. As a result, an operational cryopreservation protocol for potato has advanced that works well across diverse potato accessions, not only with reduced processing time and costs, but also with an increased average full-plant recovery rate from 58% to 73% (+LN) for routine cryopreservation. The present article describes the composition of CIP’s cryobank, the cryopreservation protocol, methodology for the dynamic improvement of the operational protocol, as well as data collected on regeneration from long term cryopreserved potatoes.
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spelling pubmed-97469842022-12-14 The world’s largest potato cryobank at the International Potato Center (CIP) – Status quo, protocol improvement through large-scale experiments and long-term viability monitoring Vollmer, Rainer Villagaray, Rosalva Castro, Mario Cárdenas, José Pineda, Sandra Espirilla, Janeth Anglin, Noelle Ellis, Dave Rennó Azevedo, Vânia Cristina Front Plant Sci Plant Science Long-term conservation of Plant Genetic Resources (PGR) is a key priority for guaranteeing food security and sustainability of agricultural systems for current and future generations. The need for the secure conservation of genetic resources collections ex situ is critical, due to rapid and extreme climatic changes which are threatening and reducing biodiversity in their natural environments. The International Potato Center (CIP) conserves one of the most complete and diverse genetic resources collections of potato, with more than 7500 accessions composed of 4900 cultivated potato and 2600 potato wild relative accessions. The clonal conservation of cultivated potato, principally landraces, through in vitro or field collections is indispensable to maintain fixed allelic states, yet it is costly and labor-intensive. Cryopreservation, the conservation of biological samples in liquid nitrogen (-196°C), is considered the most reliable and cost-efficient long-term ex-situ conservation method for clonal crops. Over the last decade, CIP has built one of the largest potato cryobanks worldwide, cyopreserving more than 4000 cultivated potato accessions which represents 84% of the total cultivated potato collection currently conserved at CIP. In approximately, four years the entire potato collection will be cryopreserved. The development of an applied, robust cryopreservation protocol for potato, serves as a model for other clonally maintained crop collections. The CIP cryobank designs experiments with a high number of genetically diverse genotypes (70-100 accessions, seven cultivated species), to obtain reliable results that can be extrapolated over the collection as genotypes can often respond variably to the same applied conditions. Unlike most published reports on cryopreservation of plants, these large-scale experiments on potato are unique as they examine the acclimatization process of in vitro plants prior to, as well as during cryopreservation on up to ten times the number of genotypes conventionally reported in the published literature. As a result, an operational cryopreservation protocol for potato has advanced that works well across diverse potato accessions, not only with reduced processing time and costs, but also with an increased average full-plant recovery rate from 58% to 73% (+LN) for routine cryopreservation. The present article describes the composition of CIP’s cryobank, the cryopreservation protocol, methodology for the dynamic improvement of the operational protocol, as well as data collected on regeneration from long term cryopreserved potatoes. Frontiers Media S.A. 2022-11-29 /pmc/articles/PMC9746984/ /pubmed/36523628 http://dx.doi.org/10.3389/fpls.2022.1059817 Text en Copyright © 2022 Vollmer, Villagaray, Castro, Cárdenas, Pineda, Espirilla, Anglin, Ellis and Rennó Azevedo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Vollmer, Rainer
Villagaray, Rosalva
Castro, Mario
Cárdenas, José
Pineda, Sandra
Espirilla, Janeth
Anglin, Noelle
Ellis, Dave
Rennó Azevedo, Vânia Cristina
The world’s largest potato cryobank at the International Potato Center (CIP) – Status quo, protocol improvement through large-scale experiments and long-term viability monitoring
title The world’s largest potato cryobank at the International Potato Center (CIP) – Status quo, protocol improvement through large-scale experiments and long-term viability monitoring
title_full The world’s largest potato cryobank at the International Potato Center (CIP) – Status quo, protocol improvement through large-scale experiments and long-term viability monitoring
title_fullStr The world’s largest potato cryobank at the International Potato Center (CIP) – Status quo, protocol improvement through large-scale experiments and long-term viability monitoring
title_full_unstemmed The world’s largest potato cryobank at the International Potato Center (CIP) – Status quo, protocol improvement through large-scale experiments and long-term viability monitoring
title_short The world’s largest potato cryobank at the International Potato Center (CIP) – Status quo, protocol improvement through large-scale experiments and long-term viability monitoring
title_sort world’s largest potato cryobank at the international potato center (cip) – status quo, protocol improvement through large-scale experiments and long-term viability monitoring
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746984/
https://www.ncbi.nlm.nih.gov/pubmed/36523628
http://dx.doi.org/10.3389/fpls.2022.1059817
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