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Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors

Discovery of human microbiota is fundamentally changing our perceptions of certain diseases and their treatments. However little is known about the human blood vessel microbiota, it may have important effects on vascular pathological lesions and vascular homograft failure. In our prospective survey...

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Autores principales: Hidi, László, Kovács, Gergely Imre, Szabó, Dóra, Makra, Nóra, Pénzes, Kinga, Juhász, János, Sótonyi, Péter, Ostorházi, Eszter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9747773/
https://www.ncbi.nlm.nih.gov/pubmed/36530429
http://dx.doi.org/10.3389/fcimb.2022.1056319
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author Hidi, László
Kovács, Gergely Imre
Szabó, Dóra
Makra, Nóra
Pénzes, Kinga
Juhász, János
Sótonyi, Péter
Ostorházi, Eszter
author_facet Hidi, László
Kovács, Gergely Imre
Szabó, Dóra
Makra, Nóra
Pénzes, Kinga
Juhász, János
Sótonyi, Péter
Ostorházi, Eszter
author_sort Hidi, László
collection PubMed
description Discovery of human microbiota is fundamentally changing our perceptions of certain diseases and their treatments. However little is known about the human blood vessel microbiota, it may have important effects on vascular pathological lesions and vascular homograft failure. In our prospective survey study fourteen femoral arteries, harvested from donors in multi-organ donations, were examined using the V3-V4 region 16S rRNA sequencing method. The most abundant phyla in the human vascular microbiota were Proteobacteria, Firmicutes and Actinobacteria. At the genus level, the most abundant taxa were Staphylococcus, Corynebacterium, Pseudomonas, Bacillus, Acinetobacter and Propionibacterium. Of the bacterial taxa that have an indirect effect on the development of atherosclerosis, we found Porphyromonas gingivalis, Prevotella nigrescens and Enterobacteriaceae spp. with different abundances in our samples. Of the bacteria that are more common in the intestinal flora of healthy than of atherosclerosis patients, Roseburia and Ruminococcus occurred in the majority of samples. The human arterial wall has a unique microbiota that is significantly different in composition from that of other areas of the body. Our present study provides a basis for ensuing research that investigates the direct role of the microbiota in vascular wall abnormalities and the success of vascular allograft transplantations.
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spelling pubmed-97477732022-12-15 Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors Hidi, László Kovács, Gergely Imre Szabó, Dóra Makra, Nóra Pénzes, Kinga Juhász, János Sótonyi, Péter Ostorházi, Eszter Front Cell Infect Microbiol Cellular and Infection Microbiology Discovery of human microbiota is fundamentally changing our perceptions of certain diseases and their treatments. However little is known about the human blood vessel microbiota, it may have important effects on vascular pathological lesions and vascular homograft failure. In our prospective survey study fourteen femoral arteries, harvested from donors in multi-organ donations, were examined using the V3-V4 region 16S rRNA sequencing method. The most abundant phyla in the human vascular microbiota were Proteobacteria, Firmicutes and Actinobacteria. At the genus level, the most abundant taxa were Staphylococcus, Corynebacterium, Pseudomonas, Bacillus, Acinetobacter and Propionibacterium. Of the bacterial taxa that have an indirect effect on the development of atherosclerosis, we found Porphyromonas gingivalis, Prevotella nigrescens and Enterobacteriaceae spp. with different abundances in our samples. Of the bacteria that are more common in the intestinal flora of healthy than of atherosclerosis patients, Roseburia and Ruminococcus occurred in the majority of samples. The human arterial wall has a unique microbiota that is significantly different in composition from that of other areas of the body. Our present study provides a basis for ensuing research that investigates the direct role of the microbiota in vascular wall abnormalities and the success of vascular allograft transplantations. Frontiers Media S.A. 2022-11-30 /pmc/articles/PMC9747773/ /pubmed/36530429 http://dx.doi.org/10.3389/fcimb.2022.1056319 Text en Copyright © 2022 Hidi, Kovács, Szabó, Makra, Pénzes, Juhász, Sótonyi and Ostorházi https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Hidi, László
Kovács, Gergely Imre
Szabó, Dóra
Makra, Nóra
Pénzes, Kinga
Juhász, János
Sótonyi, Péter
Ostorházi, Eszter
Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors
title Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors
title_full Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors
title_fullStr Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors
title_full_unstemmed Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors
title_short Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors
title_sort human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9747773/
https://www.ncbi.nlm.nih.gov/pubmed/36530429
http://dx.doi.org/10.3389/fcimb.2022.1056319
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